Towards Flipped Learning in Upper Secondary Mathematics Education

Challenges for students in the 21st century, such as acquiring technology, problem-solving, and cooperation skills, also necessitate changes in mathematics education to be able to respond to changing educational needs. One way to respond to these challenges is by utilizing recent educational innovations in schools, for instance, among others are flipped learning (FL) approaches. In this paper, we outline our explorative educational experiment that investigates vital elements of mathematics learning in FL approaches in upper secondary education. We describe the methodologies and findings of our qualitative study based on design-based research to discover key elements of FL approaches in upper secondary education. Analyzing the oral and written data collected over ten months using grounded theory approaches suggested categories (a) confidence when learning; (b) learning by working, and, and (c) flexibility when learning could be essential to understand FL approaches practices in mathematics classrooms. These categories indicate that when using FL approaches in mathematics learning, it could be essential for students to acquire knowledge in a confident and adaptable environment actively

Informing Management of Atlantic Bluefin Tuna Using Telemetry Data

Sustainable management of exploited marine fish and wildlife populations requires knowledge about their productivity. Survival from natural causes of mortality is a key component of population productivity, but is notoriously difficult to estimate. We evaluate the potential for acoustic telemetry as a long-term monitoring tool to estimate rates of natural mortality. We present a Bayesian multistate mark-recapture model for telemetry data collected over a decade from 188 Atlantic bluefin tuna (Thunnus thynnus) and apply it to estimate the rate of natural mortality using only acoustic tag detections for all animals, or using acoustic tag detections for 96 single-tagged tuna plus acoustic tag detections combined with estimated positions from pop-up satellite archival tags for 92 double-tagged animals. We support the model for bluefin tuna with a simulation study to quantify bias in estimates of population dynamics parameters and investigate the effect of auxiliary information from satellite tagging on mortality rate estimates for different acoustic tag detection probability scenarios.We obtained posterior estimates of the instantaneous annual natural mortality (survival) rate across a decade of tagging for Atlantic bluefin tuna of 0.17 yr(-1) (0.84 yr(-1)) both using only acoustic tagging data, and using a combination of acoustic and satellite tagging data. Use of a prior implying a higher rate of fishing mortality yielded an instantaneous annual natural mortality (survival) estimate of 0.10 yr(-1) (0.90 yr(-1)), with combined acoustic and satellite tag data. Results from the simulation study indicate that the use of satellite tags can improve the precision and accuracy of estimates of detection probabilities, area-specific movement probabilities and mortality rates, where the extent of the improvement depends on true underlying acoustic tag detection probabilities. Our work demonstrates that long-term acoustic tagging data sets have strong potential for monitoring of highly migratory marine fish and wildlife populations, providing information on a number of key parameters, including survival and movement rates. However, improved information on tag reporting rates or fishing mortality is needed to better separate natural and fisheries mortality for Atlantic bluefin tuna

Temperature Effects on Metabolic Rate of Juvenile Pacific Bluefin Tuna \u3ci\u3eThunnus Orientalis\u3c/i\u3e

Pacific bluefin tuna inhabit a wide range of thermal environments across the Pacific ocean. To examine how metabolism varies across this thermal range, we studied the effect of ambient water temperature on metabolic rate of juvenile Pacific bluefin tuna, Thunnus thynnus, swimming in a swim tunnel. Rate of oxygen consumption (MO2) was measured at ambient temperatures of 8–25°C and swimming speeds of 0.75–1.75 body lengths (BL) s–1. Pacific bluefin swimming at 1 BL s–1 per second exhibited a U-shaped curve of metabolic rate vs ambient temperature, with a thermal minimum zone between 15°C to 20°C. Minimum MO2 of 175±29 mg kg–1 h–1–1 was recorded at 15°C, while both cold and warm temperatures resulted in increased metabolic rates of 331±62 mg kg–1 h–1–1 at 8°C and 256±19 mg kg–1 h–1–1 at 25°C. Tailbeat frequencies were negatively correlated with ambient temperature. Additional experiments indicated that the increase in MO2 at low temperature occurred only at low swimming speeds. Ambient water temperature data from electronic tags implanted in wild fish indicate that Pacific bluefin of similar size to the experimental fish used in the swim tunnel spend most of their time in ambient temperatures in the metabolic thermal minimum zone

