Purge–Mainstream Interactions in a Turbine Stage With Rotor Endwall Contouring

Purge flows are prevalent in modern gas turbine design, allowing for increased turbine entry temperatures. The purge flow passes through a rim seal and interacts with the mainstream flow, modifying the blade secondary flow structures and reducing stage efficiency. These structures may be controlled using end wall contouring (EWC), though experimental demonstration of their benefit is seldom reported in the literature. The optically accessible turbine at the University of Bath was designed to directly measure and visualize the flow field within the blade passage for a rotor with EWC. The single-stage turbine enables phase-locked flow field measurements with volumetric particle image velocimetry (PIV). Purge flow was supplied to investigate a range of operating conditions in which the secondary flow structures were modified. The modular turbine rotor allowed for expedient change of a bladed ring, or bling, featuring non-axisymmetric EWC. The identified secondary flow structures were the pressure-side leg of the horse shoe vortex (PS-HSV) and an egress vortex (EV) of concurrent rotational direction. An increase in purge flowrate monotonically shifted the EV toward the suction-side (SS) of the adjacent blade. The migration of the PS-HSV toward the SS caused the two aforementioned vortices to merge. The EWC rotor design included a leading-edge (LE) feature to alter the PS-HSV and a trough to guide the EV low spanwise in the passage and maintain displacement from the adjacent suction-side. The EWC rotor was found to be effective at altering the formation and positioning of the secondary flow structures at a range of purge flow conditions

Assessing recombinant vaccinia virus as a delivery system for fertility control vaccines in the brushtail possum (Trichosurus vulpecula)

Duckworth, J., Cross, M., Fleming, S., Scobie, S., Whelan, E., Prada, D., Mercer, A., Cowan, P

Anthrax Toxin Receptor 2 Determinants that Dictate the pH Threshold of Toxin Pore Formation

The anthrax toxin receptors, ANTXR1 and ANTXR2, act as molecular clamps to prevent the protective antigen (PA) toxin subunit from forming pores until exposure to low pH. PA forms pores at pH ∼6.0 or below when it is bound to ANTXR1, but only at pH ∼5.0 or below when it is bound to ANTXR2. Here, structure-based mutagenesis was used to identify non-conserved ANTXR2 residues responsible for this striking 1.0 pH unit difference in pH threshold. Residues conserved between ANTXR2 and ANTXR1 that influence the ANTXR2-associated pH threshold of pore formation were also identified. All of these residues contact either PA domain 2 or the neighboring edge of PA domain 4. These results provide genetic evidence for receptor release of these regions of PA as being necessary for the protein rearrangements that accompany anthrax toxin pore formation

Sheep Updates 2003 - Husbandry

This session covers seven papers from different authors:1. Setting up a successful, low input feedlot Paul Barrett, ‘Bimberdong’ Jerramungup 2. Effective mineral supplementation of sheep Kevin Bell, Sheep Management and Production Consultants, Kojonup, WA 3. Genetic benchmarking for WA sheep producers J. Greeff, L. Butler, S. Brown, K. Hart and A. Gray Department of Agriculture Western Australia 4. Does selecting sheep for low WEC reduce scouring? John Karlsson, Johan Greeff and Paula Coombe, Department of Agriculture Western Australia 5. Summer quarters for sheep - stubbles Ron McTaggart, Department of Agriculture Western Australia, Albany 6. Thinking about breeding Easy Care Sheep? David Scobie, AgResearch PO Box 60 Lincoln, 8152, New Zealand 7. Increasing lambing percentages and lamb survival Sandy White, Department of Agriculture Western Australia, Jerramungu

BAZ1B in Nucleus Accumbens Regulates Reward-Related Behaviors in Response to Distinct Emotional Stimuli

ATP-dependent chromatin remodeling proteins are being implicated increasingly in the regulation of complex behaviors, including models of several psychiatric disorders. Here, we demonstrate that Baz1b, an accessory subunit of the ISWI family of chromatin remodeling complexes, is upregulated in the nucleus accumbens (NAc), a key brain reward region, in both chronic cocaine-treated mice and mice that are resilient to chronic social defeat stress. In contrast, no regulation is seen in mice that are susceptible to this chronic stress. Viral-mediated overexpression of Baz1b, along with its associated subunit Smarca5, in mouse NAc is sufficient to potentiate both rewarding responses to cocaine, including cocaine self-administration, and resilience to chronic social defeat stress. However, despite these similar, proreward behavioral effects, genome-wide mapping of BAZ1B in NAc revealed mostly distinct subsets of genes regulated by these chromatin remodeling proteins after chronic exposure to either cocaine or social stress. Together, these findings suggest important roles for BAZ1B and its associated chromatin remodeling complexes in NAc in the regulation of reward behaviors to distinct emotional stimuli and highlight the stimulus-specific nature of the actions of these regulatory proteins