809 research outputs found
Evaluation of exercises designed to improve children's use of descriptive words in oral language
Full text linkThesis (Ed.M.)--Boston Universit
A pan-cetacean MHC amplicon sequencing panel developed and evaluated in combination with genome assemblies
Get PDFThis study was funded by a Royal Society Research Grants for Research Fellows (RGF\R1\181014) to E.C.G. E.C.G. is funded by a Royal Society University Research Fellowship (UF160081 & URF\R\221020). F.E. is supported by a University of St Andrews School of Biology Ph.D. scholarship and a Royal Society Research Fellows Enhancement Award (RGF\EA\180213 to E.C.G). E.L.C. is funded by a Rutherford Discovery Fellowship from the Royal Society of New Zealand Te Apārangi.The major histocompatibility complex (MHC) is a highly polymorphic gene family that is crucial in immunity, and its diversity can be effectively used as a fitness marker for populations. Despite this, MHC remains poorly characterised in non-model species (e.g., cetaceans: whales, dolphins and porpoises) as high gene copy number variation, especially in the fast-evolving class I region, makes analyses of genomic sequences difficult. To date, only small sections of class I and IIa genes have been used to assess functional diversity in cetacean populations. Here, we undertook a systematic characterisation of the MHC class I and IIa regions in available cetacean genomes. We extracted full-length gene sequences to design pan-cetacean primers that amplified the complete exon2 from MHC class I and IIa genes in one combined sequencing panel. We validated this panel in 19 cetacean species and described 354 alleles for both classes. Furthermore, we identified likely assembly artefacts for many MHC class I assemblies based on the presence of class I genes in the amplicon data compared to missing genes from genomes. Finally, we investigated MHC diversity using the panel in 25 humpback and 30 southern right whales, including four paternity trios for humpback whales. This revealed copy-number variable class I haplotypes in humpback whales, which is likely a common phenomenon across cetaceans. These MHC alleles will form the basis for a cetacean branch of the Immuno-Polymorphism Database (IPD-MHC), a curated resource intended to aid in the systematic compilation of MHC alleles across several species, to support conservation initiatives.Peer reviewe
Research for Equity and Power (REP): Strategies and Lessons Learned about Community-based Participatory Research (CBPR)
Get PDFFor the past two years, Homewood Children’s Village and the University of Pittsburgh School of Social Work have worked alongside one another and with community residents to increase civic engagement around positive community change. This workshop illustrated how CBPR principles have furthered the community engaged partnership, and included challenges and lessons learned
Neutron Position Sensitive Detectors for the ESS
Full text linkThe European Spallation Source (ESS) in Lund, Sweden will become the world's
leading neutron source for the study of materials. The instruments are being
selected from conceptual proposals submitted by groups from around Europe.
These instruments present numerous challenges for detector technology in the
absence of the availability of Helium-3, which is the default choice for
detectors for instruments built until today and due to the extreme rates
expected across the ESS instrument suite. Additionally a new generation of
source requires a new generation of detector technologies to fully exploit the
opportunities that this source provides. The detectors will be sourced from
partners across Europe through numerous in-kind arrangements; a process that is
somewhat novel for the neutron scattering community. This contribution presents
briefly the current status of detectors for the ESS, and outlines the timeline
to completion. For a conjectured instrument suite based upon instruments
recommended for construction, a recently updated snapshot of the current
expected detector requirements is presented. A strategy outline as to how these
requirements might be tackled by novel detector developments is shown. In terms
of future developments for the neutron community, synergies should be sought
with other disciples, as recognized by various recent initiatives in Europe, in
the context of the fundamentally multi-disciplinary nature of detectors. This
strategy has at its basis the in-kind and collaborative partnerships necessary
to be able to produce optimally performant detectors that allow the ESS
instruments to be world-leading. This foresees and encourages a high level of
collaboration and interdependence at its core, and rather than each group being
all-rounders in every technology, the further development of centres of
excellence across Europe for particular technologies and niches.Comment: 8 pages, 1 figure. Proceedings from the 23rd International Workshop
on Vertex Detectors, 15-19 September 2014, Macha Lake, The Czech Republic.
PoS(Vertex2014)02
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