Procedure for extraction of ellagic acid from lady`s mantles (Alchemilla vulgaris L.) RS-P-2017/0289.

Pronalazak se odnosi na nov postupak ekstrakcije elaginske kiseline iz herbe biljne vrste gospin plašt (Alchemilla vulgaris L.). Postupak ekstrakcije pokazuje neočekivanu selektivnost za elaginsku kiselinu, dok je sadržaj drugih jedinjenja ispod granica detekcije. Ispitivanja ekstrakta A. vulgaris pokazuju zadovoljavajuću antioksidativnu aktivnost, a određen je i sadržaj ukupnih flavonoida. Dobijeni ekstrakt herbe A. vulgaris može biti primenjen kao prirodni aditiv u prehrambenoj, kozmetičkoj i farmaceutskoj industriji, jer ima jak antioksidativni i zaštitni efekat.Решење о признању патента 990 број 2019/8183-П-2017/0289 од 14.05.2019. објава патента: Гласник интелектуалне својине 5/2019, Завод за интелектуалну својину, Република Србија. (RS 58657 B1

Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles

Black locust flower extract contains various polyphenols and their glucosides contribute to the potential health benefits. After intake of these bioactive compounds and passage through the gastrointestinal tract, their degradation can occur and lead to a loss of biological activity. To overcome this problem, the bioactive compounds should be protected from environmental conditions. This study aimed to encapsulate the black flower extract in the microparticles based on biodegradable polysaccharides, alginate, and chitosan. In the extract, the total antioxidant content was found to be 3.18 ± 0.01 g gallic acid equivalent per 100 g of dry weight. Also, the presence of lipids (16), phenolics (27), organic acids (4), L-aspartic acid derivative, questinol, gibberellic acid, sterol, and saponins (2) was confirmed using the UHPLC–ESI–MS analysis. In vitro assays showed that the extract has weak anti-α-glucosidase activity and moderate antioxidant and cytotoxic activity against the HeLa cell line. The extrusion method with secondary air flow enabled the preparation of microparticles (about 270 μm) encapsulated with extract. An encapsulation efficiency of over 92% was achieved in the alginate and alginate–chitosan microparticles. The swelling study confirmed a lower permeability of alginate–chitosan microparticles compared with alginate microparticles. For both types of microparticles, the release profile of antioxidants in the simulated gastrointestinal fluids at 37 °C followed the Korsmeyer–Peppas model. A lower diffusion coefficient than 0.5 indicated the simple Fick diffusion of antioxidants. The alginate–chitosan microparticles enabled a more sustained release of antioxidants from extract compared to the alginate microparticles. The obtained results indicated an improvement in the antioxidant activity of bioactive compounds from the extract and their protection from degradation in the simulated gastric conditions via encapsulation in the polymer matrixes. Alginate–chitosan showed slightly slower cumulative antioxidant release from microparticles and better antioxidant activity of the extract compared to the alginate system. According to these results, alginate–chitosan microparticles are more suitable for further application in the encapsulation of black locust flower extract. Also, the proposed polymer matrix as a drug delivery system is safe for human use due to its biodegradability and non-toxicity

Hemijski sastav i biološka aktivnost ekstrakta semena šljive

The aim of this paper was to estimate the biological activity of the plum seed extract and to define the chemical composition by using the ESI-MS method. During the investigation of the antioxidant activity, the extract showed a better ability to inhibit DPPH radicals compared with amygdalin standard. The results of the antimicrobial study indicate that the extract has a greater effect on Gram-negative bacteria compared with amygdalin. Gram-positive bacteria and fungi remained resistant in both cases. Unlike amygdalin, the extract showed a greater antimicrobial activity against the same microbiological strains. Amygdalin was isolated from the extract before the investigation of antiproliferative activity against MDA-MB-361, MDA-MB-453, HeLa and LS174 cell lines using MTT assay. Unlike the MDA-MB-453 cell line, the activity of standard and isolated amygdalin was not observed at the concentrations lower than 400 μg cm-3 on MDA-MB-361 cell line. The isolated amygdalin has not shown the activity in other cell lines. The antiproliferative activity of amygdalin standard was also noticed on HeLa and LS-174 cell lines at the concentrations of 333.27 and 333.17 μg cm-3, respectively.Cilj ovog rada bila je procena biološke aktivnosti ekstrakta semena šljive i definisanje hemijskog sastava primenom ESI-MS metode. Tokom ispitivanja antioksidativne aktivnosti, ekstrakt je pokazao bolju sposobnost inhibiranja DPPH radikala u poređenju sa standardom amigdalina. Rezultati antimikrobne studije pokazali su da ekstrakt ima veći uticaj na rast Gram-negativnih bakterija u odnosnu na amigdalin. Gram-pozitivne bakterije i gljivice ostale su rezistentne u oba slučaja. Za razliku od amigdalina, ekstrakt je pokazao veću antimikrobnu aktivnost na iste sojeve mikroorganizama. Amigdalin je izolovan iz ekstrakta pre ispitivanja antiproliferativne aktivnosti na MDA-MB-361, MDA-MB-453, HeLa i LS174 ćelijskim linijama upotrebom MTT testa. Za razliku od MDA-MB-453 ćelijskih linija, aktivnost standarda i izolovanog amigdalina nije uočena na MDA-MB-361 ćelijskim linijama pri koncentracijama nižim od 400 μg cm-3. Izolovani amigdalin nije pokazao aktivnost na ostalim ćelijskim linijama. Antiproliferativna aktivnost standarda amigdalina uočena je i na HeLa i LS-174 ćelijskim linijama pri koncentracijama 333.27 i 333.17 μg cm-3, respektivno

