9 research outputs found
Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
- Author
- A Bebenek
- A Johnson
- A Skerra
- Audrey J. Klingele
- B Liss
- B Witzenbichler
- C Fehlmann
- CA Suttle
- D Canceill
- D Liu
- DA Mead
- Darby R. Sugar
- David A. Mead
- DK Braithwaite
- DP Chandler
- E Sellmann
- EK Halvas
- EQ Konnick
- EQ Konnick
- ES Svarovskaia
- FB Perler
- FC Lawyer
- GF Gerard
- H Li
- HE Huber
- HH Hogrefe
- I Desombere
- J Cline
- J Dreier
- J Sambrook
- J Van Ness
- JD Roberts
- JL Brincat
- JL Mbisa
- John E. Tavis
- JP Levesque-Sergerie
- K Sauter
- KJ Livak
- KM Chumakov
- KP O'Connell
- Krishne Gowda
- KS Lundberg
- L Blanco
- LJ Coyle DE
- LN Sellner
- LV Lotti
- M Ricchetti
- Michael J. Moser
- ML Kotewicz
- N Andraos
- N Schönbrunner
- O Suslov
- P Bolufer
- RA Bambara
- Robert A. DiFrancesco
- S Creighton
- S Tabor
- SF Altschul
- SM Lima
- SP Goff
- Stacy Stocki
- T Ando
- T Notomi
- T Schoenfeld
- T Schoenfeld
- Thomas W. Schoenfeld
- TW Myers
- V Derbyshire
- V Lyamichev
- W Fiers
- Y Kurihara
- Publication venue
- Public Library of Science
- Publication date
- 04/06/2012
- Field of study
Viral metagenomic libraries are a promising but previously untapped source of new reagent enzymes. Deep sequencing and functional screening of viral metagenomic DNA from a near-boiling thermal pool identified clones expressing thermostable DNA polymerase (Pol) activity. Among these, 3173 Pol demonstrated both high thermostability and innate reverse transcriptase (RT) activity. We describe the biochemistry of 3173 Pol and report its use in single-enzyme reverse transcription PCR (RT-PCR). Wild-type 3173 Pol contains a proofreading 3′-5′ exonuclease domain that confers high fidelity in PCR. An easier-to-use exonuclease-deficient derivative was incorporated into a PyroScript RT-PCR master mix and compared to one-enzyme (Tth) and two-enzyme (MMLV RT/Taq) RT-PCR systems for quantitative detection of MS2 RNA, influenza A RNA, and mRNA targets. Specificity and sensitivity of 3173 Pol-based RT-PCR were higher than Tth Pol and comparable to three common two-enzyme systems. The performance and simplified set-up make this enzyme a potential alternative for research and molecular diagnostics
Body condition and energy density of juvenile streaked prochilod Prochilodus lineatus (Valenciennes, 1837) in a Neotropical floodplain
- Author
- Abujanra F
- Adams S. M
- Adams S. M
- Agostinho A. A
- Agostinho A. A
- Agostinho A. A
- Agostinho A. A
- Agostinho A. A
- Agostinho A. A
- Algarte V. M
- Ana LĂşcia Veronezzi
- Bajer P. G
- Barbieri G
- Bayo V
- Bevelhimer M. S
- Block W. M
- Bowen S. H
- Bozza A. N
- Breukelaar A. W
- Camargo V. M
- Carapurnala L
- Chimney M. J
- Cui Y
- Daiany de Fátima Corbetta
- Dourado E. C. S
- Enrich-Prast A
- EspĂnola L. A
- EspĂnola L. A
- Evanilde Benedito
- Evans R. D
- Froese R
- Froese R
- Garcia D. A
- GarcĂa-Berthou E
- Gimenes M. F
- Godoy M. P
- Gomes L. C
- Graça W. J
- Hahn N. S
- Hahn N. S
- Herbinger C. M
- Junk W. J
- Karling L. C
- Le Cren E. D
- Leandrini J. A
- Lee A. A
- Lindeman R. L
- Luz-Agostinho K. D. G
- Monteiro V
- Neiff J. J
- Oliveira A. G
- Pagioro T. A
- Petrere JĂşnior M
- Resende E. K
- Roberto M. C
- Rodrigues L. C
- Sacramento P. A
- Saldaña J
- Santos A. F. G. N
- Santos M. H. dos
- Schork G
- Schönbrunner I. M
- Silva E. M
- Sivasundar A
- Sokal R. R
- Suzuki H. I
- Taniguchi G. M
- Thomaz S. M
- Thomaz S. M
- Vanzolini P. E
- Vazzoler A. E. A. M
- Yahdjian L
- Zar J. H
- Publication venue
- 'FapUNIFESP (SciELO)'
- Publication date
- 01/01/2016
- Field of study
Mechanism of Helix Induction by Trifluoroethanol: A Framework for Extrapolating the Helix-Forming Properties of Peptides from Trifluoroethanol/Water Mixtures Back to Water
- Author
- Abbreviations TFE
- Alexandrescu A. T.
