Role of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) in Modulating Vascular Smooth Muscle Cells by Activating Large-Conductance Potassium Ion Channels

International audienceIn this chapter we propose to discuss the role of K+ ion channels in stimulating vasodilatation by altering the membrane potential of vascular smooth muscle cells. We present evidence that the K+ channels are modulated by a direct action of non-steroidal antiinflammatory drugs (NSAIDs) to activate the K+ ion channels

Characterization of the P(2) receptors in rabbit pulmonary artery

1. We have identified the P(2) receptors mediating vasomotor responses in the rabbit pulmonary artery. 2. Neither ATP nor UTP contracted intact or endothelium-denuded rings. However, both relaxed intact rings of rabbit pulmonary artery that had been preconstricted with phenylephrine (pD(2) 5.2 and 5.6, respectively). 3. The vasodilator effect of UTP was endothelium-dependent and abolished by the nitric oxide synthase inhibitor N(G)-nitro-L-arginine (L-NOARG). 4. The vasodilator effect of ATP was only partially inhibited by removal of endothelium or addition of L-NOARG, suggesting an additional direct effect on vascular smooth muscle. 5. The endothelium-dependent vasodilator responses to UTP and ATP were competitively antagonized by suramin. 6. Preconstricted, endothelium-denuded rings were also relaxed by 2-methylthio ATP (pD(2) 6.6), a P(2Y) receptor agonist. 7. Ca(2+)-mobilizing P(2U) receptors were identified on smooth muscle cells on the basis of single cell responses to ATP (pD(2) 7.8) and UTP (pD(2) 7.9; 6.7 in the presence of 100 μM suramin). 8. There was no evidence of a Ca(2+)-mobilizing P(2Y) receptor in these cultured cells. 9. The data suggest the presence of (i) a suramin-sensitive P(2U) receptor on endothelial cells that induces vasorelaxation through NO release, (ii) a suramin-sensitive P(2U) receptor on cultured smooth muscle cells that mobilizes Ca(2+) but is not coupled to vasomotor responses and (iii) a putative P(2Y) receptor on vascular smooth muscle cells that induces relaxation via a Ca(2+)-independent signal transduction pathway