A review of Multi-Agent Simulation Models in Agriculture

Multi-Agent Simulation (MAS) models are intended to capture emergent properties of complex systems that are not amenable to equilibrium analysis. They are beginning to see some use for analysing agricultural systems. The paper reports on work in progress to create a MAS for specific sectors in New Zealand agriculture. One part of the paper focuses on options for modelling land and other resources such as water, labour and capital in this model, as well as markets for exchanging resources and commodities. A second part considers options for modelling agent heterogeneity, especially risk preferences of farmers, and the impacts on decision-making. The final section outlines the MAS that the authors will be constructing over the next few years and the types of research questions that the model will help investigate.multi-agent simulation models, modelling, agent-based model, cellular automata, decision-making, Crop Production/Industries, Environmental Economics and Policy, Farm Management, Land Economics/Use, Livestock Production/Industries,

Diurnal Urinary Excretion of DHP in Steers Fed \u3cem\u3eLeucaena leucocephala\u3c/em\u3e

Leucaena (Leucaena leucocephala) contains the toxin mimosine which is quickly degraded by rumen microorganisms to isomers of dihydroxypyridine (DHP). DHP is detrimental to animal production, causing reduced thyroid hormones, reduced weight gain, goiter and severe deficiencies in essential minerals (Tsai and Ling 1971; Hammond 1995). There are several methods of testing for exposure to DHP toxicity but the simplest is the colorimetric urine spot test (Graham et al. 2013). Several researchers have noted high variability in the excretion of DHP among animals on similar leucaena diets (Dalzell et al. 2012; Phaikaew et al. 2012) and even in the same animal over sequential samplings (O\u27Reagain and Shelton 2013). They noted that it was possible to obtain samples with very low DHP in unprotected animals on high leucaena diets, leading to the false conclusion that the animal was successfully degrading DHP in the rumen. This study examined the extent and possible causes of variation of DHP concentration in spot urine samples taken over a 6-week period, including an intensive sampling over a 24 hour period

The Growth Response of Tropical and Sub-Tropical Forage Species to Increasing Salinity

There is currently a growing coal seam gas (CSG) industry in Queensland, Australia. The industry requires beneficial-use strategies to consume the significant volumes of water released during CSG extraction. Irrigation of tropical and sub-tropical forage species for beef production is one option, however coal seam (CS) water is of varying quality due to moderate to high salinity and alkalinity. The application of chemically amended CS water over time could potentially increase soil salinity, which is known to reduce plant biomass production. While there were studies of salinity tolerance of many tropical and sub-tropical forage species 30 years ago, there is a need to examine the tolerance of more recently released species and cultivars which are suitable for planting in the Queensland CSG area

The Efficacy of \u3cem\u3ein vitro Synergistes jonesii\u3c/em\u3e Inoculum in Preventing DHP Toxicity in Steers Fed Leucaena-Grass Diets

Leucaena leucocephala (leucaena) is a valuable forage tree legume for tropical animal production that contains the toxin mimosine. The breakdown products of mimosine in ruminants (3,4-DHP and 2,3-DHP) can adversely affect their health and limit weight gains (Jones and Hegarty 1984). The rumen bacterium Synergistes jonesii, introduced into Australia in 1983 was shown to completely and rapidly degrade these toxins to safe levels (Jones and Megarrity 1986). Since 1996, an in vitro produced inoculum has been made commercially available to Australian graziers (Klieve et al. 2002). Accordingly, the issue of leucaena toxicity in Australia was thought to be resolved. However, extensive testing in 2004 found that up to 50% of Queensland cattle herds consuming leucaena were excreting high levels of urinary DHP suggesting sub-clinical toxicity remained an issue for graziers (Dalzell et al. 2012). Some of these herds had previously been inoculated with in vitro S. jonesii suggesting the inoculum may not be able to either persist within a herd, or remain effective in degrading DHP. The aim of this study was to assess the capability of the in vitro S. jonesii inoculum to efficiently break down DHP in a controlled feeding trial environment

Relationship between body composition, inflammation and lung function in overweight and obese asthma