Postupak primene termoosetljivih hidrogelova za modifikovano oslobađanje elaginske kiseline

Pronalazak se odnosi na novu primenu hidrogelova poli(N-izopropilakrilamid-ko-2-hidroksipropilmetakrilata), p(NIPAM-HPMet)-a i poli(N-izopropilakrilamida), p(NIPAM)-a, kao nosača za modifikovano oslobađanje elaginske kiseline i ekstrakta Alchemilla vulgaris L. sa visokim sadržajem elaginske kiseline. Analiza FTIR spektara homopolimera p(NIPAM)-a i kopolimera p(NIPAM-HPMet)-a sa uklopljenim standardom elaginske kiseline i ekstraktom Alchemilla vulgaris L., ukazuju na formiranje intermolekulskih vodoničnih veza između lanaca homopolimera, tj. kopolimera, s jedne strane i elaginske kiseline, s druge strane. Hidrogelovi p(NIPAM) i p(NIPAM-HPMet) su se pokazali kao pogodni nosači elaginske kiseline, jer omogućavaju njeno modifikovano oslobađanje

Mineral Composition of Subcritical Water Extracts of Saccorhiza Polyschides, a Brown Seaweed Used as Fertilizer in the North of Portugal

The present work aimed at studying Saccorhiza polyschides extracts obtained by subcritical water extraction as a potential source of essential macro and trace elements, aiming for its potential application as a biofertilizer. The mineral composition, as well as sulfate, chlorine and iodine, total organic matter, and total nitrogen content, were determined on the extracts obtained from seaweeds harvested during low tide at the northern Portuguese coast. The selected parameters are important for a biofertilizer. Among the macronutrients, the most abundant was K (15.7 ± 0.2 g/L), followed by Na (5.46 ± 0.11 g/L), S (1.52 ± 0.06 g/L), Ca (1.09 ± 0.11 g/L), and Mg (1.02 ± 0.08 g/L). Several important micronutrients (Zn, B, Cl, P, Mo, V, Se, and I) have also been found in the extracts. The total organic matter was 34.1 ± 0.3 g/L. The extracts present low levels of toxic compounds such as Ni, Cd, and Pb. Considering the composition of the obtained extracts, these can find application in the development of fertilization products. The composition of subcritical water extracts of S. polyschides suggests that they may have important characteristics as a biofertilizer and can be an option in biofortification experiments with essential nutrients. The method can be easily scaled up which makes it attractive for agricultural applications.info:eu-repo/semantics/publishedVersio

Possible use of hydropower potential of cooling water at the thermal power plant

U radu je prikazana mogućnost korišćenja hidroenergetskog potencijala rashladne vode termoelektrane sa otvorenim sistemom hlađenja. Rashladna voda otiče gravitaciono do recipijenta - reke, zbog čega se u periodu nižih vodostaja javlja pad koji je moguće iskoristiti u maloj hidoelektrani. Takva hidroelektrana radi sa promenljivim padom i približno konstantnim protokom, i to samo u periodima kada radi i termoelektrana. Koncepcija je prikazana na primeru male hidroelektrane koja će koristiti hidroenergetski potencijal rashladne vode TE 'Nikola Tesla B'. Određeni su ekonomski pokazatelji ove hidroelektrane i ocenjeno je povećanje profitabilnosti imajući u vidu da postrojenje može biTI ostvareno u okvira Clean Development Mechanism (CDM) projekta, u skladu sa Kjoto protokolom. Dobijeni rezultati pokazuju da je projekat ekonomski prihvatljiv, a moguće ga je ostvariti sa standardnim hidroturbinama dostupnim na tržištu.Possibility of using hydropower potential of cooling water at the thermal power plant with open cooling system is presented in the paper. Cooling water flows gravitationally to the recipient - the river. In the periods of low water levels additional head occurs which could be used in small hydropower plant. Such a hydropower plant will operate only in periods when thermal power plant operates, with more or less constant flow and head that significantly changes in time. Small hydropower plant that uses cooling water of Thermal Power Plant 'Nikola Tesla B' is presented in the paper. The economic benefits of the HPP are calculated. The increase of profitability is assessed, bearing in mind that the plant would be realized as the Clean Development Mechanism project according to the Kyoto protocol. The obtained results show that the project is economically attractive, and it can be carried out with standard matured solutions of hydro turbines available at the market

