Numerical and Experimental Analysis of the Pressure Signature for different High-Speed Trains

This paper describes a procedure for the validation of numerical codes able to reproduce the pressures in tunnel due to the passage of trains. In the first step, the parameters of the numerical code are set by matching the train-tunnel pressure signature measured during a single-passage of different types of train within the tunnel and in the second step, without changing the parameters, the crossing of two trains is simulated. Within the paper, the methodology is applied to the numerical mono-dimensional code DB-Tunnel while the experimental data are those collected during an experimental research programme carried out in the tunnel La Fornace, on the Italian high-speed railway from Roma to Firenze. The accuracy of the numerical code estimation is evaluated in terms of the maximum pressure generated in the tunnel by the train passing/crossing because this is the key parameter, according to the TSI standard for railway infrastructures

Simplified estimation of the train resistance parameters: full scale experimental tests and analysis

A CEN standard (EN 14067-4, 2005) describes the methodologies for the assessment of the running resistance of railway vehicles starting from full-scale test measurements. According to this standard, the speed dependent terms of the equation of Davis [1] have to be determined by means of coasting tests. In this paper, a new method to estimate the running resistance coefficients from a full-scale coasting test is proposed and compared with the two methods proposed in the CEN standard (the regression method and the speed history identification method). The main advantage of this new method is that it does not require the railway line characteristics to be known and it will be shown that the new method is able to evaluate the coefficients with an accuracy equivalent to that of the other methods considered

Codes and standards on computational wind engineering for structural design: State of art and recent trends

This paper first provides a wide overview about the design codes and standards covering the use of Computational Wind Engineering / Computational Fluid Dynamics (CWE/CFD) for wind-sensitive structures and built environment. Second, the paper sets out the basic assumptions and underlying concepts of the new Annex T "Simulations by Computational Fluid Dynamics (CFD/CWE)" of the revised version "Guide for the assessment of wind actions and effects on structures" issued by the Advisory Committee on Technical Recommendations for Constructions of the Italian National Research Council in February 2019 and drafted by the members of the Special Interest Group on Computational Wind Engineering of the Italian Association for Wind Engineering (ANIV-CWE). The same group is currently advising UNI CT021/SC1 in supporting the drafting of the new Annex K - "Derivation of design parameters from wind tunnel tests and numerical simulations" of the revised Eurocode 1: Actions on structures - Part 1-4: General actions - Wind actions. Finally, the paper outlines the subjects most open to development at the technical and applicative level

Procalcitonin-guided antibiotic use versus a standard approach for acute respiratory tract infections in primary care: study protocol for a randomised controlled trial and baseline characteristics of participating general practitioners [ISRCTN73182671]

BACKGROUND: Acute respiratory tract infections (ARTI) are among the most frequent reasons for consultations in primary care. Although predominantly viral in origin, ARTI often lead to the prescription of antibiotics for ambulatory patients, mainly because it is difficult to distinguish between viral and bacterial infections. Unnecessary antibiotic use, however, is associated with increased drug expenditure, side effects and antibiotic resistance. A novel approach is to guide antibiotic therapy by procalcitonin (ProCT), since serum levels of ProCT are elevated in bacterial infections but remain lower in viral infections and inflammatory diseases. The aim of this trial is to compare a ProCT-guided antibiotic therapy with a standard approach based on evidence-based guidelines for patients with ARTI in primary care. METHODS/DESIGN: This is a randomised controlled trial in primary care with an open intervention. Adult patients judged by their general practitioner (GP) to need antibiotics for ARTI are randomised in equal numbers either to standard antibiotic therapy or to ProCT-guided antibiotic therapy. Patients are followed-up after 1 week by their GP and after 2 and 4 weeks by phone interviews carried out by medical students blinded to the goal of the trial. Exclusion criteria for patients are antibiotic use in the previous 28 days, psychiatric disorders or inability to give written informed consent, not being fluent in German, severe immunosuppression, intravenous drug use, cystic fibrosis, active tuberculosis, or need for immediate hospitalisation. The primary endpoint is days with restrictions from ARTI within 14 days after randomisation. Secondary outcomes are antibiotic use in terms of antibiotic prescription rate and duration of antibiotic treatment in days, days off work and days with side-effects from medication within 14 days, and relapse rate from the infection within 28 days after randomisation. DISCUSSION: We aim to include 600 patients from 50 general practices in the Northwest of Switzerland. Data from the registry of the Swiss Medical Association suggests that our recruited GPs are representative of all eligible GPs with respect to age, proportion of female physicians, specialisation, years of postgraduate training and years in private practice

The type 2C phosphatase Wip1: An oncogenic regulator of tumor suppressor and DNA damage response pathways

The Wild-type p53-induced phosphatase 1, Wip1 (or PPM1D), is unusual in that it is a serine/threonine phosphatase with oncogenic activity. A member of the type 2C phosphatases (PP2Cδ), Wip1 has been shown to be amplified and overexpressed in multiple human cancer types, including breast and ovarian carcinomas. In rodent primary fibroblast transformation assays, Wip1 cooperates with known oncogenes to induce transformed foci. The recent identification of target proteins that are dephosphorylated by Wip1 has provided mechanistic insights into its oncogenic functions. Wip1 acts as a homeostatic regulator of the DNA damage response by dephosphorylating proteins that are substrates of both ATM and ATR, important DNA damage sensor kinases. Wip1 also suppresses the activity of multiple tumor suppressors, including p53, ATM, p16INK4a and ARF. We present evidence that the suppression of p53, p38 MAP kinase, and ATM/ATR signaling pathways by Wip1 are important components of its oncogenicity when it is amplified and overexpressed in human cancers