51 research outputs found

    Dummy <I>Yersinia pestis </I>Strains: Selection Criteria, Usage Guidelines

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    Objective of the work was to develop selection criteria for the dummy Y. pestis strains, as well as principles of the setting-up a panel and its application for practicing laboratory and differential diagnostics of plague. Studied were the RF regulations, statutory documents and methodological recommendations on the laboratory diagnostics of plague and safety of works with microorganisms; training courses for specialists to qualify for work with the agents of particularly dangerous infections. Research method: analytical. Consequently, established were the term for “dummy strain”; selection criteria for the Y. pestis strains used in the practical course within the frames of the training programme “Microbiology and Laboratory Diagnosis of Plague”; and algorithm of the course application in view of biological risk mitigation during the process of education

    Atomic force microscopy in polymeric chemistry’s studies

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    This work was supported by the RSF (project No. 18-19-00453)

    An atomic force microscopy study of hybrid polymeric membranes: Surface topographical analysis and estimation of pore size distribution

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    © 2016, Pleiades Publishing, Ltd.The surface morphology of polymeric membranes as organic–inorganic block copolymers has been studied by atomic force microscopy (AFM). These hybrid block copolymers have been obtained by the polyaddition of toluene 2,4-diisocyanate to macroinitiators and the addition of oligomeric polyhedral оctaglycidyl silsesquioxane (Gl-POSS) as a bulky branching agent in a concentration range of 0.1–15 wt %. The AFM study of the morphology made it possible to determine the main roughness parameters and to perform topographical analysis of the surface of the polymers. The pore size distribution was evaluated by histogrammic approximation with the use of the Gauss distribution. The hypothesis of the normalcy of distribution of the experimental sample of pore diameters was confirmed by a combined criterion and Pearson’s chi-square goodness-of-fit test. The effect of the Gl-POSS concentration on the surface morphology and the microstructure of the organic–inorganic polymeric membranes was found to be nonmonotonic over the test concentration range

    Synthesis and properties of novel polyurethanes based on amino ethers of boric acid for gas separation membranes

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    © The Royal Society of Chemistry. Herein we present the structural and mechanical properties of polyurethanes synthesized from amino ethers of boric acid for gas separation. The polymers were characterized by light scattering methods, conductivity measurements, thermal gravimetric analysis, Fourier transform infrared spectroscopy, and atomic force microscopy. Additionally, the permeability of ammonia and carbon dioxide, as well as the selectivity for their diffusion and resultant impurity are presented. The results illustrate the steric hindrance, resulting in a branched architecture borate formation, leads to intermolecular complexation which may assist the polymer in ammonia diffusion selectivity

    Surface structuring features of polymeric membranes based on chitosan according to atomic force microscopy

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    С целью выявления механизмов структурообразования модифицированных полимерных мембран на основе хитозана на каждом этапе модификации с помощью метода атомно-силовой микроскопии изучена серия промежуточных образцов.For to reveal surface structuring mechanisms of modified polymeric membranes based on chitosan, a series of intermediate samples was studied using atomic force microscopy at each modification stage.Работа выполнена при финансовой поддержке Российского научного фонда в рамках проекта № 15-19-10057

    PLAGUE INFECTION SIMULATING IN CASE OF INOCULATION WITH AVIRULENT YERSINIA PESTIS STRAINS

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    Biological method of investigation is specified for the laboratory diagnostics of plague. Mastering of this method by the trainees within the frames of further vocational education is associated with the use of avirulent Yersinia pestis strains and vaccine Y. pestis strain EV line, which while providing safety does not allow for typical pathomorphological pattern on biomodels, as well as for isolation of microorganisms from internal organs. Objective of the study is to select avirulent Yersinia pestis strains and to conduct comparative analysis of the simulation techniques for plague on biomodels. Materials and methods. Utilized were Y. pestis strains. Virulence was evaluated both, in vitro (polymerase chain reaction) and in vivo (LD50 for white mice). Results and conclusions. Set forward have been avirulent Y. pestis strains, prospective in terms of mastering biological method of laboratory diagnostics of plague, and means of their application for simulating plague in biomodels. The designed approach allows for exercising biological methods of plague investigation to the fullest extent, enhancing biological safety of practical studies and reducing the time line for isolation and accumulation of pure bacterial culture

    Chronometry as a Method to Assess the Formation of Skills Required for Work with the Agents of Particularly Dangerous Infections in the Course of Professional Retraining

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    The aim of the study is to assess how successfully the trainees obtain skills of post mortem examination of laboratory animals, with strict observance of the rules of safe work with pathogenic biological agents of I-II groups, during their training at professional retraining courses. Individual continuous chronometry is offered as the assessment method. The obtained results can be used for planning of practical training, drafting the time-table, standardization of work of instructors
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