Master of Science

thesisAdvances in silicon photonics are enabling hybrid integration of optoelectronic circuits alongside current complementary metal-oxide-semiconductor (CMOS) technologies. To fully exploit the capability of this integration, it is important to explore the effects of thermal gradients on optoelectronic devices. The sensitivity of optical components to temperature variation gives rise to design issues in silicon on insulator (SOI) optoelectronic technology. The thermo-electric effect becomes problematic with the integration of hybrid optoelectronic systems, where heat is generated from electrical components. Through the thermo-optic effect, the optical signals are in turn affected and compensation is necessary. To improve the capability of optical SOI designs, optical-wave-simulation models and the characteristic thermal operating environment need to be integrated to ensure proper operation. In order to exploit the potential for compensation by virtue of resynthesis, temperature characterization on a system level is required. Thermal characterization within the flow of physical design automation tools for hybrid optoelectronic technology enables device resynthesis and validation at a system level. Additionally, thermally-aware routing and placement would be possible. A simplified abstraction will help in the active design process, within the contemporary computer-aided design (CAD) flow when designing optoelectronic features. This thesis investigates an abstraction model to characterize the effect of a temperature gradient on optoelectronic circuit operation. To make the approach scalable, reduced order computations are desired that effectively model the effect of temperature on an optoelectronic layout; this is achieved using an electrical analogy to heat flow. Given an optoelectronic circuit, using a thermal resistance network to abstract thermal flow, we compute the temperature distribution throughout the layout. Subsequently, we show how this thermal distribution across the optoelectronic system layout can be integrated within optoelectronic device- and system-level analysis tools

Hepatic progenitor cells from adult human livers for cell transplantation.

Objective: Liver regeneration is mainly based on cellular self-renewal including progenitor cells. Efforts have been made to harness this potential for cell transplantation, but shortage of hepatocytes and premature differentiated progenitor cells from extra-hepatic organs are limiting factors. Histological studies implied that resident cells in adult liver can proliferate, have bipotential character and may be a suitable source for cell transplantation. Methods: Particular cell populations were isolated after adequate tissue dissociation. Single cell suspensions were purified by Thy-1 positivity selection, characterised in vitro and transplanted in immunodeficient Pfp/Rag2 mice. Results: Thy-1+ cells that are mainly found in the portal tract and the surrounding parenchyma, were isolated from surgical liver tissue with high yields from specimens with histological signs of regeneration. Thy-1+ cell populations were positive for progenitor (CD34, c-kit, CK14, M2PK, OV6), biliary (CK19) and hepatic (HepPar1) markers revealing their progenitor as well as hepatic and biliary nature. The potential of Thy-1+ cells for differentiation in vitro was demonstrated by increased mRNA and protein expression for hepatic (CK18, HepPar1) and biliary (CK7) markers during culture while progenitor markers CK14, chromogranin A and nestin were reduced. After transplantation of Thy-1+ cells into livers of immunodeficient mice, engraftment was predominantly seen in the periportal portion of the liver lobule. Analysis of in situ material revealed that transplanted cells express human hepatic markers HepPar1 and albumin, indicating functional engraftment. Conclusion: Bipotential progenitor cells from human adult livers can be isolated using Thy-1 and might be a potential candidate for cell treatment in liver diseases

Entwurfsmuster für verteilte Anwendungssystem-Architekturen

Aus der Motivation: "In den letzten Jahren werden verstärkt neue Organisationsformen für Unternehmen diskutiert (vgl. [Zimm97], [DaMa93], [BuIl+97]). Diese reichen von innerbetrieblicher Dezentralisierung der Entscheidungsbefugnis bis hin zur Bildung autonomer Geschäftseinheiten, die in virtuellen Unternehmen kooperieren. Neben der stärkeren Verteilung der Entscheidungsbefugnis stehen neue Formen der Arbeitsverteilung sowie die Tendenz zur Globalisierung im Vordergrund. Daraus ergeben sich weitreichende Anforderungen an betriebliche Informationssysteme. Diese betreffen insbesondere technische Merkmale wie Flexibilität und Verteilbarkeit von Anwendungssystemkomponenten sowie die Verfügbarkeit und Performanz des Gesamtsystems. Aus betriebswirtschaftlicher Sicht müssen die Zielverfolgung, die Konsistenz sowie Aspekte der Datensicherheit berücksichtigt werden (vgl. [FeSi+97]). Konventionelle Anwendungssysteme, die auf Datenintegration basieren, erfüllen insbesondere die Anforderungen nach Flexibilität und Verteilbarkeit nicht in ausreichendem Maße. Daher ist es notwendig, Anwendungssystem-Architekturen zu entwickeln, welche die Realisierung unterschiedlicher technischer und organisatorischer Verteilungsformen unterstützen. Dies erfordert einen integrierten Ansatz zur Modellierung verteilter Anwendungssysteme im Kontext organisatorischer und technischer Rahmenbedingungen. Eine geeignete Modellierungsmethode sollte somit Verteilungskonzepte für alle angesprochenen Aspekte eines betrieblichen Systems beinhalten.

DX5+NKT cells display phenotypical and functional differences between spleen and liver as well as NK1.1-Balb/c and NK1.1+ C57Bl/6 mice

These results show that DX5+NKT cells are a heterogeneous population, depending on the dedicated organ and mouse strain, that has diverse functional capacity

Increased cytoplasmatic expression of cancer immune surveillance receptor CD1d in anaplastic thyroid carcinomas

Background Anaplastic thyroid carcinomas are associated with rapid tumor growth, short survival time and without any promising therapy to improve the poor prognosis. In this study, expression of immunoregulative receptor CD1d and lymphocyte infiltration in different thyroid tumors as well as in healthy tissue were analyzed in order to find new targets for an immunotherapeutic approach. Methods CD1d immunohistochemistry was performed in samples of 18 anaplastic, 17 follicular, 27 papillary, and 4 medullary thyroid carcinomas as well as in 19 specimens from normal thyroid tissue and additionally in 10 samples of sarcoma, seven malignant melanoma and three spindle-cell lung carcinoma. Furthermore, thyroid samples were stained with antibodies against CD3, CD20, CD56, CD68, and LCA in order to analyze lymphocyte infiltration. Results For the first time CD1d receptor expression on normal thyroid tissue could be demonstrated. Moreover, anaplastic thyroid carcinomas showed significantly higher expression levels compared to other thyroid samples. Most astonishingly, CD1d expression disappeared from the cellular surface and was detected rather in the cytoplasm of anaplastic thyroid carcinoma cells. In addition, histologically similar tumors to anaplastic carcinoma like sarcoma and malignant melanoma revealed distinct CD1d staining patterns. Furthermore, infiltration of T cells, B cells, and macrophages in anaplastic thyroid carcinomas was different when compared to normal thyroid tissue and all other thyroid carcinomas. Conclusions Anaplastic thyroid carcinomas show significantly higher expression of CD1d, a receptor for NKT cells, which are subject of several anticancer therapy studies. These results may offer a novel approach to explore immunotherapeutic treatment options