Atlantic Bluefin Tuna (Thunnus thynnus) Diet in the Gulf of St. Lawrence and on the Eastern Scotian Shelf

The stomach contents of 68 Atlantic bluefin tuna (Thunnus thynnus) landed in Port Hood and Canso, Nova Scotia, in 2010, were analyzed to characterize the diet of bluefin tuna at the two locations. Of the sampled fish, 54 stomachs had contents. Pelagic schooling fish such as herring (Clupea harengus) and mackerel (Scomber scombrus) dominated the diets in both regions. However, a number of rare species, including demersal species, were also observed. Despite the difference in location and the significantly larger size of the Atlantic bluefin tuna landed in Port Hood, the diets of the Atlantic bluefin tuna landed at both sites were similar

Pop-up archival tags reveal environmental influences on the vertical movements of silvertip sharks (Carcharhinus albimarginatus)

Vertical space use informs the ecology and management of marine species, but studies of reef-associated sharks often focus on horizontal movements. We analysed the vertical movements of silvertip sharks (Carcharhinus albimarginatus) using pop-up archival tags deployed on seven individuals in the Chagos Archipelago, central Indian Ocean. The sharks changed depth predictably with water column thermal structure, moving deeper with seasonal increases in mixed layer depth while occupying a narrow ambient water temperature range around ~27°C. At shorter timescales, higher resolution data from five tags showed that silvertip shark depth varied cyclically with surface light levels, increasing during daylight and on nights around full moon. This matches the diel vertical migrations of many fish species, suggesting the sharks’ light-driven depth changes might relate to foraging. While most vertical movements (>98%) were within the mixed layer, deeper dives to 200-800 m occurred approximately every three days. High-resolution data from one recovered tag showed the shark ascending deep (>200 m) dives in two sharply defined phases, initially fast then slow. Analysis of dive profiles against dissolved oxygen (DO) data suggested that the shark may have ascended rapidly to escape low DO levels at depth, then reduced its ascent rate 50-80% once DO levels increased. While a small sample, the electronic tags deployed in this study revealed the silvertip sharks’ predictable use of mixed layer waters, narrow thermal range and apparent intolerance of hypoxic conditions. These characteristics may exacerbate the species’ vulnerability as oceanic warming and shoaling oxygen minimum zones modify vertical habitat availability

Detecting Mediterranean White Sharks with Environmental DNA

The white shark (Carcharodon carcharias) is a globally distributed, ecologically important top predator whose biology and population dynamics are challenging to study. Basic biological parameters remain virtually unknown in the Mediterranean Sea due to its historically low population density, dwindling population size, and lack of substantial sightings. White sharks are considered Critically Endangered in the Mediterranean Sea, and recent analyses suggest that the population has declined by 52% to 96% from historical levels in different Mediterranean sectors (Moro et al., 2020). Thus, white shark sightings dating back to 1860 are being used to estimate population trajectories throughout the entire region. Though the population size is unknown, remaining individuals are thought to be primarily restricted to a handful of hotspots deemed important for their reproduction and foraging. One of these hypothesized hotspots is the Sicilian Channel, which accounts for 19% of total historical sightings

Assignment of tracks from tagged Atlantic bluefin tuna Thunnus thynnus to potential stocks using behavioural differences and habitat partitioning

IntroductionAtlantic bluefin tuna (ABT) are large, migratory pelagic predators of high economic importance. ABT are currently managed as two independent stocks assigned to discrete spawning areas (Gulf of Mexico, and Mediterranean); however, stock overlap outside spawning areas makes accurate assignment of catch to stock-of-origin difficult.MethodsWithin this two-stock paradigm, we characterised stock-specific spatial distributions and behaviours by comparing habitat usage and vertical movement behaviours of 118 electronically tagged adult ABT spatially assigned to the GOM and Med spawning grounds. These spatial and behavioural differences were used in tests to probabilistically assign unknown individuals (which did not visit the GOM/Med spawning areas) to a stock.ResultsThis new methodological approach using existing tag data, enables increased assignment of a track to a potential stock, to be achieved before genetic assignments. We identified certain markedly different movement patterns, range extents, depth use preferences (and associated area usage), migration directness and speeds, corresponding distance from shore, and mesopelagic-layer visitation. The probabilistic assignment approach had 97% in-bag testing accuracy, then assigned 190 individuals to a stock, doubling the number of assigned stock-of-origin tracks in our dataset, and potentially revealing novel movement and behaviour patterns among pre-spawning-age ABT.DiscussionThis approach can be easily adapted to other study species, more stocks, and different testing variables, hopefully serving as a useful addition to the fisheries management toolkit

