DESIGN AND EVALUATION OF DOMPERIDONE SUBLINGUAL TABLETS

Objective: The aim of this work was to enhance the bioavailability of poorly soluble, anti-emetic drug; domperidone (DMP) having a poor oral bioavailability (13-17%) due to extensive first pass metabolism. The goal of this study was achieved through solubilization of DMP using solid dispersion technology followed by incorporation of solid dispersions into sublingual tablets to bypass pre-systemic metabolism.Methods: Solid dispersions of DMP with Pluronic F-68 were prepared in different weight ratios by fusion method and they were evaluated for their in vitro dissolution rate to select the best ratio for final formulation. Then, solid dispersions were formulated into sublingual tablets in combination with various soluble excipients. Sublingual tablets were prepared by direct compression technique and evaluated for their physical properties, in vitro dissolution rate and kinetics of drug release. The best formulae were selected for in vivo studies in rabbits in comparison with marketed oral tablets; Motinorm®.Results: Solid dispersions of DMP with Pluronic F-68 in a weight ratio of 1:7 (w/w) showed the highest dissolution rate and were selected for sublingual tablets formulation. Sublingual tablets formulae S16 (containing Fructose and 10% w/w Ac-Di-Sol) and S20 (containing Fructose and 10% w/w Explotab) showed the best results and were selected for in vivo studies in rabbits. The selected formulae showed marked enhancement of DMP bioavailability compared with the commercial oral tablets; Motinorm®, with relative bioavailability values of 432.49±10.13% and 409.32±11.59 % for S16 and S20, respectively.Conclusion: The results confirmed that sublingual tablets were an effective tool for DMP delivery with marked enhancement of bioavailability.Keywords: Domperidone, Solubility, Solid dispersions, Sublingual tablets, First-pass metabolism, Bioavailabilit

Long-term protection of hepatitis B vaccination among Egyptian children

Background: Hepatitis B Vaccination is the most effective way to prevent transmission of hepatitis B virus (HBV). Objective: to detect the long-term immunogenicity of the vaccine in Egyptian children after five and ten years of vaccination. Methods: Two hundreds healthy children were recruited. They were divided into two groups according to their age. Group A included 100 child, around 6 years old, vaccinated 5 years ago. Group `B` included 100 child, around 11 years old, vaccinated 10 years ago. Hepatitis B surface antibody (HBsAb) titre was tested, booster dose of the vaccine was given to children whose HBsAb was < 10 mIU/ml, then one and half month later, they were retested for HBsAb to evaluate the response. Results: Both groups had a wide range of HBsAb (2-1000 mlU/ml), and there was a significant difference in the level of the two groups. Our data proves the decline of antibody titre with time. In group A, 19 children needed a booster dose, 14 of them were vaccinated, and 10 were retested after one and half month. The results showed that 9 (90%) responded by increased level of HBsAb, with six (66.6%) showing an adequate response. In group B, 52 children had antibody titre < 10, 48 of them were vaccinated and 34 were retested one and half months later. Two out of the 34 did not respond and 32 (94.2%) responded by an increase in the antibody titre. Of those who responded, 19 had adequate response (HBsAb ≥ 100) and 13 had hypo-response (HBsAb = 10 -100). Eighty percent (80%) of boys versus 51.7% of girls responded adequately. Conclusion: Hepatitis B vaccine is an effective and successful way for preventing HBV infection. No need for booster dose at least for 5 years after vaccination .Keywords: HBV- HB vaccine- long term immunityEgypt J Pediatr Allergy Immunol 2011;9(1):35-4

On modeling two immune effectors two strain antigen interaction

In this paper we consider the fractional order model with two immune effectors interacting with two strain antigen. The systems may explain the recurrence of some diseases e.g. tuberculosis (TB). The stability of equilibrium points are studied. Numerical solutions of this model are given. Using integer order system the system oscillates. Using fractional order system the system converges to a stable internal equilibrium. Ulam-Hyers stability of the system has been studied

Sex Hormones Changes in Blood and Their Effect on Fecundity of African Catfish (Clarias gariepinus Burchell, 1822) after Being Injected with Different Doses of Human Chorionic Gonadotropin (HCG) Hormone