- Applequist J.
- Bates R.
- Behrends H. W.
- Ben-Naim A.
- Bixon M.
- Blanco F. J.
- Brandts J. R.
- Buch M.
- Buck M.
- Cammers-Goodwin A.
- Chakrabartty A.
- Chakrabartty A.
- Chakrabartty A.
- Chen Y.-H.
- Conio G.
- Doig A. J.
- Dyson H. J.
- Fan P.
- Filippi B.
- Goodman M.
- Hamada D.
- Hermans J. Jr.
- Jasanoff A.
- Johnson M. L.
- Lifson S.
- Lotan N.
- Muñoz V.
- Nelson J. W.
- RamĂrez-Alvarado M.
- Rohl C. A.
- Rohl C. A.
- Rohl C. A.
- Scholtz J. M.
- Scholtz J. M.
- Scholtz J. M.
- Schönbrunner N.
- Segawa S-I.
- Shan S.-O.
- Shin H.-C.
- Shin H.-C.
- Smith J. S.
- Storrs R. W.
- Sönnichsen F. D.
- Waltho J.
- Wang J.
- Yang A. S.
- Zimm B. H.
- Zimm B. H.
- Publication venue
- 'American Chemical Society (ACS)'
- Publication date
- Field of study
Actinobacteria Cyclophilins: Phylogenetic Relationships and Description of New Class- and Order-Specific Paralogues
- Author
- A Hochman
- A Manteca
- A Manteca
- A Pahl
- A Pahl
- A Paradkar
- Ana I. Pelaez
- Angel Manteca
- B Janowski
- B Schmidt
- BD Kerger
- C Mendez
- C Pissavin
- CJ Leonard
- DA Hopwood
- DL Swofford
- ER Schönbrunner
- EV Koonin
- EV Koonin
- F Abascal
- G Stoller
- GR Nicieza
- H Bang
- H Ikeda
- HF Clark
- J Devereux
- J Liu
- J Liu
- J Sambrook
- Jesus Sanchez
- JP Huelsenbeck
- JR Cole
- K Nagashima
- K Petrickova
- K Ueda
- KF Chater
- KF Chater
- KF Chater
- L Aravind
- L Shi
- LA Compton
- LH Zhang
- LM Henriksson
- M Herrler
- M Herrler
- M Konno
- M Metsä-Ketelä
- M Norregaard-Madsen
- MB Yarmolinsky
- MC Martin
- MM McNeil
- MO Dayhoff
- MR Lindsay
- MTG Ivery
- N Guex
- N Guex
- OH Lowry
- P Boer
- P Wang
- P Wiener
- Rafael Zardoya
- RC Edgar
- RG Kok
- RH Baltz
- RT Clubb
- S Altschul
- S Egan
- S Garcia-Vallve
- S Guindon
- S Schneuwly
- SD Bentley
- SF Göthel
- SF Göthel
- SK Anderson
- T Hayano
- T Kawase
- T Maruyama
- T Schwede
- TV Achenbach
- UK Laemmli
- V Mikol
- WP Maddison
- Y Nishio
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Effects of drying on phosphorus uptake in re-flooded lake sediments
- Author
- A Fabre
- A Kleeberg
- A Kleeberg
- A Kleeberg
- A Kleeberg
- A Mitchell
- A Twinch
- AD Steinman
- AJ McComb
- B Nguyen
- B Turner
- B Turner
- C Dahm
- C Laskov
- C Watts
- C Watts
