Perinatal outcomes of females according to trimester of exposure to earthquake.

<p>Perinatal outcomes of females according to trimester of exposure to earthquake.</p

Flow diagram of selection of data.

<p>Flow diagram of selection of data.</p

Perinatal outcomes of males according to trimester of exposure to earthquake.

<p>Perinatal outcomes of males according to trimester of exposure to earthquake.</p

Maternal and newborn characteristics and perinatal outcomes by year and gender.

<p>Maternal and newborn characteristics and perinatal outcomes by year and gender.</p

Supplementary_Figure_1 - Reduced FOXM1 Expression Limits Trophoblast Migration and Angiogenesis and Is Associated With Preeclampsia

<p>Supplementary_Figure_1 for Reduced FOXM1 Expression Limits Trophoblast Migration and Angiogenesis and Is Associated With Preeclampsia by Lara J. Monteiro, Sofia Cubillos, Marianela Sanchez, Stephanie Acuña-Gallardo, Pía Venegas, Valentina Herrera, Eric W.-F. Lam, Manuel Varas-Godoy, and Sebastián E. Illanes in Reproductive Sciences</p

Aromatase is downregulated in JEG–3 cell line in response to hypoxia.

<p>JEG–3 cell line was exposed either to 8% or to 1% O₂ in an hypoxic chamber for 0, 4, 8, 16 and 24 h. <b>A</b>. Cells were collected at indicated times and protein lysates analyzed by western blot to determine the protein expression levels of aromatase, HIF–1α and β-Tubulin. Upper panel shows representative western blots and lower panels show aromatase and HIF–1α proteins densitometry data normalized to β-Tubulin loading control from n = 3 experiments. Data are shown in arbitrary units (A.U.) ±SEM. <b>B</b>. Cells were collected at indicated times and analyzed by qRT–PCR to determine aromatase mRNA transcript levels. Statistical analyses were performed using Student’s <i>t</i>-test and compared with the correspondent time point in the control, 8% O₂, cells. Columns are the mean of four independent experiments in duplicate; Data are reported as mean±SEM. *<i>P</i>≤0.05; **<i>P</i>≤0.01; *** <i>P</i>≤0.001, significant; n.s., non-significant.</p

Clinical characteristics of controls and preeclampsia patients at different times of gestation.

<p>Values are given as Mean±SEM. Statistical significance was assessed using Mann Whitney test and Fisher's exact test.</p><p>*p≤0.05</p><p>**p≤0.01</p><p>***p≤0.001</p><p>Clinical characteristics of controls and preeclampsia patients at different times of gestation.</p

Effect of hypoxia on placental aromatase expression <i>in vivo</i>.

<p><b>A</b>. Pups of timed-pregnant rabbits derived either from one uterine horn which placental spiral arteries had been ligated or from the non-ligated placentas on the contralateral horn (control horn, n = 17) were weighted <b>B</b>. and RNA was extracted from the correspondent placentas. Aromatase mRNA levels were analyzed by qRT-PCR in the control (n = 17) and hypoxic horn (n = 10). At least one hypoxic and one no-hypoxic placenta were collected from each of the 6 pregnant rabbits. Data are reported as mean±SEM. Statistical analysis was conducted using Mann-Whitney test. *<i>P</i>≤0.05; **<i>P</i>≤0.01, significant.</p

Aromatase metabolite levels are dysregulated in PE patients.

<p>Aromatase functionality was measured in PE and normotensive pregnancies. The levels of aromatase precursors and metabolites including, <b>A</b>. testosterone, <b>B</b>. androstenedione, <b>C</b>. 17-β-estradiol, and <b>D</b>. estrone were measured by RIA in maternal serum samples collected at 32–36 weeks of gestation. Also shown are <b>E</b>. 17-β-estradiol/testosterone and <b>F</b>. estrone/androstenedione ratios. Data are reported as mean±SEM from 32 controls and 16 PE patients. Statistical analysis was performed using Mann-Whitney test. *<i>P</i>≤0.05; **<i>P</i>≤0.01, significant; n.s., non-significant.</p