Map of Kewaunee County, Wisconsin.

"Copyrighted 1895 by Rooney & Schleis.""E.P. Knoll & Co., Map Publishers, 8 North Sixth St., Phila., Pa.""Entered according to act of Congress in the year 1890 by Brice and Smith in the office of Librarian of Congress."LC Land ownership maps, 1426Includes business directory, population statistical table, list of county officers, views of public, and commercial properties.LC copy imperfect: Mounted on cloth backing, original sheets sectioned into 4 panels to enable folding. DLCLC copy hand colored and mounted on linen. DL

Resistance of canine lymphoma cells to adenoviral infection due to reduced cell surface RGD binding integrins

Recombinant adenovirus vectors (Ad) have been recognized as effective in vivo gene delivery vehicles and utilized as gene therapy agents for a number of cancers. The elucidation of viral entry mechanisms has allowed the development of recombinant vectors that exploit existing cell surface receptors to achieve entry into the cell. B lymphocytes are normally resistant to infection by adenovirus 5, likely due to the lack of the Coxsackie and Adenovirus receptor (CAR). Using reverse-transcriptase PCR and flow cytometry, the CD40 receptor has been shown to be expressed on many lymphoma cells. We exploited this finding to develop a gene therapy strategy for treatment of canine B-cell lymphoma. Ad5 was targeted to cells expressing CD40 via CD40 ligand (CD40L) and was effective in infecting CD40-expressing control cells; however, both primary canine lymphoma cells and cell lines demonstrated limited evidence of transduction. Following receptor binding, adenovirus entry into cells may require interaction with αvβ3/5 integrins; we demonstrate that canine lymphoma cells are deficient in these integrins. Reduced αvβ3 integrin expression may render these cells incapable of internalizing Ad vectors. Thus, any viral targeting approaches for treatment of canine lymphoma must also take into account the potential lack of internalization signals