46 research outputs found
Micro-heterogeneity of human saliva peptide P-C characterized by high-resolution top-down fourier-transform mass spectrometry
Full text linkChemInform Abstract: SPALTUNG EINZELNER AMINOSAEURE-RESTE VOM MERRIFIELD-HARZ DURCH HCL UND HF
No full textTFA/HCl mixture as an alternative to the standard propionic acid/HCl mixture for aminoacyl- and peptidyl-resin hydrolysis
No full textSynthesis of cross-linked polystyrene-supported polyethylene glycol and investigation of its property in peptide synthesis
No full textFirst evidence of spontaneous deamidation of glutamine residue via cyclic imide to α- and γ-glutamic residue under physiological conditions
No full textA new micro-test for the detection of incomplete coupling reactions in solid-phase peptide synthesis using 2,4,6-trinitrobenzene-sulphonic acid
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Protein deamidation
Get PDFA completely automatic computerized technique for the quantitative estimation of the deamidation rates of any protein for which the three-dimensional structure is known has been developed. Calculations of the specific deamidation rates of 170,014 asparaginyl residues in 13,335 proteins have been carried out. The calculated values have good quantitative reliability when compared with experimental measurements. These rates demonstrate that deamidation may be a biologically relevant phenomenon in a remarkably large percentage of proteins