Background: The obese-asthma phenotype is not well defined. The aim of this study was to examine both mechanical and inflammatory influences, by comparing lung function with body composition and airway inflammation in overweight and obese asthma. Methods: Overweight and obese (BMI 28-40 kg/m2) adults with asthma (n = 44) completed lung function assessment and underwent full-body dual energy x-ray absorptiometry. Venous blood samples and induced sputum were analysed for inflammatory markers. Results: In females, android and thoracic fat tissue and total body lean tissue were inversely correlated with expiratory reserve volume (ERV). Conversely in males, fat tissue was not correlated with lung function, however there was a positive association between android and thoracic lean tissue and ERV. Lower body (gynoid and leg) lean tissue was positively associated with sputum %neutrophils in females, while leptin was positively associated with android and thoracic fat tissue in males. Conclusions: This study suggests that both body composition and inflammation independently affect lung function, with distinct differences between males and females. Lean tissue exacerbates the obese-asthma phenotype in females and the mechanism responsible for this finding warrants further investigation

Loss of N-WASP drives early progression in an Apc model of intestinal tumourigenesis

N‐WASP (WASL) is a widely expressed cytoskeletal signalling and scaffold protein also implicated in regulation of Wnt signalling and homeostatic maintenance of skin epithelial architecture. N‐WASP mediates invasion of cancer cells in vitro and its depletion reduces invasion and metastatic dissemination of breast cancer. Given this role in cancer invasion and universal expression in the gastrointestinal tract, we explored a role for N‐WASP in the initiation and progression of colorectal cancer. While deletion of N‐wasp is not detectably harmful in the murine intestinal tract, numbers of Paneth cells increased, indicating potential changes in the stem cell niche and migration up the crypt‐villus axis was enhanced. Loss of N‐wasp promoted adenoma formation in an adenomatous polyposis coli (Apc) deletion model of intestinal tumourigenesis. Thus, we establish a tumour suppressive role of N‐WASP in early intestinal carcinogenesis despite its later pro‐invasive role in other cancers. Our study highlights that while the actin cytoskeletal machinery promotes invasion of cancer cells, it also maintains normal epithelial tissue function and thus may have tumour suppressive roles in pre‐neoplastic tissues

Effects of Monobutyl and Di(n-butyl) Phthalate in Vitro on Steroidogenesis and Leydig Cell Aggregation in Fetal Testis Explants from the Rat: Comparison with Effects in Vivo in the Fetal Rat and Neonatal Marmoset and in Vitro in the Human

BACKGROUND: Certain phthalates can impair Leydig cell distribution and steroidogenesis in the fetal rat in utero, but it is unknown whether similar effects might occur in the human. OBJECTIVES: Our aim in this study was to investigate the effects of di(n-butyl) phthalate (DBP), or its metabolite monobutyl phthalate (MBP), on testosterone production and Leydig cell aggregation (LCA) in fetal testis explants from the rat and human, and to compare the results with in vivo findings for DBP-exposed rats. We also wanted to determine if DBP/MBP affects testosterone production in vivo in the neonatal male marmoset. METHODS: Fetal testis explants obtained from the rat [gestation day (GD)19.5] and from the human (15–19 weeks of gestation) were cultured for 24–48 hr with or without human chorionic gonadotropin (hCG) or 22R-hydroxycholesterol (22R-OH), and with or without DBP/MBP. Pregnant rats and neonatal male marmosets were dosed with 500 mg/kg/day DBP or MBP. RESULTS: Exposure of rats in utero to DBP (500 mg/kg/day) for 48 hr before GD21.5 induced major suppression of intratesticular testosterone levels and cytochrome P450 side chain cleavage enzyme (P450scc) expression; this short-term treatment induced LCA, but was less marked than longer term (GD13.5–20.5) DBP treatment. In vitro, MBP (10(−3) M) did not affect basal or 22R-OH-stimulated testosterone production by fetal rat testis explants but slightly attenuated hCG-stimulated steroidogenesis; MBP induced minor LCA in vitro. None of these parameters were affected in human fetal testis explants cultured with 10(−3) M MBP for up to 48 hr. Because the in vivo effects of DBP/MBP were not reproduced in vitro in the rat, the absence of MBP effects in vitro on fetal human testes is inconclusive. In newborn (Day 2–7) marmosets, administration of a single dose of 500 mg/kg MBP significantly (p = 0.019) suppressed blood testosterone levels 5 hr later. Similar treatment of newborn co-twin male marmosets for 14 days resulted in increased Leydig cell volume per testis (p = 0.011), compared with co-twin controls; this is consistent with MBP-induced inhibition of steroidogenesis followed by compensatory Leydig cell hyperplasia/hypertrophy. CONCLUSIONS: These findings suggest that MBP/DBP suppresses steroidogenesis by fetal-type Leydig cells in primates as in rodents, but this cannot be studied in vitro

