Additional file 2 of Lineage-specific, fast-evolving GATA-like gene regulates zygotic gene activation to promote endoderm specification and pattern formation in the Theridiidae spider

Additional file 2: Table S1. List of DEG candidates identified by comparative transcriptome analysis of c versus i/p cells from stage-3 embryo (comparison I). Table S2. List of DEG candidates identified by comparative transcriptome analysis of p versus c/i cells from stage-3 embryo (comparison II). Table S3. List of DEG candidates identified by comparative transcriptome analysis of i versus c/p cells from stage-3 embryo (comparison III). Table S4. List of DEG candidates indentified by comparative transcriptome analysis of c versus p cells from stage-4 embryo. Table S5. List of DEG candidates identified by comparative transcriptome analysis of c versus p cells from early stage-5 embryo

Additional file 12 of Lineage-specific, fast-evolving GATA-like gene regulates zygotic gene activation to promote endoderm specification and pattern formation in the Theridiidae spider

Additional file 12: Table S16. Primers and cDNA clones used for RNAi and in situ hybridization

Additional file 5 of Lineage-specific, fast-evolving GATA-like gene regulates zygotic gene activation to promote endoderm specification and pattern formation in the Theridiidae spider

Additional file 5: Movie S2. Time-lapse video showing phenotypes gfp, g26874, g7720, and g4238 pRNAi embryos. Time after egg laying (AEL) and time after start of recording are indicated. Movie starts are adjusted by the timing of blastoderm formation. Some of the embryos are the same as those shown in Fig. 3A. Scale bar, 100 µm

Additional file 7 of Lineage-specific, fast-evolving GATA-like gene regulates zygotic gene activation to promote endoderm specification and pattern formation in the Theridiidae spider

Additional file 7: Table S7. List of bioinformatic resources used for phylogenetic characterization of Fuchi and other GATA family members in spiders. Table S8. Amino acid sequence alignment and classification of GATA family members from spiders and other metazoans

Additional file 15 of Lineage-specific, fast-evolving GATA-like gene regulates zygotic gene activation to promote endoderm specification and pattern formation in the Theridiidae spider

Additional file 15. Multi-fasta format file of 168 sequences (63 amino acid sites) used for the second ML analysis

Additional file 10 of Lineage-specific, fast-evolving GATA-like gene regulates zygotic gene activation to promote endoderm specification and pattern formation in the Theridiidae spider

Additional file 10: Table S12. Data from comparative analysis of read counts in extracted ATAC-seq peaks between fuchi pRNAi and wild-type embryos at stage 3 using edgeR. Table 13. List of differential ATAC-seq peaks between fuchi pRNAi and wild-type embryos at stage 3 (FDR < 0.05)

Additional file 11 of Lineage-specific, fast-evolving GATA-like gene regulates zygotic gene activation to promote endoderm specification and pattern formation in the Theridiidae spider

Additional file 11: Table S14. Data from comparative analysis of read counts in extracted ATAC-seq peaks between Pt-hh pRNAi and wild-type embryos at stage 3 using edgeR. Table S15. List of differential ATAC-seq peaks between Pt-hh pRNAi and wild-type embryos at stage 3 (FDR < 0.05)

Additional file 13 of Lineage-specific, fast-evolving GATA-like gene regulates zygotic gene activation to promote endoderm specification and pattern formation in the Theridiidae spider

Additional file 13. Multi-fasta format file of 212 protein sequences used for molecular phylogenetic characterization

Additional file 14 of Lineage-specific, fast-evolving GATA-like gene regulates zygotic gene activation to promote endoderm specification and pattern formation in the Theridiidae spider

Additional file 14. Multi-fasta format file of 118 sequences (75 amino acid sites) used for the first ML analysis

Additional file 4 of Lineage-specific, fast-evolving GATA-like gene regulates zygotic gene activation to promote endoderm specification and pattern formation in the Theridiidae spider

Additional file 4: Table S6. Summary of a pilot pRNAi screen of 19 selected genes