Hygiene, Sanitation, and Water: Forgotten Foundations of Health

As the first article in a four-part PLoS Medicine series on water and sanitation, Jamie Bartram and Sandy Cairncross argue that the massive burden of ill health associated with poor hygiene, sanitation, and water supply demands more attention from health professionals and policymakers

The influence of cultivation methods on Shewanella oneidensis physiology and proteome expression

High-throughput analyses that are central to microbial systems biology and ecophysiology research benefit from highly homogeneous and physiologically well-defined cell cultures. While attention has focused on the technical variation associated with high-throughput technologies, biological variation introduced as a function of cell cultivation methods has been largely overlooked. This study evaluated the impact of cultivation methods, controlled batch or continuous culture in bioreactors versus shake flasks, on the reproducibility of global proteome measurements in Shewanellaoneidensis MR-1. Variability in dissolved oxygen concentration and consumption rate, metabolite profiles, and proteome was greater in shake flask than controlled batch or chemostat cultures. Proteins indicative of suboxic and anaerobic growth (e.g., fumarate reductase and decaheme c-type cytochromes) were more abundant in cells from shake flasks compared to bioreactor cultures, a finding consistent with data demonstrating that “aerobic” flask cultures were O2 deficient due to poor mass transfer kinetics. The work described herein establishes the necessity of controlled cultivation for ensuring highly reproducible and homogenous microbial cultures. By decreasing cell to cell variability, higher quality samples will allow for the interpretive accuracy necessary for drawing conclusions relevant to microbial systems biology research

Collective evil and individual pathology: The depoliticization of violence against Afghan civilians

This article explores how the violence against Afghan civilians carried out by the Taliban and US ‘rogue’ soldiers has been accounted for as the product of, respectively, collective evil and individual pathology. These two seemingly contending explanations, it is argued, are part of the same strategy of depoliticization, which aims to provide support and legitimacy for the US-led war in Afghanistan. The article discusses how the genealogy of the discourse of collective evil surrounding the Taliban can be traced to an Orientalist political theodicy, which frames the Taliban as ‘children of a lesser God’ – that is, as fanatical puppets at the mercy of a violent God – and how the discourse of individual pathology surrounding the unsanctioned violence of US soldiers is instrumental to exempt military and civilian leadership from collusion and responsibility. The article challenges this latter narrative of individual blame by discussing how killing, torture and desecration of bodies are at the heart of warfare. Hence, it is concluded, the language of collective evil and individual pathology are part of the same strategy of depoliticization, which aims to silence political contestation and conceal the dehumanizing aspect of war, its structural production of violence, and the complex and dispersed nature of responsibility

Screen wars, star wars, and sequels

Motion-picture profit, Nonparametric regression, Hollywood economics, L82, C14,