Large Eddy Simulations (LES) towards a comprehensive understanding of Ducted Fuel Injection concept in non-reacting conditions

The diesel combustion research is increasingly focused on Ducted Fuel Injection (DFI), a promising concept to abate engine-out soot emissions in Compression-Ignition engines. A large set of experiments and numerical simulations, at medium-low computational cost, showed that the duct adop- tion in front of the injector nozzle activates several soot mitigation mechanisms, leading to quasi-zero soot formation in several engine-like operating conditions. However, although the simplified CFD mod- elling so far played a crucial role for the preliminary understanding of DFI technology, a more accurate turbulence description approach, combined with a large set of numerical experiments for statistical pur- poses, is of paramount importance for a robust knowledge on the DFI physical behavior. In this context, the present work exploits the potential of Large Eddy Simulations (LES) to analyze the non-reacting spray of DFI configuration compared with the unconstrained spray. For this purpose, a previously developed spray model, calibrated and validated in the RANS framework against an exten- sive amount of experimental data related to both free spray and DFI, has been employed. This high- fidelity simulation model has been adapted for LES, firstly selecting the best grid settings, and then carrying out several numerical experiments for both spray configurations until achieving a satisfying statistical convergence. With this aim, the number of independent samples for the averaging procedure has been increased exploiting the axial symmetry characteristics of the present case study. The relia- bility of this methodology has been herein proven, highlighting an impressive runtime saving without any remarkable worsening of the accuracy level. Thanks to this approach, a detailed description of the main DFI-enabled soot mitigation mechanisms has been achieved, bridging the still open knowledge gap in the physical understanding of the impact of spray-duct interaction

Investigation of Ducted Fuel Injection Implementation in a Retrofitted Light-Duty Diesel Engine through Numerical Simulation

Ducted Fuel Injection (DFI) is a concept of growing interest to abate soot emissions in diesel combustion, based on a small duct within the combustion chamber in front of the injector nozzle. Despite the impressive potential of the DFI has been proven in literature, its application for series production and the complexity for the adaptation of existing compression-ignition (CI) engines need to be extensively investigated. In this context, the aim of this study is to numerically assess the potential of DFI implementation in a CI engine for light-duty applications, highlighting the factors which can limit or facilitate its integration in existing combustion chambers. The numerical model for combustion simulation was based on a 1D/3D-CFD coupled approach relying on a calibrated spray model, extensively validated against experimental data. Once assessed the coupling procedure by comparing the numerical results with experimental in-cylinder pressure and heat release rate data for both low and high load operating conditions, the duct impact was investigated introducing it in the computational domain. It was observed that DFI did not yield any significant advantage to engine-out soot emissions and fuel consumption with the existing combustion system. Although the soot formation was generally reduced, the soot oxidation process was partially inhibited by the duct adoption maintaining fixed the engine calibration, suggesting the need for complete optimization of the combustion system design. On the other hand, a preliminary variation of engine calibration highlighted several beneficial trends for DFI, whose operation improved with a simplified injection strategy. Present numerical results indicate that DFI retrofit solutions without specific optimization of the combustion system design do not guarantee soot reduction. Nevertheless, wide room for improvement remains in terms of DFI-targeted combustion chamber design and engine calibration towards the complete success of this technology for soot-free CI engines

Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

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Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field