- Christiane Herzog
- CJ Groot de
- CJ Groot de
- D Baldwin
- D Baldwin
- D Baldwin
- D Zak
- Daniela Dieter
- E Driescher
- EE Roden
- EO Young
- H Birch
- H Coops
- I Vicente de
- IM Schönbrunner
- J Kerr
- J Murphy
- J Wichelen Van
- JD Gilbert
- JS Wilson
- JT Smit
- K Reddy
- K Tockner
- KE Havens
- KM Wantzen
- KM Wantzen
- L Kamp-Nielsen
- L Lijklema
- LE Fox
- M Holmer
- M Hupfer
- Michael Hupfer
- N Caraco
- NJ Barrow
- NJ Barrow
- P Grierson
- P Lake
- P Nair
- PJA Withers
- R Corstanje
- R Ihaka
- R Psenner
- RC Aller
- S Brand-Klibanski
- S Brothers
- S Qiu
- S Qiu
- S Qiu
- WJ Xiao
- X Tang
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Solvent Perturbation of Protein Structures - A Review Study with Lectins
- Author
- Abedini A.
- Anderson V.L.
- Anderson V.L.
- Anfinsen C.B.
- Anisur R. Molla
- Bai J.H.
- Baldwin R.L.
- Banerjee R.
- Banerjee V.
- Bhattacharjya S.
- Bhattacharjya S.
- Brockwell D.J.
- Buck M.
- Buck M.
- Campos J.K.L.
- Chandra N.R.
- Chen E.
- Chikalovets I.V.
- Couthon F.
- Dammers C.
- Deacon A.
- Decastel M.
- Dev S.
- Di Carlo M.G.
- Dobson C.M.
- Dobson C.M.
- Edelman G.M.
- Einspahr H.
- Fatima S.
- Fezoui Y.
- Fink A.L.
- Fujita-Yamaguchi Y.
- Gast K.
- Ghosh M.
- Greene R.F.
- Greff D.
- Griebenow K.
- Hamada D.
- Hardman K.D.
- Hendrickson O.D.
- Herskovits T.T.
- Holm N.K.
- Houston L.L.
- Jackson M.
- Jackson M.
- Jordens S.
- Kelly S.M.
- Kemmink J.
- Khan M.V.
- Kim P.S.
- Konno T.
- Lehrman S.R.
- Lequin O.
- Levinthal C.
- Liao D.I.
- Lim V.I.
- Lis H.
- Loris R.
- Loris R.
- Mandal P.
- Mandal P.
- Marion D.
- Matsumura Y.
- Molla A.R.
- Molla A.R.
- Munishkina L.A.
- Naqvi Z.
- Naseem F.
- Naseem F.
- Naseem F.
- Naseem F.
- Naseem F.
- Nelson J.W.
- Olsen L.R.
- Pallarès I.
- Perham M.
- Pritha Mandal
- Rezaei-Ghaleh N.
- Santangelo M.G.
- Schönbrunner N.
- Sen D.
- Sen P.
- Shammas S.L.
- Sharon N.
- Sharon N.
- Shiraki K.
- Siligardi G.
- Singh K.
- Spande T.F.
- Sreerama N.
- Srisailam S.
- Sticht H.
- Stillmark H.
- Sundd M.
- Svensson C.
- Sönnichsen F.D.
- Tanford C.
- Varshney A.
- Vetri V.
- Vymětal J.
- Wetlaufer D.B.
- Wilkinson K.D.
- Xiong K.
- Xu Q.
- Yamasaki N.
- Publication venue
- 'Bentham Science Publishers Ltd.'
