Development of HIV-Resistant CAR T Cells by CRISPR/Cas-Mediated CAR Integration into the <i>CCR5</i> Locus

Adoptive immunotherapy using chimeric antigen receptor (CAR) T cells has been highly successful in treating B cell malignancies and holds great potential as a curative strategy for HIV infection. Recent advances in the use of anti-HIV broadly neutralizing antibodies (bNAbs) have provided vital information for optimal antigen targeting of CAR T cells. However, CD4+ CAR T cells are susceptible to HIV infection, limiting their therapeutic potential. In the current study, we engineered HIV-resistant CAR T cells using CRISPR/Cas9-mediated integration of a CAR cassette into the CCR5 locus. We used a single chain variable fragment (scFv) of the clinically potent bNAb 10-1074 as the antigen-targeting domain in our anti-HIV CAR T cells. Our anti-HIV CAR T cells showed specific lysis of HIV-infected cells in vitro. In a PBMC humanized mouse model of HIV infection, the anti-HIV CAR T cells expanded and transiently limited HIV infection. In conclusion, this study provides proof-of-concept for developing HIV-resistant CAR T cells using CRISPR/Cas9 targeted integration

Discovery of neutralizing SARS-CoV-2 antibodies enriched in a unique antigen specific B cell cluster.

Despite development of effective SARS-CoV-2 vaccines, a sub-group of vaccine non-responders depends on therapeutic antibodies or small-molecule drugs in cases of severe disease. However, perpetual viral evolution has required continuous efficacy monitoring as well as exploration of new therapeutic antibodies, to circumvent resistance mutations arising in the viral population. We performed SARS-CoV-2-specific B cell sorting and subsequent single-cell sequencing on material from 15 SARS-CoV-2 convalescent participants. Through screening of 455 monoclonal antibodies for SARS-CoV-2 variant binding and virus neutralization, we identified a cluster of activated B cells highly enriched for SARS-CoV-2 neutralizing antibodies. Epitope binning and Cryo-EM structure analysis identified the majority of neutralizing antibodies having epitopes overlapping with the ACE2 receptor binding motif (class 1 binders). Extensive functional antibody characterization identified two potent neutralizing antibodies, one retaining SARS-CoV-1 neutralizing capability, while both bind major common variants of concern and display prophylactic efficacy in vivo. The transcriptomic signature of activated B cells harboring broadly binding neutralizing antibodies with therapeutic potential identified here, may be a guide in future efforts of rapid therapeutic antibody discovery

In vitro analysis of lead mAbs binding.

(A) Ranking of purified mAbs from lowest IC50 pseudovirus neutralization value at the top (best neutralization) to highest IC50 at the bottom (poorest neutralization). Each antibody IC50 value obtained from triplicate point determinations of the dilution curve. Mesoscale binding values are shown for each mAb (supernatant) towards SARS-CoV-2 Spike, N-terminal domain (NTD) and receptor binding domain (RBD), as a heat-map. The binding determinations were performed once in duplicate. Colors indicate normalization from 0–100 within each column. (B) Mesoscale binding values for binding to the RBD of viral variants Alpha (N501Y, A570D), Beta (K417N, E484K, N501Y), Gamma (K417T, E484K, N501Y) and Delta (L452R), shown as fold change from SARS-CoV-2 RBD binding within each mAb individually. The binding determinations were performed once in duplicate. (C) Heat-map showing percentage ACE2 blocking for each mAb binding viral variant spike proteins (CoV-2, Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2) and Omicron BA.1(B.1.1.529). The ACE2 blocking analysis was performed in duplicate determinations of a dilution curve.</p

Characterization of mAb epitopes and cryo-EM structure of Omicron BA.1 spike trimer with Fab 29044.

(A) Epitope network plot colored by communities. Antibodies are represented as nodes and by number (Circles are mAbs tested in both orientations and squares are mAbs tested only in one direction). Pairwise blocking relationships are indicated by chords, and dashed lines are mAbs showing asymmetric blocking. A cut-off of 3.5 (S6B Fig) was used to define epitope communities. Community class III (Greens) and class II (Oranges) compete with community class I (magenta) but not with each other. (B) Epitope community plot highlighting mAbs retaining binding to Omicron B.1.1.529 and SARS-CoV-1 RBD colored in green. MAbs retaining binding to Omicron B.1.1.529 RBD is shown in light blue. (C) Surface representation of spike trimer overlayed with the cryo-EM density map (grey) is shown. Additional density observed for the Fab 29044 is circled and up- (red and green monomer) and down-RBD (blue monomer) are highlighted. (D) Zoomed in views showing model corresponding to variable region (Fv) of mAb 29044 (cartoon representation; VH, cyan; VL, pink) fitted into the overlayed Cryo-EM map (grey). (E) Close-up view of the complex depicting binding interface (yellow). (F) Labelled RBD residues (yellow) interfacing with 29044 Fv are shown. (G) Labelled ACE2 footprint (coral) on RBD in the background of 29044 Fv interface is shown.</p

Monoclonal antibody screening.

(A) Neutralization percentage of SARS-CoV-2 pseudovirus shown for each individual mAb supernatant analyzed at 20 μg/ml, shown on y-axis. MAbs are ordered along the x-axis from best (left) to poorest (right) neutralizers. n = 455. Screening was performed once in duplicate determinations. (B) Visualization of the expressed mAbs B cell cluster origin and distribution within all isolated B cells. Neutralizers shown in green ( neutralization, n = 9). Top neutralizers shown in red (<95% neutralization, n = 24). Non-neutralizers shown in blue (<80% neutralization). Background shown in grey (cells not expressed for screening).(C) Distribution of successful neutralizing mAbs, between clusters 6, 8 and remaining clusters (cluster 7 excluded). Hit rate cut-off for defining successful neutralizations was set at 80% pseudovirus neutralization. Hit rates were calculated within each cluster group. n = 455 (D) Predictive performance of Ag scores used for SARS-CoV-2 specific B cell sorting towards neutralization capability in cluster 8. n = 108. Red = SARS-CoV-2 D614G mutant trimer, Blue = SARS-CoV-2 RBD, Orange = SARS-CoV-1 RBD, Green = SARS-CoV-2 trimer. The p-value is based on a Kruskal-Wallis test of the receiver-operator characteristics curve.</p