Grx1 expression in lung tissue and primary macrophages of mice exposed to cigarette smoke.

<p>(A) Grx1 mRNA expression corrected for HPRT mRNA expression in lung tissue of mice exposed to air and cigarette smoke, represented as mean ± SD. (B) Fluorescent Grx1 staining in primary macrophages after 24 hours of control or 0.5% cigarette smoke extract exposure. The green staining represents Grx1 protein expression, whereas blue represents the nuclear DAPI staining. Quantification of fluorescent Grx1 staining is expressed as RFI in (C).</p

BAL fluid cell counts and differentials in wild type versus Grx1 KO mice exposed to air and smoke.

<p>Total BAL fluid cells (A), total numbers of macrophages (B), neutrophils (C), dendritic cells (D), CD3+ cells (F), CD4+ cells (G) and CD8+ cells (H) in BAL fluid represented as mean ± SD. * represents p<0.05 between air and smoke exposed mice, # represents p<0.05 between WT smoke and KO smoke.</p

BAL fluid cytokines in wild type versus Grx1 KO mice exposed to air and cigarette smoke.

<p>Measurement of cytokines in the BAL fluid by multiplex: IL 12 (A), GCSF (B), MCP1 (C), KC (D), RANTES (E) and MIP1α (F) expressed in pg/ml and represented as mean ± SD. * represents p<0.05 between air and smoke exposed mice, # represents p<0.05 between WT smoke and KO smoke and $ represents p<0.05 between WT air and Grx1 KO air.</p

S-glutathionylation in lungs and BAL fluid cells and BAL fluid of Grx1 KO compared to wild type mice exposed to cigarette smoke and air.

<p>S-glutathionylation of proteins quantified by the DTNB assay in lung tissue of Grx1 KO compared to wild type mice exposed to cigarette smoke and air, data shown in nmol GSH/mg protein and represented as mean ± SD (A). S-glutathionylation of proteins visualised by the biotin switch staining in BAL fluid cells of Grx1 KO compared to wild type mice exposed to cigarette smoke and air, the outer right panel shows the quantification of staining expressed as relative fluorescent intensity of red staining (S-glutathionylated proteins) compared to blue, nuclear DAPI staining and represented as mean ± SD (B). S-glutathionylation of proteins quantified by the DTNB assay in BAL fluid of Grx1 KO compared to wild type mice exposed to cigarette smoke and air, data shown in nmol GSH/µg protein and represented as mean ± SD (C). * represents p<0.05 between air and smoke exposed mice, # represents p<0.05 between WT smoke and KO smoke.</p

Lung tissue differential cell counts in wild type versus Grx1 KO mice exposed to air or smoke.

<p>Total lung cells (A), total numbers of lung macrophages (B), dendritic cells (C), CD3+ cells (D), CD4+ CD69+ cells (E) CD8+ CD69+ cells (F) and GR1 cells (G), represented as mean ± SD. * represents p<0.05 between air and smoke exposed mice.</p

KC in primary macrophages versus primary epithelial cells from wild type and Grx1 KO mice after cigarette smoke extract exposure.

<p>KC in medium of primary macrophages (A) and of mouse tracheal epithelial cells (B) from wild type and Grx1 KO mice exposed to air or 0.1% cigarette smoke extract for 24 hours expressed in pg/ml.</p