Lesiones patológicas inusuales en una vaquillona afectada por Trueperella pyogenes

Four Hereford heifers of approximately 18 months of age presented nodular formations in the uterus detected by transrectal palpation. This pathology had been previously diagnosed in the herd, which led to the early discarding of 1-2% of heifers per year. Post mortem examination in a severely affected heifer revealed multiple abscesses in the pelvic and abdominal cavity associated with the uterus, serosa of the forelimbs, and liver. Microscopically, the abscesses were characterized by a necrotic center with viable and degenerated neutrophils and necrotic debris, surrounded by a well-defined wall of connective tissue. In addition, multifocal neutrophilic ruminitis associated with acidosis was observed. Intralesional gram-positive bacilli were detected in the abscesses. Trueperella pyogenes was isolated from the purulent content. It is assumed that rumen acidosis was the origin of the dissemination and abscess formation of T. pyogenes in various tissues, including the uterus.Cuatro vaquillonas Hereford de aproximadamente 18 meses de edad presentaron formaciones nodulares en el útero detectadas por palpación transrectal. Esta patología había sido previamente diagnosticada en el rodeo, lo que llevó al descarte temprano de 1-2% de vaquillonas por año. El examen post mortem en una vaquillona gravemente afectada reveló múltiples abscesos en la cavidad pélvica y abdominal asociados al útero, serosa de los preestómagos y el hígado. Microscópicamente, los abscesos se caracterizaron por un centro necrótico con neutrófilos viables y degenerados y restos necróticos, rodeados por una pared bien definida de tejido conectivo. Además, se observó ruminitis neutrofílica multifocal asociada a acidosis. Se detectaron bacilos grampositivos intralesionales en los abscesos. Del contenido purulento se aisló Trueperella pyogenes. Se asume que la acidosis ruminal fue el origen de la diseminación y formación de abscesos de T. pyogenes en varios tejidos, incluido el útero

Controlling Endemic Neospora caninum-Related Abortions in a Dairy Herd From Argentina

After diagnosis of endemic abortions due to neosporosis in a commercial dairy farm, routes of Neospora caninum-transmission were evaluated in order to choose the best strategy for reducing its seroprevalence and related abortions. Fifty two dam-calf pairs were bled at parturition. Additionally, 22 female calves were also sampled at regular 3 month intervals until 18–22 months. N. caninum specific antibodies were assayed by IFAT. Serum samples were tested at a dilution 1:25 for calves before colostrum intake and heifers before mating and 1:100 for multiparous cows. Only serum samples from IFAT seropositive cattle involved in the evaluation of the routes of transmission were assessed by a commercial IgG avidity ELISA. Seropositive cows or heifers were artificially inseminated with semen from Hereford bulls. The progenies from these female animals were sent to a feed lot to produce meat. Different generalized linear models (GLM) were used to study the relationship between abortion, age category, and serostatus. Seropositive heifers were more likely to have a record of abortion (OR 2.7; 95% CI 1.6–4.7). Vertical transmission frequency was 55.5% (5 seropositive calves/9 seropositive cows). Horizontal transmission was 22.7% (5 female calves seroconverted at least one time/22 females calves sampled during 24 months) and these 5 female calves had low avidity. In heifers, both seroprevalence and abortion rates decreased from 22.1 and 8.4% of 475 in 2009 to 6.1 and 4.3% of 578 in 2015, respectively (p < 0.01). Over 5 years, N. caninum-seroprevalence and the related abortions in heifers decreased after the control strategy was assessed.Fil: Lagomarsino, H.. No especifíca;Fil: Scioli, Agustín. No especifíca;Fil: Rodríguez, Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Armendano, Joaquín Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Fiorani, Franco. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Bence, Ángel. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: García, Joaquín. No especifíca;Fil: Hecker, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Cantón, Germán. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Odeón, Anselmo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Campero, Carlos Manuel. No especifíca;Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentin

Controlling endemic Neospora caninum-related abortions in a dairy herd from Argentina

After diagnosis of endemic abortions due to neosporosis in a commercial dairy farm, routes of Neospora caninum-transmission were evaluated in order to choose the best strategy for reducing its seroprevalence and related abortions. Fifty two dam-calf pairs were bled at parturition. Additionally, 22 female calves were also sampled at regular 3 month intervals until 18–22 months. N. caninum specific antibodies were assayed by IFAT. Serum samples were tested at a dilution 1:25 for calves before colostrum intake and heifers before mating and 1:100 for multiparous cows. Only serum samples from IFAT seropositive cattle involved in the evaluation of the routes of transmission were assessed by a commercial IgG avidity ELISA. Seropositive cows or heifers were artificially inseminated with semen from Hereford bulls. The progenies from these female animals were sent to a feed lot to produce meat. Different generalized linear models (GLM) were used to study the relationship between abortion, age category, and serostatus. Seropositive heifers were more likely to have a record of abortion (OR 2.7; 95% CI 1.6–4.7). Vertical transmission frequency was 55.5% (5 seropositive calves/9 seropositive cows). Horizontal transmission was 22.7% (5 female calves seroconverted at least one time/22 females calves sampled during 24 months) and these 5 female calves had low avidity. In heifers, both seroprevalence and abortion rates decreased from 22.1 and 8.4% of 475 in 2009 to 6.1 and 4.3% of 578 in 2015, respectively (p < 0.01). Over 5 years, N. caninum-seroprevalence and the related abortions in heifers decreased after the control strategy was assessed.EEA BalcarceFil: Lagomarsino, Horacio. Actividad privada; ArgentinaFil: Scioli, Agustín. Actividad privada; ArgentinaFil: Rodríguez, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Armendano, Joaquín. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Fiorani, Franco. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Bence, Ángel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.Fil: García, Joaquín. Actividad privada; ArgentinaFil: Hecker, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Cantón, Germán. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce, ArgentinaFil: Campero, Carlos. Actividad privada; ArgentinaFil: Moore, Dadín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina