A new system based on non-conventional secretion of the luciferase from Gaussia princeps (GLUC) can be used to detect intracellular proteolysis in vivo

He, Wei-Wu

Ketteler, Robin

Kovacs, Karl F

Seed, Brian

Sun, Zairen

Genome Biology

English

PubMed

Robin Ketteler

Zairen Sun

Karl F Kovacs

Wei-Wu He

Brian Seed

Springer - Publisher Connector

A pathway sensor for genome-wide screens of intracellular proteolytic cleavage

Protein cleavage is a central event in many regulated biological processes. We describe a system for detecting intracellular proteolysis based on non-conventional secretion of Gaussia luciferase (GLUC). GLUC exits the cell without benefit of a secretory leader peptide, but can be anchored in the cell by fusion to \(\beta\)-actin. By including protease cleavage sites between GLUC and \(\beta\)-actin, proteolytic cleavage can be detected. Using this assay, we have identified regulators of autophagy, apoptosis and \(\beta\)-actin cleavage

Harvard University - DASH 

A Pathway Sensor for Genome-Wide Screens of Intracellular Proteolytic Cleavage

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A pathway sensor for genome-wide screens of intracellular proteolytic cleavage

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