Estimating Space Use of Mobile Fishes in a Large Marine Protected Area With Methodological Considerations in Acoustic Array Design

Marine protected areas (MPAs) have become an increasingly important tool to protect and conserve marine resources. However, there remains much debate about how effective MPAs are, especially in terms of their ability to protect mobile marine species such as teleost and chondrichthyan fishes. We used satellite and acoustic tags to assess the ability of a large oceanic MPA, the British Indian Ocean Territory MPA (BIOT MPA), to protect seven species of pelagic and reef-associated teleost and chondrichthyan fishes. We satellite-tagged 26 animals from six species (Blue Marlin, Reef Mantas, Sailfish, Silky Sharks, Silvertip Sharks, and Yellowfin Tuna), producing 2,735 days of movement data. We also acoustically tagged 121 sharks from two species (Grey Reef and Silvertip Sharks), which were monitored for up to 40 months across a large acoustic receiver array spanning the MPA. We found that the activity spaces of all satellite-tagged animals, including pelagic species, were much smaller than the area of the BIOT MPA, even taking into account errors associated with position estimates. Estimates of space use of acoustically tagged sharks, based on dynamic Brownian Bridge Movement Models (dBBMM), were also much smaller than the size of the MPA. However, we found important limitations when using dBBMM and demonstrate its sensitivity to both study duration and array design. We found that Grey Reef Sharks should be monitored for at least 1 year and Silvertip Sharks for 2 years before their activity space can be effectively estimated. We also demonstrate the potentially important role that intraspecific variability in spatial ecology may play in influencing the ability of MPAs to effectively protect populations of mobile species. Overall, our results suggest that, with effective enforcement, MPAs on the scale of the BIOT MPA potentially offer protection to a variety of pelagic and reef species with a range of spatial ecologies. We suggest that animals need to be tagged across seasons, years, and ontogenetic stages, in order to fully characterize their spatial ecology, which is fundamental to developing and implementing effective MPAs to conserve the full life history of target species

The neuroprotective and neurorescue effects of carbamylated erythropoietin Fc fusion protein (CEPO-Fc) in a rat model of Parkinson's disease

Parkinson's disease is characterized by progressive degeneration of dopaminergic neurons. Thus the development of therapeutic neuroprotection and neurorescue strategies to mitigate disease progression is important. In this study we evaluated the neuroprotective/rescue effects of erythropoietin Fc fusion protein (EPO-Fc) and carbamylated erythropoietin Fe fusion protein (CEPO-Fc) in a rat model of Parkinson's disease. Adult female Sprague-Dawley rats received intraperitoneal injection of EPO-Fc, CEPO-Fc or PBS. Behavioral evaluations consisted of rota-rod, cylinder and amphetamine-induced rotation tests. In the neuroprotection experiment, the CEPO-Fc group demonstrated significant improvement compared with the EPO-Fc group on the amphetamine-induced rotation test throughout the four-week follow-up period. Histologically, significantly more tyrosine hydroxylase (TH)-positive neurons were recognized in the substantia nigra (SN) pars compacta in the CEPO-Fc group than in the PBS and EPO-Fc groups. In the neurorescue experiment, rats receiving CEPO-Fc showed significantly better behavioural scores than those receiving PBS. The histological data concerning striatum also showed that the CEPO-Fc group had significantly better preservation of TH-positive fibers compared to the PBS and EPO-Fc groups. Importantly, there were no increases in hematocrit or hemoglobin levels in the CEPO-Fc group in either the neuroprotection or the neurorescue experiments. In conclusion, the newly developed CEPO-Fc might confer neuroprotective and neurorescue benefits in a rat model of Parkinson's disease without the side effects associated with polycythemia. CEPO-Fc might be a therapeutic tool for patients with Parkinson's disease