Immunohistochemical characterization of feline lymphoplasmacytic anterior uveitis

OBJECTIVE: To characterize the immune cells present in different forms of feline anterior uveitis. SAMPLES: Eyes were obtained from 49 cats diagnosed with chronic idiopathic lymphoplasmacytic anterior uveitis, 7 cats with feline infectious peritonitis (FIP), and 9 cats euthanized for nonocular disease. METHODS: H&E sections were scored on the level of infiltrate in the anterior uvea. Immunohistochemistry was performed for FoxP3, CD3, and IL-17A, and positive cells were quantified in multiple images of each sample. A generalized estimating equation tested for an association between the level of inflammation and the prevalence of these cell types. RESULTS: Cells stained positive for IL-17A in idiopathic uveitis but not in FIP samples. We found significantly fewer FoxP3+and CD3+cells in low-grade compared with high-grade inflammation in idiopathic uveitis or FIP samples (P values all <.005), but no difference between FIP and high-grade samples. CONCLUSIONS: Idiopathic, but not FIP-associated, uveitis appears to have Th17 cell involvement. The numbers of FoxP3+and CD3+T-cells present appear directly correlated; thus, the severity of disease does not appear directly determined by the numbers of regulatory cells

Synthesis of highly oriented gold thin films by a UV-irradiation route

A synthetic route is presented for the preparation of a gold film in the presence of UV-radiation. Methoxypolyethylene glycol, a water-soluble polymer, was used as the reducing agent of gold ions in the presence of an ultra-violet source, and gold nanoparticles resulted. During stirring, a centrifugal force is generated at the center of the solution (at the point where the meniscus is at its lowest level with respect to the vertical orthogonal). At this point, the nanoparticles coalesce and form a self-assembly of smaller subunits that ultimately forms a film like network

Controlled Synthesis of CeO₂NS-Au-CdSQDs Ternary Nanoheterostructure: A Promising Visible Light Responsive Photocatalyst for H₂ Evolution

With the advancement of promising multifaceted powdered photocatalytic systems, problems related to environmental pollution and energy requirements have been addressed to a significant extent. The major reason for this great achievement lies in the combined effect of both structure modification and integration of different functional materials. Here, we report a ternary hybrid containing wide band gap CeO₂ nanosheets with CdSQDs and Au nanoparticles, incorporated between this type II heterostructure through simple chemical reduction methods. Structural and morphological characterization of the fabricated samples was carried out by XRD, XPS, and TEM analysis. From a series of optical and photoelectrochemical measurements, it was found that the incorporation of Au nanoparticles into the interfaces of CeO₂ and CdSQDs was the major cause of the enhancement in the photocatalytic activity. Au nanoparticles play a dual character by acting as a mediator and also inject hot electrons through LSPR (light-induced surface plasmon resonance) effects in the ternary hybrid. The photocatalytic activity of the fabricated samples was tested toward H₂ evolution, where the ternary hybrid CeO₂NS-Au-CdSQDs lead the activity sequence with 499 μmol/2 h followed by the binary and neat counterparts. From the Mott–Shottky and linear sweep voltammetry measurements, a heterostructure relay mechanism was predicted where electrons from CdSQDs flow to the surface of CeO₂ via Au. The novelty of this work is that it provides useful information about the synergistic effect among three functional components, integrated in a nanosheet structured system, as the basic requirement for constructing good heterostructures in powdered photocatalytic systems

Prospects for Lower Carbon Routes for Conversion of Natural Gas to Energy

Nickel-copper catalysts based on supports comprising zeolite (ZSM-5) show enhanced capacity for carbon deposition from the high-temperature decomposition of methane, with very pure hydrogen being a co-product. It has been found that the zeolite-based catalysts are superior to those using alumina or silica as supports with considerably enhanced carbon deposition capacity. The zeolite morphology appears to play a key role in determining the carbon deposition capacity, with flat micrometer-sized crystallites showing the best performance compared to spherical or nanocubic morphologies. It also seems clear that there are also differences in the detailed interaction between the metal centers and the support, as evidenced by changes in the temperature-programmed reduction behavior of the various systems, and this may be related to the enhanced carbon capacity shown

Haemangiosarcoma in the uterine remnant of a spayed female dog

A 11-year-old, female, spayed greyhound was presented with a haemorrhagic discharge from the vulva. Clinical examination, vaginoscopy and a computed tomography scan showed an irregular egg-sized mass in the region of the cervix and uterine stump. An endoscopic grab biopsy (incisional) suggested a malignant mesenchymal tumour. Following this, surgical excision of the cranial vagina, cervix and the uterine remnant was performed. The final diagnosis of haemangiosarcoma was based on histological examination of the larger excisional biopsy specimen and was confirmed by positive immunolabelling of the neoplastic endothelial cells for the von Willebrand factor.</p

Study of carbon monoxide hydrogenation over Au supported on zinc oxide catalysts

Recently, there has been a marked increase in the interest shown in catalysis by gold. It is now recognised that gold has unique properties as a catalyst for many reactions with pre-eminence in the oxidation of carbon monoxide. However, it is also possible that supported gold catalysts can be used for other reactions involving carbon monoxide, for example the water gas shift reaction. Supported gold catalysts have also been shown to be effective for hydrogenation reactions. This paper sets out the possible use of gold as a catalyst for the hydrogenation of carbon monoxide. In particular, we describe the preparation and characterisation of Au/ZnO and Au/ZnO/Al2O 3 as catalysts for the hydrogenation of carbon monoxide to methanol