Molecular Investigation and Phylogeny of Species of the \u3ci\u3eAnaplasmataceae\u3c/i\u3e Infecting Animals and Ticks in Senegal

Background: Our study aimed to assess the diversity of the species of Anaplasmataceae in Senegal that infect animals and ticks in three areas: near Keur Momar Sarr (northern region), Dielmo and Diop (Sine Saloum, central region of Senegal), and in Casamance (southern region of Senegal). Methods: A total of 204 ticks and 433 blood samples were collected from ruminants, horses, donkeys and dogs. Ticks were identified morphologically and by molecular characterization targeting the 12S rRNA gene. Molecular characterization of species of Anaplasmataceae infecting Senegalese ticks and animals was conducted using the 23S rRNA, 16S rRNA, rpoB and groEL genes. Results: Ticks were identified as Rhipicephalus evertsi evertsi (84.3%), Hyalomma rufipes (8.3%), Hyalomma impeltatum (4.9%), R. bursa (1.5%) and R. muhsamae (0.9%). The overall prevalence of Anaplasmataceae infection in ticks was 0.9%, whereas 41.1% of the sampled animals were found infected by one of the species belonging to this family. We identified the pathogen Anaplasma ovis in 55.9% of sheep, A. marginale and A. centrale in 19.4% and 8.1%, respectively, of cattle, as well as a putative new species of Anaplasmataceae. Two Anaplasma species commonly infecting ruminants were identified. Anaplasma cf. platys, closely related to A. platys was identified in 19.8% of sheep, 27.7% of goats and 22.6% of cattle, whereas a putative new species, named here provisionally “Candidatus Anaplasma africae”, was identified in 3.7% of sheep, 10.3% of goats and 8.1% of cattle. Ehrlichia canis and Anaplasma platys were identified only from dogs sampled in the Keur Momar Sarr area. Ehrlichia canis was identified in 18.8% of dogs and two R. e. evertsi ticks removed from the same sheep. Anaplasma platys was identified in 15.6% of dogs. Neither of the dogs sampled from Casamance region nor the horses and donkeys sampled from Keur Momar Sarr area were found infected by an Anaplasmataceae species. Conclusions: This study presents a summary of Anaplasmataceae species that infect animals and ticks in three areas from the northern, central and southern regions of Senegal. To our knowledge, our findings demonstrate for the first time the presence of multiple Anaplasmataceae species that infect ticks and domestic animals in Senegal. We recorded two potentially new species commonly infecting ruminants named here provisionally as Anaplasma cf. platys and “Candidatus Anaplasma africae”. However, E. canis was the only species identified and amplified from ticks. None of the other Anaplasmataceae species identified in animals were identified in the tick species collected from animals

La gestion post-accidentelle nucléaire pour les services déconcentrés du Ministère en charge de l'agriculture (rédaction d'un guide pour sa concrétisation opérationnelle)

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Bartonella bovis and Candidatus Bartonella davousti in cattle from Senegal

International audienceIn Senegal, domestic ruminants play a vital role in the economy and agriculture and as a food source for people. Bartonellosis in animals is a neglected disease in the tropical regions, and little information is available about the occurrence of this disease in African ruminants. Human bartonellosis due to Bartonella quintana has been previously reported in Senegal. In this study, 199 domestic ruminants, including 104 cattle, 43 sheep, and 52 goats were sampled in villages from the Senegalese regions of Sine Saloum and Casamance. We isolated 29 Bartonella strains, all exclusively from cattle. Molecular and genetic characterization of isolated strains identified 27 strains as Bartonella bovis and two strains as potentially new species. The strains described here represent the first Bartonella strains isolated from domestic ruminants in Senegal and the first putative new Bartonella sp. isolated from cattle in Africa. (C) 2016 Elsevier Ltd. All rights reserved

Anaplasma ovis infects sheep in Niger

International audienceAnaplasmosis due to Anaplasma ovis (A ovis) is an infection spread in small ruminants mostly in the tropical and subtropical region. This neglected disease which often induces a subclinical infection can be severe in case of co-infection with other pathogens. In the Republic of Niger, small ruminant breeding contributes for a large extent to the national economy; however, little is known about pathogens that infect sheep in this region. In the present study, 33 sheep blood samples were collected near the village of Madama in the north of the country. Molecular analysis for the investigation of the presence of Anaplasmataceae species infection was conducted targeting three genes, the 23S rRNA, 16S rRNA and the rpoB genes. Results showed that 23/33 (69.7%) of the sheep were infected by A. ovis. This result is the first molecular description of A. ovis in Niger

Molecular investigation and phylogeny of species of the Anaplasmataceae infecting animals and ticks in Senegal

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Leptospirosis, one neglected disease in rural Senegal

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Great diversity of Piroplasmida in Equidae in Africa and Europe, including potential new species

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