This study was conducted to investigate the effect of different dosesinjection of human chorionic gonadotropin (HCG) hormone on fecundityand serum sex hormones (FSH, LH, estrogen (E2), progesterone (P4),testosterone (T)) of African catfish (Clarias gariepinus). African catfishspawners were intermuscularly injected with different doses of HCG(500, 1500, 3000, 6000 IU/kg female), and group is not injected as acontrol; males were injected at half the female dose. The results showedthat, fish group injected by 6000 IU HCG/ kg female had the highestgonadsomatic index, absolute fecundity and relative fecundity, while,the lowest value of absolute fecundity and relative fecundity wererecorded with 500 IU HCG/ kg female. The group injected with thehighest amount of HCG (6000 IU/ kg female) recorded the lowest valuefrom egg diameter, while the highest egg diameter was observed in 500IU HCG/ kg female. In females, the group injected with 6000 IU HCG/kg female reflected the lowest level of FSH and the highest level of LHand the highest level of P4 compared to other treatments. Level of Trecorded the highest level with 1500 IU HCG/ kg female. The controlgroup reflected the highest level of FSH and E2, while the control groupreflected the lowest level of T and P4 level. In males, serum FSH, LH,P4 and E2 in male groups injected with HCG were relatively higher thanthose recorded in the control group. The highest level of T was recordedin treatment injected with the highest dose of HCG and decreased inother treatments until recorded the lowest level of T in the controlgroup. It was observed, HCG hormone has successfully and accelerateinduced spawning in African catfish (Clarias gariepinus) and increasedin reproductive performance with the increase in HCG dosage and ascompared to group not injected

Analgesic Effect of Intra-Articular Dexamethasone versus Fentanyl added as an adjuvant to Bupivacaine for Postoperative Pain Relief in Knee Arthroscopic Surgery

Background: Knee arthroscopy is usually associated with a variable degree of pain ranging from moderate-to-severe pain in about 70% of patients. Objective: This trial was designed to assess the efficacy of intra-articular administration of dexamethasone versus fentanyl as adjuncts to bupivacaine in patients undergoing arthroscopic knee surgery. Patients and methods: Eighty-nine patients of either sex were enrolled in this study. The patients were randomly divided into three equal groups. Group F that received intra-articular (IA) injection of 1 μg/kg fentanyl (In 2 ml saline) added to 18 ml of 0.25% bupivacaine, group D, which received IA injection of 8 mg (2 ml) dexamethasone added to 18mL of 0.25% bupivacaine and group S that received IA injection of 2 ml normal saline added to 18 mL of 0.25% bupivacaine. Results: The time required for the first request of analgesia in group F, group D, and group S was 5.7 ± 0.7 vs 4.5 ± 0.5 vs 3.3 ± 0.5 hours respectively. There were significant differences between both treatment groups and the control group (p < 0.001) and in between both treatment groups (p < 0.001) in favor of group F. There was a significantly lower median visual analogue score in group F when compared to group D and S at 6 hours (p = 0.006 & 0.01, respectively), 12 hours (p < 0.001 & < 0.001, respectively), and 18 hours (p = 0.003 & 0.007, respectively) postoperatively. Conclusion: The addition of fentanyl or dexamethasone to IA bupivacaine in knee arthroscopic surgery provided a better quality of analgesia with less consumption of systemic analgesics without significant adverse effects

Flavonoid-coated gold nanoparticles as efficient antibiotics against gram-negative bacteria—evidence from in silico-supported in vitro studies

Flavonoids are a class of bioactive plant-derived natural products that exhibit a broad range of biological activities, including antibacterial ones. Their inhibitory activity toward Gram-positive bacterial was found to be superior to that against Gram-negative ones. In the present study, a number of flavonoid-coated gold nanoparticles (GNPs) were designed to enhance the antibacterial effects of chrysin, kaempferol, and quercetin against a number of Gram-negative bacteria. The prepared GNPs were able to conjugate to these three flavonoids with conjugation efficiency ranging from 41% to 80%. Additionally, they were able to exert an enhanced antibacterial activity in comparison with the free flavonoids and the unconjugated GNPs. Quercetin-coated GNPs were the most active nano-conjugates and were able to penetrate the cell wall of E. coli. A number of in silico experiments were carried out to explain the conjugation efficiency and the antibacterial mechanisms of these flavonoids as follows: (i) these flavonoids can efficiently bind to the glutathione linker on the surface of GNPs via H-bonding; (ii) these flavonoids, particularly quercetin, were able to increase the bacterial membrane rigidity, and hence decrease its functionality; (iii) these flavonoids can inhibit E. coli’s DNA gyrase (Gyr-B) with IC(50) values ranging from 0.9 to 3.9 µM. In conclusion, these bioactive flavonoid-based GNPs are considered to be very promising antibiotic candidates for further development and evaluation