Legionella pneumophila strain 130b possesses a unique combination of type IV secretion systems and novel Dot/Icm secretion system effector proteins

Legionella pneumophila is a ubiquitous inhabitant of environmental water reservoirs. The bacteria infect a wide variety of protozoa and, after accidental inhalation, human alveolar macrophages, which can lead to severe pneumonia. The capability to thrive in phagocytic hosts is dependent on the Dot/Icm type IV secretion system (T4SS), which translocates multiple effector proteins into the host cell. In this study, we determined the draft genome sequence of L. pneumophila strain 130b (Wadsworth). We found that the 130b genome encodes a unique set of T4SSs, namely, the Dot/Icm T4SS, a Trb-1-like T4SS, and two Lvh T4SS gene clusters. Sequence analysis substantiated that a core set of 107 Dot/Icm T4SS effectors was conserved among the sequenced L. pneumophila strains Philadelphia-1, Lens, Paris, Corby, Alcoy, and 130b. We also identified new effector candidates and validated the translocation of 10 novel Dot/Icm T4SS effectors that are not present in L. pneumophila strain Philadelphia-1. We examined the prevalence of the new effector genes among 87 environmental and clinical L. pneumophila isolates. Five of the new effectors were identified in 34 to 62% of the isolates, while less than 15% of the strains tested positive for the other five genes. Collectively, our data show that the core set of conserved Dot/Icm T4SS effector proteins is supplemented by a variable repertoire of accessory effectors that may partly account for differences in the virulences and prevalences of particular L. pneumophila strains. Copyright © 2010, American Society for Microbiology. All Rights Reserved

Recurrent Tissue-Specific Mtdna Mutations are Common in Humans

Mitochondrial DNA (mtDNA) variation can affect phenotypic variation; therefore, knowing its distribution within and among individuals is of importance to understanding many human diseases. Intra-individual mtDNA variation (heteroplasmy) has been generally assumed to be random. We used massively parallel sequencing to assess heteroplasmy across ten tissues and demonstrate that in unrelated individuals there are tissue-specific, recurrent mutations. Certain tissues, notably kidney, liver and skeletal muscle, displayed the identical recurrent mutations that were undetectable in other tissues in the same individuals. Using RFLP analyses we validated one of the tissue-specific mutations in the two sequenced individuals and replicated the patterns in two additional individuals. These recurrent mutations all occur within or in very close proximity to sites that regulate mtDNA replication, strongly implying that these variations alter the replication dynamics of the mutated mtDNA genome. These recurrent variants are all independent of each other and do not occur in the mtDNA coding regions. The most parsimonious explanation of the data is that these frequently repeated mutations experience tissue-specific positive selection, probably through replication advantage

Life\u27s Essential 8: Optimizing Health in Older Adults

The population worldwide is getting older as a result of advances in public health, medicine, and technology. Older individuals are living longer with a higher prevalence of subclinical and clinical cardiovascular disease (CVD). In 2010, the American Heart Association introduced a list of key prevention targets, known as Life\u27s Simple 7 to increase CVD-free survival, longevity, and quality of life. In 2022, sleep health was added to expand the recommendations to Life\u27s Essential 8 (eat better, be more active, stop smoking, get adequate sleep, manage weight, manage cholesterol, manage blood pressure, and manage diabetes). These prevention targets are intended to apply regardless of chronologic age. During this same time, the understanding of aging biology and goals of care for older adults further enhanced the relevance of prevention across the range of functions. From a biological perspective, aging is a complex cellular process characterized by genomic instability, telomere attrition, loss of proteostasis, inflammation, deregulated nutrient-sensing, mitochondrial dysfunction, cellular senescence, stem cell exhaustion, and altered intercellular communication. These aging hallmarks are triggered by and enhanced by traditional CVD risk factors leading to geriatric syndromes (eg, frailty, sarcopenia, functional limitation, and cognitive impairment) which complicate efforts toward prevention. Therefore, we review Life\u27s Essential 8 through the lens of aging biology, geroscience, and geriatric precepts to guide clinicians taking care of older adults