- Publication date
- Field of study
Carbon and nutrient cycling in kettle hole sediments depending on hydrological dynamics: a review
- Author
- A Barthès
- A Bollmann
- A Foulquier
- A Kleeberg
- A Thompson
- AD Steinman
- AE Miller
- AJ Burgin
- AJ Tessier
- AL Peralta
- AM Hartley
- AM Mitchell
- B Oertli
- B Palik
- B Waldon
- BM Spears
- C Callieri
- C Freeman
- C Gudasz
- C Verpoorter
- C-J Groot De
- CE Oldham
- CJ Groot De
- CJ Schubert
- CL Roehm
- CM Michmerhuizen
- D Bastviken
- D Billi
- D Dieter
- D Zak
- DJ Dowrick
- DS Baldwin
- DS Baldwin
- DW Schindler
- E Pohlon
- ER Hunting
- F Althoff
- F Keppler
- F Navarro-GarcĂa
- F Rezanezhad
- F Sgouridis
- F Wang
- Florian Reverey
- G Borrel
- G Eller
- G Hulthe
- G Kamp Van der
- G Lischeid
- G Lischeid
- G Pinay
- Gunnar Lischeid
- Hans-Peter Grossart
- HD KlĂĽber
- HH Gerke
- HP Grossart
- I Schmidt
- I Vicente De
- IC Torres
- IM Schönbrunner
- J Biggs
- J Kopáček
- J Schimel
- JA Andrews
- JA Downing
- JA Downing
- JA Downing
- JC Yuste
- JM Stark
- JP Megonigal
- JS Read
- JT Scott
- K Attermeyer
- K Denef
- K Felsmann
- K-H Knorr
- Katrin Premke
- KF Ettwig
- KH Knorr
- KH Wyatt
- KR Reddy
- KR Reddy
- KR Reddy
- L Lijklema
- L Palomo
- L Serrano
- LM Mayer
- LM Mosley
- LM Mosley
- M Aeschbacher
- M Bouvy
- M Fernandez-Aláez
- M Gette-Bouvarot
- M Holmer
- M Hupfer
- M Keiluweit
- M Pätzig
- M Sahuquillo
- M Santos Afonso Dos
- M Scheffer
- M Strous
- M Søndergaard
- M Søndergaard
- M Søndergaard
- M Thullner
- MA Holgerson
- MC Eimers
- ME McClain
- MF Haroon
- MG Grosswald
- MI Arce
- ML Kakumanu
- MM Mikha
- MP Lau
- MP Miller
- MSM Jetten
- N Catalan
- N Fenner
- N Fromin
- ND Yan
- P Casper
- P Jäger
- P Kortelainen
- P Tezanos Pinto De
- R Cereghino
- R Conrad
- R Conrad
- R GĂłmez
- R Segers
- R Weinberger
- RW Tiner
- S Amalfitano
- S Manzoni
- S Qiu
- S Qiu
- S Qiu
- S Sobek
- S-R Xiang
- SC Maberly
- SE Evans
- SM Brothers
- T Akatsuka
- T Kalettka
- TC Winter
- V Peters
- W Armstrong
- W Merbach
- W Xiang
- WC Johnson
- WJ Xiao
- X Jiang
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Review
- Author
- Agard D.A.
- Akiyama S.K.
- Alagramam K.
- Albelda S.M.
- Allen S.P.
- Amon A.
- Amon A.
- Amon A.
- Amon A.
- Amrein K.E.
- Ang D.
- Aoki T
- Arebalo R.E.
- Arnason T.
- Baboshina O.V.
- Bailly V.
- Baker R.T.
- Baker R.T.
- Bamforth C.W.
- Bansal G.S.
- Barral Y
- Barrett T.B.
- Basco R.D.
- Beal R.
- Bell S.P.
- Bell S.P.
- Bercovich Z.
- Berg T.
- Biederer T.
- Blood C.H.
- Bohley P.
- Bohley P.
- Bohley P.
- Bohley P.
- Bohley P.
- Bone R.
- Bongers J.
- Bongers J.
- Bonifacino U.S.
- Borriss R.
- Boudreau N.
- Boudreau N.
- Brandts J.F.
- Bratovanova E.K.
- Brown J.L.
- Brown J.L.
- Brown K.
- Brown PL
- Bryant G.
- Buchner J.
- Bueno A.
- Bukau B.
- Busch H.
- Callebaut I.
- Cambillau C.
- Carillo S.
- Castronovo V.
- Celniker S.E.
- Chen P.
- Chen P.