Antiviral activity of chitosan nanoparticles for controlling plant-infecting viruses

Chitosan nanoparticles (ChiNPs) are a potentially effective means for controlling numerous plant diseases. This study firstly describes the antiviral capabilities of ChiNPs to control plant viral diseases compared to its bulk form. Bean yellow mosaic virus (BYMV) was used as a model plant virus affecting faba bean plants and many other legumes. The antiviral effectiveness of ChiNPs and chitosan were evaluated as a curative application method, using six dosage rates (50, 100, 200, 250, 300 and 400 mg/L). Results indicated that ChiNPs curatively applied 48 h post virus inoculation entirely inhibit the disease infectivity and viral accumulation content at 300 mg/L and 400 mg/L. The virus titre was greatly alleviated within the plant tissues by 7.71% up to100% depending on ChiNP dosage rates. However, chitosan used in its bulk-based material form revealed a relatively low to an intermediate reduction in virus infectivity by 6.67% up to 48.86%. Interestingly, ChiNPs affect the virus particle’s integrity by producing defective and incomplete BYMV viral particles, defeating their replication and accumulation content within the plant tissues. Simultaneously, ChiNP applications were appreciably shown to promote the pathogenesis-related (PR-1) gene and other defence-related factors. The mRNA of the PR-1 gene was markedly accumulated in treated plants, reaching its maximum at 400 mg/L with 16.22-fold relative expression change over the untreated control. Further, the total phenol dynamic curve was remarkably promoted for 30 days in response to ChiNP application, as compared to the untreated control. Our results provide the first report that chitosan-based nanomaterials have a superior effect in controlling plant viruses as an antiviral curing agent, suggesting that they may feasibly be involved in viral disease management strategies under field conditions without serious health concerns and environmental costs. Significance: • Our findings show that chitosan nanoparticles have a powerful curing antiviral activity against BYMV disease. These findings open the door for the use of eco-friendly nano-based tools in controlling numerous plant viruses. The use of eco-friendly nano-based materials could result in a successful integrative control strategy for plant viruses under field conditions, negating the need for the conventional measure used to control most of the insect-transmitted plant viruses, that is insecticide application against vector insects

Effectiveness of Educational Nursing Strategies on Nurse’s Knowledge and Skills regarding Traumatic Head Injury in Children

Traumatic head injury is a pressing public health problem leading to disability and death in children and adolescents worldwide. Aim: This study aimed to assess the effectiveness of educational nursing strategies on nurse’s knowledge and skills regarding traumatic head injury in children. Setting: The study was conducted in the Paediatric Intensive Care Unit and Emergency Department at Birket El Sabah Central Hospital, Menoufia Governorate, Egypt. Methods:  A quasi-experimental design was carried out for this study (pre/post & follow-up). A convenience sample of 40 nurses provided direct nursing care to the children with traumatic head injury in the above-mentioned setting was selected from February to October 2019. Two tools were used for data collection, tool one: A structured interviewing questionnaires, it consisted of 7 sections to assess nurses' knowledge. Tool two: An observational checklist to assess the nurses’ practice of the Glasgow coma scale. Results: The results of this study revealed that there was a high statistical significance difference between pre, post and follow up tests regarding nurses’ knowledge and skills of children with traumatic brain injury at 1% level of statistical significance. Conclusion, Implementation of educational nursing strategies improved nurses' knowledge and skills while managing children with traumatic head injury. Recommendation, Continuous educational programs should be scheduled on a constant base for nurses' about traumatic brain injury to enhance children’s quality of care and improve their outcome. Keywords: Educational nursing strategies, Nurses, Knowledge, Skills, Children, Traumatic head injury DOI: 10.7176/JHMN/69-12 Publication date: December 31st 201