Temporal and geographical distribution of the strains from the ten DENV-1 clusters.

<p>3,108 DENV-1 envelope sequences, obtained from the European Molecular Biology Laboratory (EMBL) Nucleotide Sequence Database in April 2014, were aligned with the DENV-1 Lao sequences obtained in this study. A tree was built (<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0006203#pntd.0006203.g004" target="_blank">Fig 4</a>) and ten clusters (bootstrap >70) containing the strains from this study were identified. In order to visualize the circulation of all the strains from these different clusters, we plotted each strain according to time of collection and location. Each of the 10 clusters is displayed in a different color: yellow for the strains from cluster # 1, light green for cluster #2, red for cluster #3, blue for cluster #4, dark green for cluster #5, medium green for cluster # 6, pink for cluster #7, grey for cluster #8, light orange for cluster #9 and dark orange for cluster #10. The list of all the strains included in each cluster is provided as <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0006203#pntd.0006203.s007" target="_blank">S6 Table</a>.</p

Seasonality of recruited patients and confirmed dengue cases at the three sites.

<p>Total: recruited patients. Conf dengue: confirmed dengue cases, Average Temp: monthly mean temperature.</p

<i>Dengue virus</i> infection and serotypes detected per site and per year.

<p><i>Dengue virus</i> infection and serotypes detected per site and per year.</p

<i>Dengue virus</i> serotypes distribution over time at the three sites.

<p>Available sequences for each site are indicated with circles for complete CDS and with crosses for envelope on the top of the histograms, including sequences previously published from same locations [<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0006203#pntd.0006203.ref023" target="_blank">23</a>].</p