Cellular composition of the spleen from naive and <i>T. cruzi</i>- infected mice.

<div><p>Single spleen cell suspensions from naive mice (A) and from mice infected on day 24 with <i>T. cruzi</i> (B) were analysed by flow cytometry.</p> <p>Cells were stained for both CD4 and CD8, for CD19, for CD11b, or with propidium iodide (PI) and Annexin V (AxV).</p> <p>Numbers indicate percentage of cells expressing the respective marker.</p> <p>B6, C57BL/6 mice; F1, B6D2F1 mice.</p></div

Murine <i>T. cruzi</i> susceptibility loci map to Chromosomes 5, 13, and 17.

<div><p>Nine microsatellite markers from the three candidate genomic regions previously identified as suceptibility loci <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000057#pone.0000057-Graefe2" target="_blank">[30]</a> were typed in an additional 22 <i>T. cruzi</i>- susceptible B6xF1 backcross mice (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000057#pone-0000057-t003" target="_blank">table 3</a> for respective markers).</p> <p>Single-point (dashed line) and multi-point (solid line) calculations of χ² are shown for all 68 susceptible backcross mice including the 46 from the original study.</p> <p>Dotted lines at χ² = 16.56 and χ² = 8.74 indicate thresholds for suggestive and significant linkage, respectively.</p></div

Splenic architecture and cellular composition in susceptible B6 and in resistant F1 mice.

<div><p>Spleens from B6 mice (left panel) and from F1 mice (right panel) were either fixed in paraformaldehyde, embedded in paraffin, and subsequently stained with hematoxylin and eosin (A–D), or they were shock frozen in liquid nitrogen and analysed by immunohistochemistry (C–M).</p> <p>Sections are from naive mice (A, B) or from <i>T. cruzi</i>- infected mice (C–M) at the indicated day of infection.</p> <p>Cryosections were stained for CD4 (E, F), CD19 (G, H), CD8 (I, K), or by the TUNEL reaction (L, M).</p> <p>Micrographs A–K at original magnification 100×, micrographs L, M and insert in D at 400×; inset in D shows centre of a secondary follice.</p></div

Experimental <b><i>T. cruzi</i></b><b> infection in susceptible B6 and in resistant F1 mice.</b>

<div><p>Mice were infected with 10⁴<i>T. cruzi</i> trypomastigotes into the peritoneum.</p> <p>Survival was monitored up to day 60.</p> <p>Parasitaemia was determined in a Neubauer chamber.</p> <p>Tissue parasite loads in spleen, liver, heart and skeletal muscle were determined by real time PCR and are expressed as arbitrary log units of <i>T. cruzi</i> DNA relative to quantity of murine β-actin DNA.</p></div