Regulation of nodulation gene expression by NodD in Rhizobia

Microbial Biotechnolog

Subcellular localization of the Rhizobium leguminosarum nodl gene product

Microbial Biotechnolog

The NodD protein does not bind to the promoters of inducible nodulation genes in extracts of bacteroids of Rhizobium leguminosarum biovar viciae

Microbial Biotechnolog

Concurrent visualization of gusA and lacZ reporter gene expression

Here we describe conditions under which two reporter genes, encoding β-glucuronidase (gusA) and β-galactosidase (lacZ), can be used to simultaneously distinguish between the sites of expression of plant and bacterial genes, respectively, in symbiotic tissues. These conditions involve the fixation of cytoplasmic proteins with cold methanol before detecting gusA expression, and the addition of detergents to the incubation solution in order to inactivate endogenous plant β-galactosidases.Microbial Biotechnolog

Analysis of promoter activity of the early nodulin Enod40 in Lotus japonicus

Microbial Biotechnolog

Mutants in the nodFEL promoter of Rhizobium leguminosarum bv. viciae reveal a role of individual nucleotides in transcriptional activation and protein binding

Microbial Biotechnolog

Suppression of nodulation gene expression in bacteroids of Rhizobium leguminosarum biovar viciae

Microbial Biotechnolog

Subcellular localization of the nodD gene product in Rhizobium leguminosarum

Microbial Biotechnolog

Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize rhizobia interacting with plants

Microbial Biotechnolog

Auxin transport inhibition precedes root nodule formation in white clover roots and is regulated by flavonoids and derivatives of chitin oligosaccharides

Plant science