- Cheng M.-Y.
- Cheng M.Y.
- Chiarini A.
- Clausell N.
- Clausell N.
- Cleary E.G.
- Coleman C.S.
- Condron R.
- Cook W. .
- Craik C.S.
- Creighton T.E.
- Creighton T.E.
- Creighton T.E.
- Creighton T.E.
- Cross F.R.
- D'Agrosa R.
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- Dahmann C.
- Dalton S.
- Darby N.J.
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- Dean R.T.
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- Dohmen R.J.
- Dowd M.K.
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- Eggers M.
- Emori Y.
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- Finley D.
- Fitch I.
- Foiani M.
- Forsburg S.L.
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- Freeh C.
- Frentzel S.
- Friedman D.I.
- Friedman H.
- Frydman J.
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- Gertler
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- Giulivi C.
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- Hartl F.-U.
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- Hayer-Hartl M.K.
- Hayes S.A.
- Hedstrom L.
- Hedstrom L.
- Hedstrom L.
- Heichman K.A.
- Hein C.
- Heinemann U.
- Heinemeyer W.
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- Heldin
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- Itzhaki L.S.
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- Jackson A.L.
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- Jakob U.
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- Jakubke H.-D.
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- Jämsä E.
- Kamitani
- Kasche V.
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- Kopp F.
- Kornitzer D.
- Koshland D.E. Jr.
- Kuwajima K.
- Kölling R.
- KĂĽhl P.
- KĂĽhne C.
- KĂĽntzel H.
- Lahav-Baratz S.
- Lamb J.R.
- Lanker S.
- LeBouton A.V.
- Lee H.K.
- Lee S.C.
- Leyser H.M.O.
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- Liang C.
- Lin C.Q.
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- Lisziewicz J.
- Loo S.
- Lopez-Fandino R.
- Luger K.
- Lydall D.
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- Molossi S.
- Morreau H.
- Morreau H.
- Morrison A.
- Murakami Y
- Murakami Y
- Mäher M.
- MĂĽcke M.
- MĂĽcke M.
- Nakai A.
- Nasmyth K.
- Nasmyth K.
- Nelson R.J.
- Neupert W
- Nguyen V.T.
- Nicholls A.
- Nilsson J.
- Nilsson K.G.I.
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- Olsen O.
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- Ordiz I.
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- Pacifici R.E.
- Palmer R.E.
- Paolini R.
- Parsell D.A.
- Pelham H.R.B.
- Perona J.J.
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- Rubin D.M.
- Rudolph R.
- Sadis S.
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- Sathyanarayana B.K.
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- Schellenberger V.
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- Seglen P.O.
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- Spence J.
- Stoller G.
- Stoller G.
- Sung P.
- Surana U.
- Surana U.
- Svensson B.
- Tanford C.
- Taylor
- The
- Thompson R.C.
- Toyo-Oka T.
- Tschesche H.
- Tschesche H.
- Tugendreich S.
- Tye B.-K.
- Tyers M.
- Ursic D.
- Valent Q.A
- van den Ussel P.R.L.A.
- Varga Z.
- Venekei I.
- Viitanen P.V.
- Wagner I.
- Waheed A.
- Wakarchuk W.W.
- Wan J.
- Wandrey C.
- Wang T.A.
- Warren R.A.J.
- Weissman J.S.
- Welfle K.
- Wenzel T.
- Wheatley D.N.
- Wheatley D.N.
- Wiebel F.F.
- Wong C.-H.
- Wrenn D.S.
- Yaffe M.B.
- Yaglom J.
- Yaglom J.
- Yamamoto A.
- Yamamoto A.
- Yang E.Y
- Yoon P.S.
- Yu H.
- Yusa T
- Zapun A.
- Zerner B.
- Zhang H.
- Zhou C.
- Zhou C.
- Zhu L
- Ziemienowicz A.
- Ziemienowicz A.
- Zwerschke W.
- Zwickl R
- Ă–zkaynak E.
