Experimental design.

<p>TAA = thioacetamide; ip = intraperitoneal injection.</p

Representative histology of Sirius red stained liver sections from experiment described in <b>Figure 4</b>.

<p>A photomicrograph was chosen from each experimental group that was approximately equal to the mean of the group for percent area stained with Sirius red. N = nodule; Closed arrows = incomplete (broken) strands of bridging fibrosis.</p

Representative histology of Sirius red stained liver sections from experiment described in <b>Figure 1</b>.

<p>A photomicrograph was chosen from each experimental group that was approximately equal to the mean of the group for percent area stained with Sirius red. Open arrows = strands of bridging fibrosis; Closed arrows = incomplete (broken) strands of bridging fibrosis.</p

Experimental design.

<p>TAA = thioacetamide; ip = intraperitoneal injection.</p

Portal pressures in rats at sacrifice.

<p>P values compared to vehicle control (control 0.9% NaCl).</p>*<p>p<0.05;</p>**<p>p<0.001.</p

Graphical representation of the percentage Sirius red positive tissue from experiment described in <b>Figure 4</b>.

<p>Statistical analysis performed was One Way ANOVA followed by Dunnett’s multiple comparison testing to compare each group separately to group 1. Mean values, standard deviation, and adjusted p values are shown.</p

Evaluation of LX-2 cells for apoptosis.

<p>A. Fluorescent activated cell sorting following treatment with Annexin V apoptosis detection kit APC (Ebioscience). B. 2% agarose gel, stained with ethidium bromide, and visualized by transillumination with UV light after using Apoptotic DNA Ladder Extraction Kit (BioVision, Mountain View. Samples were all analyzed on same gel; discontinuity on the figure is due to the removal of repeated samples of different lots of GM-CT-01 which gave same results.</p

Western blot analysis of protein isolated from livers from animals in the experiment described in <b>Figure 4</b>.

<p>COL1 = collagen type 1; α-SMA = alpha smooth muscle actin; C = normal rat liver protein; β-tubulin was used as an internal control.</p

Histological analysis of liver sections from groups 1(V), 4 (GR) and 7 (GM) from experiment described in <b>Figure 4</b>.

<p>Liver histology from each animal was evaluated in a blinded fashion by an experienced pathologist as described in Materials and Methods. Statistical analysis was done using Mann-Whitney test for non-parametric measurements and the graph show median with interquartile range. <b>A</b>: Ishak Score; B: Portal Inflammation; C: Ballooning degeneration of hepatocytes.</p

mRNA expression in LX-2 cells after 48 hours of culture.

<p>Drug concentrations were 0.1/ml culture media. Data are expressed as mean and standard deviation. Statistics performed with t-tests as compared to control.</p