23 research outputs found
Application of self-assembly techniques in the design of biocompatible protein microarray surfaces
This review focuses on the application of novel technologies for generating biocompatible surfaces for high-throughput screening (HTS) of proteins. Various methods of coupling and spotting proteins on self-assembled monolayer (SAM) surfaces will be described along with the protein chip challenges pertaining to spot homogeneity, morphology, biocompatibility and reproducibility
Application of self-assembly techniques in the design of biocompatible protein microarray surfaces
This review focuses on the application of novel technologies for generating biocompatible surfaces for high-throughput screening (HTS) of proteins. Various methods of coupling and spotting proteins on self-assembled monolayer (SAM) surfaces will be described along with the protein chip challenges pertaining to spot homogeneity, morphology, biocompatibility and reproducibility
Time-resolved enzymatic determination of glucose using a fluorescent europium probe for hydrogen peroxide
An enzymatic assay for glucose based on the use of the fluorescent probe for hydrogen peroxide, europium(III) tetracycline (EuTc), is described. The weakly fluorescent EuTc and enzymatically generated H2O2 form a strongly fluorescent complex (EuTcâH2O2) whose fluorescence decay profile is significantly different. Since the decay time of EuTcâH2O2 is in the microseconds time domain, fluorescence can be detected in the time-resolved mode, thus enabling substantial reduction of background fluorescence. The scheme represents the first H2O2-based time-resolved fluorescence assay for glucose not requiring the presence of a peroxidase. The time-resolved assay (with a delay time of 60 mgrs and using endpoint detection) enables glucose to be determined at levels as low as 2.2 mgrmol Lâ1, with a dynamic range of 2.2â100 mgrmol Lâ1. The method also was adapted to a kinetic assay in order to cover higher glucose levels (mmol Lâ1 range). The latter was validated by analyzing spiked serum samples and gave a good linear relationship for glucose levels from 2.5 to 55.5 mmol Lâ1. Noteworthy features of the assay include easy accessibility of the probe, large Stokesrsquo shift, a line-like fluorescence peaking at 616 nm, stability towards oxygen, a working pH of approximately 7, and its suitability for both kinetic and endpoint determination