42 research outputs found
Expression of multidrug resistance genes MVP, MDR1, and MRP1 determined sequentially before, during, and after hyperthermic isolated limb perfusion of soft tissue sarcoma and melanoma patients
Purpose: Isolated, hyperthermic limb perfusion (ILP) with recombinant human tumor necrosis factor alpha and melphalan is a highly effective treatment for advanced soft tissue sarcoma (STS) and locoregional metastatic malignant melanoma. Multidrug resistance (MDR)-associated genes are known to be inducible by heat and drugs; expression levels of the major vault protein (MVP), MDR1, and MDR-associated protein 1 (MRP1) were determined sequentially before, during, and after ILP of patients. Patients and Methods: Twenty-one STS or malignant melanoma patients were treated by ILP. Tumor tissue temperatures were recorded continuously and ranged from 33.4°C initially to peak values of 40.4°C during ILP. Serial true-cut biopsy specimens from tumor tissues were routinely microdissected. Expression analyses for MDR genes were performed by real-time reverse transcriptase polymerase chain reaction and immunohistochemistry. Results: In 83% of the patients, MVP expression was induced during hyperthermic ILP. MVP-mRNA inductions often paralleled the increase in temperature during ILP. Increased MVP protein expressions either were observed simultaneously with the MVP-mRNA induction or were delayed until after the induction at the transcriptional level. Inductions of MDR1 and MRP1 were observed in only 13% and 27% of the specimens analyzed. Temperatures and drugs applied preferentially led to an induction of MVP and were not sufficient to induce MDR1 and MRP1 in the majority of tumors. Conclusion: This study is the first to analyze the expression of MDR-associated genes sequentially during ILP of patients and demonstrates that treatment might lead to increased levels of MVP, whereas enhanced levels of MDR1 and MRP1 remain rare events
Breathing, transcutaneous blood gases, and CO2 response in SIDS siblings and control infants during sleep
The caudal pressor area - A new region of the ventrolateral medulla involved in cardiovascular regulation
ESCOLA PAULISTA MED,DEPT PHYSIOL,SAO PAULO,BRAZILESCOLA PAULISTA MED,DEPT PHYSIOL,SAO PAULO,BRAZILWeb of Scienc
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Design of 24. 8-kW, 3. 8 K cryogenic system for ISABELLE
ISABELLE will consist of two proton accelerator/storage rings in a common tunnel. There will be 1084 superconducting magnets installed in the 3.8 km circumference tunnel. The protons will be accelerated to an energy of 400 GeV in each ring and stored for 24 hours. The two beams are counter-rotating and intersect at six places around the ring. The particles which emerge from proton interactions at these intersecting points are studied using various types of detectors
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Operational tests of the BNL 24. 8 kW, 3. 8 K helium refrigerator
The BNL 24.8 kW refrigeration system is completely installed and major portions of the acceptance tests have been completed. So far, the equipment tested has performed at or above design levels. The room temperature helium compressor station has been completely tested and accepted. The two-stage oil injected screw compressor system exhibited an isothermal efficiency of 57% while delivering a helium flow in excess of 4400 g/s. Data on the performance of the make-up gas cryogenic purifier is given. The refrigerator turbomachinery, 13 expanders and three cold compressors, has been tested at room temperature for mechanical integrity and control stability. The first cooldown to operating temperature will be attempted in late August, 1985. 2 refs., 5 figs