149 research outputs found
PDZ Domains and the Politics of Polarity in Lymphocytes
In this issue of Immunity, Ludford-Menting et al. (2005) show that scaffold proteins previously implicated in the control of epithelial cell polarity also play an important role in determining the macroscopic organization of the engaged T cell
Expression of the CD6 T lymphocyte differentiation antigen in normal human brain
Antigens shared by the immune and central nervous systems (CNS) have been described repeatedly.
The present study reports the expression of the CD6 lymphocyte differentiation antigen in normal human
brain evidenced by immunohistochemistry and Northern blot analysis. A panel of various anti-CD6
monoclonal antibodies (mabs) tested on serial cryostat sections identified CD6-positive cells randomly
scattered in parenchyma of all examined brain areas. Northern blot analysis with a highly sensitive cRNA
probe revealed a 3.1 kb CD6-specific mRNA in various brain regions, especially in basalganglia and cortex
cerebellum. Staining with mabs raised against different hematopoietic cell types, as well as hybridization
with probes specific for the ß- and y-T cell receptor (TCR) chains support the notion that CD6 is
expressed by original brain cells. The nature of the CD6-positive cell type and possible functions of shared
antigens in immune and nervous systems are discusse
Spinophilin and the immune synapse
Extensive alterations in cellular organization are known to accompany the responses of sensitized T cells to target cells presenting an antigen of interest. Now, equally if not more dramatic changes are found to take place in cells presenting an antigen. With the help of a spinophilin-GFP fusion protein, Bloom et al. (Bloom, O., J.J. Unternaehrer, A. Jiang, J.-S. Shin, L. Delamarre, P. Allen, and I. Mellman. 2008. J. Cell Biol. 181:203–211) have captured a remarkable polarization of the cellular architecture of dendritic cells presenting an antigen to T cells
A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance
<p>Abstract</p> <p>Background</p> <p>Quantitative polymerase chain reaction (QPCR) is a widely applied analytical method for the accurate determination of transcript abundance. Primers for QPCR have been designed on a genomic scale but non-specific amplification of non-target genes has frequently been a problem. Although several online databases have been created for the storage and retrieval of experimentally validated primers, only a few thousand primer pairs are currently present in existing databases and the primers are not designed for use under a common PCR thermal profile.</p> <p>Results</p> <p>We previously reported the implementation of an algorithm to predict PCR primers for most known human and mouse genes. We now report the use of that resource to identify 17483 pairs of primers that have been experimentally verified to amplify unique sequences corresponding to distinct murine transcripts. The primer pairs have been validated by gel electrophoresis, DNA sequence analysis and thermal denaturation profile. In addition to the validation studies, we have determined the uniformity of amplification using the primers and the technical reproducibility of the QPCR reaction using the popular and inexpensive SYBR Green I detection method.</p> <p>Conclusion</p> <p>We have identified an experimentally validated collection of murine primer pairs for PCR and QPCR which can be used under a common PCR thermal profile, allowing the evaluation of transcript abundance of a large number of genes in parallel. This feature is increasingly attractive for confirming and/or making more precise data trends observed from experiments performed with DNA microarrays.</p
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A pathway sensor for genome-wide screens of intracellular proteolytic cleavage
A new system based on non-conventional secretion of the luciferase from Gaussia princeps (GLUC) can be used to detect intracellular proteolysis in vivo
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