- Publication venue
- 'Walter de Gruyter GmbH'
- Publication date
- Field of study
Protein folding in the cell
- Author
- A Colman
- A Holmgren
- A Holmgren
- A Mitraki
- AA Gatenby
- AA Laminet
- AJ Dorner
- AS Lee
- AS Lee
- BH Shieh
- C DeLuca-Flaherty
- C Dingwall
- C Georgopoulos
- C Georgopoulos
- CA Kumamoto
- CB Anfinsen
- CB Anfinsen
- CF Clarke
- CK Kassenbrock
- CL Pettey
- CS Copeland
- D Amir-Shipra
- D Haendler
- D Picard
- D Picard
- D Skowyra
- DA Parsell
- DG Bole
- DM Engman
- DM Schlossmann
- DN Collier
- DR Madden
- DRJ Macer
- DS Reading
- DTW Ng
- E Crooke
- E Degen
- EA Craig
- EA Craig
- EA Craig
- EA Craig
- EA Craig
- EA Craig
- EE Abola
- ER Price
- ER Schönbrunner
- ES Bochkareva
- F Rippmann
- FC Bernstein
- FC Dalman
- FC Dalman
- FU Hartl
- G Fischer
- G Spik
- G Wilderrecht
- GC Flynn
- GD Van Duyne
- GH Lorimer
- GJ Phillips
- GLE Koch
- GN Chandrasekhar
- H Murakami
- H-L Chiang
- HP Bachinger
- HRB Pelham
- HRB Pelham
- HRB Pelham
- I Sadler
- IA Wilson
- IG Haas
- J Ananthan
- J Buchner
- J Conde
- J Ellis
- J Heitman
- J Liu
- J Martin
- J Ostermann
- J Polaina
- J Pouyssegur
- JA Mendoza
- JA Rothblatt
- JC Edman
- JCA Bardwell
- JE Rothman
- JE Rothman
- JE Rothman
- JF Dice
- JF Dice
- JF Sambrook
- JP Vogel
- JR Subjeck
- JR Wetterau
- JS Bonifacino
- JS Brugge
- JS Brugge
- K Kuwajima
- K Lang
- K Liberek
- K Liberek
- K Normington
- KJ Howard
- KL Milarski
- KM Flaherty
- KM Flaherty
- KW Hasel
- LA Mizzen
- LL Randall
- LM Hendershot
- LM Hendershot
- LM Hendershot
- M Eilers
- M Eilers
- M Eilers
- M Geetha-Habib
- M Russel
- M Tropschug
- M Tropschug
- M Werner-Washburne
- M Zylicz
- MA Stammes
- MD Rose
- MF Flajnik
- MF Flajnik
- MJ Gething
- MJ Gething
- MJ Lewis
- MJ Lewis
- ML Lamantia
- MY Cheng
- MY Cheng
- N Kusukawa
- NJ Bulleid
- NJ Bulleid
- NM Lissin
- O Fayet
- OB Ptitsyn
- P Bird
- P Blount
- P Caroni
- P Csermely
- P Goloubinoff
- P Goloubinoff
- P Prevelige
- PE Danielson
- PE Scherer
- PJ Bjorkman
- PJ Kang
- PL Koser
- PS Kim
- PV Vitanen
- R Jaenicke
- R Zimmermann
- RA Laskey
- RA Mazzarella
- RA Mazzarella
- RA Roth
- RB Freedman
- RB Freedman
- RB Freedman
- RB Freedman
- RC Nicholson
- RI Morimoto
- RJ Deshaies
- RJ Ellis
- RJ Ellis
- RK Harrison
- RL Baldwin
- RL Hallberg
- RP Beckmann
- RW Hendrix
- S Lecker
- S Lindquist
- S Munro
- S Schneuwly
- S Sharma
- S Wickner
- SA Goff
- SJ Landry
- SL Morrison
- SL Schmid
- SL Schreiber
- SM Hemmingsen
- SM Hemmingsen
- SM Hurtley
- SW Fesik
- SW Michnick
- T Hayano
- T Leustek
- TE Creighton
- TG Chappell
- TG Chappell
- TH Lubben
- TH Lubben
- TK Van Dyk
- TW McMullin
- TW McMullin
- W Neupert
- W-J Chen
- WJ Chirico
- Y Hiromi
- Y Kozutsumi
- Y-J Jin
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study