Ct values generated from FMDV 3D rRT-PCR performed on sections of 5 separate LFDs and MagNA pure extracted RNA from the original epithelial suspensions and recorded by time of LFD testing.

<p>LP: Loading Pad; WS: Wicking strip; NB: Nitrocellulose below Ab Band (NB); Nitrocellulose Ab Band (AbB); Nitrocellulose above Ab band (NA). LFDs spanned two serotypes O (red dots) (LFDs HKN 10/2005, UKG 7B/2007) and A (blue dots) (LFDs TUR 20/2006, IRN53/2006, IRN 36/2007).</p

Ct values generated from FMDV 3D rRT-PCR performed on sections of 14 separate LFDs and corresponding values generated for MagNA pure extracted RNA from the equivalent original epithelial suspensions in parallel.

<p>LP: Loading Pad; WS: Wicking strip; NB: Nitrocellulose below Ab Band (NB); Nitrocellulose Ab Band (AbB); Nitrocellulose above Ab band (NA). LFDs spanned four serotypes O (red dots) (LFDs TUR 8/1969, BAR 2/2008, KUW 2/2008, SAU 3/2008, ZAM 5/2008, HKN 10/2005, IRN 53/2006, UKG 7B/2007, A (blue dots) (LFDs BAR 4/2009, IRN 1/2008, KEN 8/2008, TUR 20/2006, IRN 36/2007), Asia 1 (grey dots) (IRN 15/2001) and SAT 1 (yellow dots) (ZAM 5/2008).</p

Ct values generated from FMDV 3D rRT-PCR performed on sections of LFDs in dilution series from serotype O isolate (BAR 2/2008) compared against the visual detection on the Ag LFD.

<p>LP: Loading Pad; WS: Wicking strip; Nitrocellulose Ab Band (AbB)(+)  =  genome detected; (-)  =  No Ct.</p

Visualization of the 24 overlapping PCR products representing the complete FMDV genome generated from the loading pad (LP) from LFD loaded with UKG 7B/2007.

<p>1.8% agarose gel stained with ethidium bromide (0.2 µg per ml). Lane 1, S-fragment of the 5′UTR, Lanes 2–22 L fragment, Lanes 23 and 24, poly A fragment.</p

Antigen (Ag) ELISA results from electroporated BHK cells passed once and twice on BTY cells.

<p>LP: Loading Pad; WS: Wicking strip; NC: Nitrocellulose. +: Obvious CPE; +/-: Suspected CPE: -: No CPE. O  =  O serotype detected, A  =  A serotype detected, Asia 1  =  Asia 1 serotype detected. LP/WS: Loading Pad combined with Wicking Strip; NC: Nitrocellulose. (1)  =  LFDs washed the same day. (2)  =  LFDs washed one week later.</p><p>Antigen (Ag) ELISA results from electroporated BHK cells passed once and twice on BTY cells.</p

Box plot of Ct values generated from FMDV 3D rRT-PCR performed on sections of LFDs from serotype SAT 1 isolate (ZAM 5/2008) eluted at one month after LFD testing.

<p>LFDs had either been stored at room temperature (RoomT) (green bar) or 37°C (red bar). LP: Loading Pad; WS: Wicking strip; Nitrocellulose Ab Band (AbB). Dotted blue line represents baseline data (Ct values from LFDs washed on day one).</p

Foot-and-mouth disease virus samples used in the study.

<p>ES: Tongue epithelial suspension. CC: Cell culture supernatant.</p><p>Foot-and-mouth disease virus samples used in the study.</p

Number of investigated and positive herds obtained in each country by the different partners, in the three 12-month periods of the ESNIP3 program as well as in total from November 2010 to October 2013.

<p>Percentages of positive herds and total numbers of viruses that were subtyped in the context of the passive surveillance are also given. When several partners were involved in the surveillance in a country, individual numbers are indicated in grey.</p><p>Number of investigated and positive herds obtained in each country by the different partners, in the three 12-month periods of the ESNIP3 program as well as in total from November 2010 to October 2013.</p

Number of viruses identified within the different haemagglutinin and neuraminidase subtypes and lineages in 14 countries from November 2010 to October 2013.

<p>Number of viruses identified within the different haemagglutinin and neuraminidase subtypes and lineages in 14 countries from November 2010 to October 2013.</p

Overview of RT-PCR techniques and pig sera used by 15 ESNIP3 partners for detection and preliminary subtyping of influenza A viruses in pig samples (rt = real-time; conv. = conventional; n.a. = not applicable; CA = Cotes d'Armor; Fin = Finistere; Eng = England; Calif = California; Scot = Scotland; Neth = Netherlands; It = Italy).

<p>Virus strains marked with an asterisk (*) were used to produce sera belonging to the ESNIP3 reference panel.</p>a<p>Since end 2012 (used during the third yearly period of the project).</p><p>Overview of RT-PCR techniques and pig sera used by 15 ESNIP3 partners for detection and preliminary subtyping of influenza A viruses in pig samples (rt =  real-time; conv. =  conventional; n.a. =  not applicable; CA =  Cotes d'Armor; Fin = Finistere; Eng =  England; Calif =  California; Scot =  Scotland; Neth =  Netherlands; It = Italy).</p