12 research outputs found
Microendoscopic laminotomy versus conventional laminoplasty for cervical spondylotic myelopathy: 5-year follow-up study
The combination of strong immunohistochemical mtTFA expression and a high survivin index predicts a shorter diseasespecific survival in pancreatic ductal adenocarcinoma
Mitochondrial transcription factor A
(mtTFA) plays a crucial role in both the transcription and
maintenance of mitochondrial DNA. A high expression
of mtTFA has been demonstrated in several solid tumors,
and is closely associated with cancer cell
survival/apoptosis and growth. However, its expression
pattern in pancreatic ductal adenocarcinoma (PAC)
remains to be elucidated. Additionally, our groups have
recently revealed that a subset of apoptosis-related genes
is strongly regulated by mtTFA, and that two putative
mtTFA binding sites are present in the promoter region
of the survivin gene, which is a member of the inhibitorof-apoptosis protein family. We therefore investigated
the correlation of the immunohistochemical mtTFA
expression and the survivin index with various
clinicopathological variables and the prognosis, using 70
paraffin-embedded tumor samples from patients with
surgically-resected PAC. The mtTFA expression or
survivin index was considered to be strong or high when
≥30% or 10% of the PAC cells showed positive staining,
respectively. Strong mtTFA expression and/or a high
survivin index was revealed to have a significant
relationship to a pathologically high tumor grading and
advanced tumor stage. Moreover, mtTFA showed
significantly high co-expression with survivin.
Univariate and multivariate analyses demonstrated that
both the strong mtTFA expression and high survivin
index groups had significantly shorter survival rates,
especially within the first two years postoperatively. The
combination of strong mtTFA expression and a high
survivin index may predict a poor prognosis in patients
with PAC, and these new biomarkers might offer useful
information for the early clinical management
Hidden coexisting pathology diagnosed after cervical surgery in patients with degenerative cervical myelopathy or myeloradiculopathy: A case series report
Sarcopenia is a risk factor for falling in independently living Japanese older adults: A 2-year prospective cohort study of the GAINA study
Perioperative complications of anterior cervical decompression with fusion in patients with ossification of the posterior longitudinal ligament: a retrospective, multi-institutional study
Age independency of mobility decrease assessed using the Locomotive Syndrome Risk Test in elderly with disability: a cross-sectional study
Perioperative complications of anterior cervical decompression with fusion in patients with ossification of the posterior longitudinal ligament: a retrospective, multi-institutional study
Development and evaluation of a video exercise program for locomotive syndrome in the elderly
Genetic insights into ossification of the posterior longitudinal ligament of the spine
Ossification of the posterior longitudinal ligament of the spine (OPLL) is an intractable disease leading to severe neurological deficits. Its etiology and pathogenesis are primarily unknown. The relationship between OPLL and comorbidities, especially type 2 diabetes (T2D) and high body mass index (BMI), has been the focus of attention; however, no trait has been proven to have a causal relationship. We conducted a meta-analysis of genome-wide association studies (GWASs) using 22,016 Japanese individuals and identified 14 significant loci, 8 of which were previously unreported. We then conducted a gene-based association analysis and a transcriptome-wide Mendelian randomization approach and identified three candidate genes for each. Partitioning heritability enrichment analyses observed significant enrichment of the polygenic signals in the active enhancers of the connective/bone cell group, especially H3K27ac in chondrogenic differentiation cells, as well as the immune/hematopoietic cell group. Single-cell RNA sequencing of Achilles tendon cells from a mouse Achilles tendon ossification model confirmed the expression of genes in GWAS and post-GWAS analyses in mesenchymal and immune cells. Genetic correlations with 96 complex traits showed positive correlations with T2D and BMI and a negative correlation with cerebral aneurysm. Mendelian randomization analysis demonstrated a significant causal effect of increased BMI and high bone mineral density on OPLL. We evaluated the clinical images in detail and classified OPLL into cervical, thoracic, and the other types. GWAS subanalyses identified subtype-specific signals. A polygenic risk score for BMI demonstrated that the effect of BMI was particularly strong in thoracic OPLL. Our study provides genetic insight into the etiology and pathogenesis of OPLL and is expected to serve as a basis for future treatment development