2 research outputs found
Investigating the role of RNAi and epigenetic modifications in the basidiomycetes yeast Cryptococcus deneoformans
The basidiomycete yeasts from the Cryptococcus neoformans/gattii species complex are
major fungal pathogens and are particularly prevalent in the developing world. The
species within the complex are rapidly evolving, with several species loosing genes
encoding proteins required for silencing by the RNA interference (RNAi) pathway which
correlate with an increase virulence. The species C. deneoformans, however, has
retained all five of the core RNAi components (Rdp1, Ago1, Ago2, Dcr1, Dcr2), and
has been shown to have a functional RNAi pathway involved in the silencing of
transposable elements (TEs). Centromeric TEs have also been shown to coincide with
DNA methylation in C. deneoformans, and also with H3K9 methylation in neighbouring
species C. neoformans. Here I look at the relationship between these three potential
mechanisms of silencing in C. deneoformans, RNAi, DNA methylation and H3K9
methylation, focussing on how RNAi interacts with both methylation marks in TE
regulation.
Identification of the H3K9 methyltransferase Clr4 and H3K9me2-ChIP confirmed the
presence of H3K9 methylation at the centromeres in C. deneoformans. Analysis of strains
with deletions of core RNAi components revealed wild-type levels of centromeric H3K9
methylation, confirming that RNAi is not required for maintenance of this
heterochromatin mark. Analysis of transcript levels at RNAi target sites showed no
difference between wild-type and RNAi deficient strains. This suggests that RNAi
silences targets through a post-transcriptional gene silencing (PTGS) method out with
RNA degradation.
To investigate the role of RNAi in suppressing transposon activity, mutation rate assays
were carried out by screening for spontaneous 5-FOA resistance that results from
disruption of the URA3 or URA5 genes. Strains lacking both H3K9 methylation and DNA
methylation (clr4Δdnmt5Δ) had the highest drug resistance rates. PCR screening
determined if 5-FOA resistance was due to transposon insertion into URA3 or URA5,
and both T1 and T2 DNA transposon insertions were identified. The rate of inserts
identified within rdp1Δ and clr4Δdnmt5Δ strains was significantly higher than in WT,
showing increased transposon mobility in both strains. Analysis of DNA transposable
element expression showed large variance between replicate cultures but suggested
that T3 may be regulated by an RNAi-independent mechanism, unlike T1 and T2 where
suppression appears dependent on Rdp1. Analysis of retrotransposon copy numbers
showed no significant increase in any strains tested when compared to WT. Overall this
shows a potential role for H3K9 and/or DNA methylation in controlling transposon
mobility alongside RNAi.
Finally, analysis was carried out into the roles of both Argonaute proteins within C.
deneoformans, as Ago2 is frequently lost within the species complex, and is not present
within neighbouring species C. neoformans. Mass spectrometry of tagged proteins
showed that each Ago binds to a different subset of proteins, suggesting a different
role for each protein within the RNAi pathway. Deletion of Gwo1, the main Ago1
interactor, increases the interaction of Ago1 with Ago2.
The work undertaken here contributes to the further understanding of the interaction
between RNAi and the DNA and H3K9 methylation silencing pathway in C.
deneoformans and shed lights on the different roles of the two Argonaute proteins in
this species
Prion-like domains drive CIZ1 assembly formation at the inactive X chromosome
CIZ1 forms large assemblies at the inactive X chromosome (Xi) in female fibroblasts in an Xist lncRNA-dependent manner and is required for accurate maintenance of polycomb targets genome-wide. Here we address requirements for assembly formation and show that CIZ1 undergoes two direct interactions with Xist, via independent N- and C-terminal domains. Interaction with Xist, assembly at Xi, and complexity of self-assemblies formed in vitro are modulated by two alternatively spliced glutamine-rich prion-like domains (PLD1 and 2). PLD2 is dispensable for accumulation at existing CIZ1–Xi assemblies in wild-type cells but is required in CIZ1-null cells where targeting, assembly, and enrichment for H3K27me3 and H2AK119ub occur de novo. In contrast, PLD1 is required for both de novo assembly and accumulation at preexisting assemblies and, in vitro, drives formation of a stable fibrillar network. Together they impart affinity for RNA and a complex relationship with repeat E of Xist. These data show that alternative splicing of two PLDs modulates CIZ1’s ability to build large RNA–protein assemblies