Rotating bio-reactor cell culture apparatus

A bioreactor system is described in which a tubular housing contains an internal circularly disposed set of blade members and a central tubular filter all mounted for rotation about a common horizontal axis and each having independent rotational support and rotational drive mechanisms. The housing, blade members and filter preferably are driven at a constant slow speed for placing a fluid culture medium with discrete microbeads and cell cultures in a discrete spatial suspension in the housing. Replacement fluid medium is symmetrically input and fluid medium is symmetrically output from the housing where the input and the output are part of a loop providing a constant or intermittent flow of fluid medium in a closed loop

Method for culturing mammalian cells in a perfused bioreactor

A bio-reactor system wherein a tubular housing contains an internal circularly disposed set of blade members and a central tubular filter all mounted for rotation about a common horizontal axis and each having independent rotational support and rotational drive mechanisms. The housing, blade members and filter preferably are driven at a constant slow speed for placing a fluid culture medium with discrete microbeads and cell cultures in a discrete spatial suspension in the housing. Replacement fluid medium is symmetrically input and fluid medium is symmetrically output from the housing where the input and the output are part of a loop providing a constant or intermittent flow of fluid medium in a closed loop

Experimental measurement of the orbital paths of particles sedimenting within a rotating viscous fluid as influenced by gravity

Measurements were taken of the path of a simulated typical tissue segment or 'particle' within a rotating fluid as a function of gravitational strength, fluid rotation rate, particle sedimentation rate, and particle initial position. Parameters were examined within the useful range for tissue culture in the NASA rotating wall culture vessels. The particle moves along a nearly circular path through the fluid (as observed from the rotating reference frame of the fluid) at the same speed as its linear terminal sedimentation speed for the external gravitational field. This gravitationally induced motion causes an increasing deviation of the particle from its original position within the fluid for a decreased rotational rate, for a more rapidly sedimenting particle, and for an increased gravitational strength. Under low gravity conditions (less than 0.1 G), the particle's motion through the fluid and its deviation from its original position become negligible. Under unit gravity conditions, large distortions (greater than 0.25 inch) occur even for particles of slow sedimentation rate (less than 1.0 cm/sec). The particle's motion is nearly independent of the particle's initial position. Comparison with mathematically predicted particle paths show that a significant error in the mathematically predicted path occurs for large particle deviations. This results from a geometric approximation and numerically accumulating error in the mathematical technique

High aspect reactor vessel and method of use

An improved bio-reactor vessel and system useful for carrying out mammalian cell growth in suspension in a culture media are presented. The main goal of the invention is to grow and maintain cells under a homogeneous distribution under acceptable biochemical environment of gas partial pressures and nutrient levels without introducing direct agitation mechanisms or associated disruptive mechanical forces. The culture chamber rotates to maintain an even distribution of cells in suspension and minimizes the length of a gas diffusion path. The culture chamber design is presented and discussed

National Time Accounting: The Currency of Life

This monograph proposes a new approach for measuring features of society’s subjective well-being, based on time allocation and affective experience. We call this approach National Time Accounting (NTA). National Time Accounting is a set of methods for measuring, comparing and analyzing how people spend and experience their time -- across countries, over historical time, or between groups of people within a country at a given time. The approach is based on evaluated time use, or the flow of emotional experience during daily activities. After reviewing evidence on the validity of subjective well-being measures, we present and evaluate diary-based survey techniques designed to measure individuals’ emotional experiences and time use. We illustrate NTA with: (1) a new cross-sectional survey on time use and emotional experience for a representative sample of 4,000 Americans; (2) historical data on the amount of time devoted to various activities in the United States since 1965; and (3) a comparison of time use and wellbeing in the United States and France. In our applications, we focus mainly on the Uindex, a measure of the percentage of time that people spend in an unpleasant state, defined as an instance in which the most intense emotion is a negative one. The U-index helps to overcome some of the limitations of interpersonal comparisons of subjective well-being. National Time Accounting strikes us as a fertile area for future research because of advances in subjective measurement and because time use data are now regularly collected in many countries.

Three-dimensional cell to tissue assembly process

The present invention relates a 3-dimensional cell to tissue and maintenance process, more particularly to methods of culturing cells in a culture environment, either in space or in a gravity field, with minimum fluid shear stress, freedom for 3-dimensional spatial orientation of the suspended particles and localization of particles with differing or similar sedimentation properties in a similar spatial region

N=1 String Duality

We discuss duality between Type IIA string theory, eleven-dimensional supergravity, and heterotic string theory in four spacetime dimensions with $N=1$ supersymmetry. We find theories whose infrared limit is trivial at enhanced symmetry points as well as theories with $N=1$ supersymmetry but the field content of $N=4$ theories which flow to the $N=4$ fixed line in the infrared.Comment: 14 pages, harvma

Testing for double inflation with WMAP

With the WMAP data we can now begin to test realistic models of inflation involving multiple scalar fields. These naturally lead to correlated adiabatic and isocurvature (entropy) perturbations with a running spectral index. We present the first full (9 parameter) likelihood analysis of double inflation with WMAP data and find that despite the extra freedom, supersymmetric hybrid potentials are strongly constrained with less than 7% correlated isocurvature component allowed when standard priors are imposed on the cosomological parameters. As a result we also find that Akaike & Bayesian model selection criteria rather strongly prefer single-field inflation, just as equivalent analysis prefers a cosmological constant over dynamical dark energy in the late universe. It appears that simplicity is the best guide to our universe.Comment: 7 pages, 6 figure