Mogućnost korišćenja hidroenergetskog potencijala vode za hlađenje termoelektrana

Possibility of using hydropower potential of cooling water at the thermal power plant with open cooling system is presented in the paper. Cooling water flows gravitationally to the recipient - the river. In the periods of low water levels additional head occurs which could be used in small hydropower plant. Such a hydropower plant will operate only in periods when thermal power plant operates, with more or less constant flow and head that significantly changes in time. Small hydropower plant that uses cooling water of Thermal Power Plant 'Nikola Tesla B' is presented in the paper. The economic benefits of the HPP are calculated. The increase of profitability is assessed, bearing in mind that the plant would be realized as the Clean Development Mechanism project according to the Kyoto protocol. The obtained results show that the project is economically attractive, and it can be carried out with standard matured solutions of hydro turbines available at the market.U radu je prikazana mogućnost korišćenja hidroenergetskog potencijala rashladne vode termoelektrane sa otvorenim sistemom hlađenja. Rashladna voda otiče gravitaciono do recipijenta - reke, zbog čega se u periodu nižih vodostaja javlja pad koji je moguće iskoristiti u maloj hidoelektrani. Takva hidroelektrana radi sa promenljivim padom i približno konstantnim protokom, i to samo u periodima kada radi i termoelektrana. Koncepcija je prikazana na primeru male hidroelektrane koja će koristiti hidroenergetski potencijal rashladne vode TE 'Nikola Tesla B'. Određeni su ekonomski pokazatelji ove hidroelektrane i ocenjeno je povećanje profitabilnosti imajući u vidu da postrojenje može biTI ostvareno u okvira Clean Development Mechanism (CDM) projekta, u skladu sa Kjoto protokolom. Dobijeni rezultati pokazuju da je projekat ekonomski prihvatljiv, a moguće ga je ostvariti sa standardnim hidroturbinama dostupnim na tržištu

Possible use of hydropower potential of cooling water at the thermal power plant

U radu je prikazana mogućnost korišćenja hidroenergetskog potencijala rashladne vode termoelektrane sa otvorenim sistemom hlađenja. Rashladna voda otiče gravitaciono do recipijenta - reke, zbog čega se u periodu nižih vodostaja javlja pad koji je moguće iskoristiti u maloj hidoelektrani. Takva hidroelektrana radi sa promenljivim padom i približno konstantnim protokom, i to samo u periodima kada radi i termoelektrana. Koncepcija je prikazana na primeru male hidroelektrane koja će koristiti hidroenergetski potencijal rashladne vode TE 'Nikola Tesla B'. Određeni su ekonomski pokazatelji ove hidroelektrane i ocenjeno je povećanje profitabilnosti imajući u vidu da postrojenje može biTI ostvareno u okvira Clean Development Mechanism (CDM) projekta, u skladu sa Kjoto protokolom. Dobijeni rezultati pokazuju da je projekat ekonomski prihvatljiv, a moguće ga je ostvariti sa standardnim hidroturbinama dostupnim na tržištu.Possibility of using hydropower potential of cooling water at the thermal power plant with open cooling system is presented in the paper. Cooling water flows gravitationally to the recipient - the river. In the periods of low water levels additional head occurs which could be used in small hydropower plant. Such a hydropower plant will operate only in periods when thermal power plant operates, with more or less constant flow and head that significantly changes in time. Small hydropower plant that uses cooling water of Thermal Power Plant 'Nikola Tesla B' is presented in the paper. The economic benefits of the HPP are calculated. The increase of profitability is assessed, bearing in mind that the plant would be realized as the Clean Development Mechanism project according to the Kyoto protocol. The obtained results show that the project is economically attractive, and it can be carried out with standard matured solutions of hydro turbines available at the market

Risks in the Role of Co-Creating the Future of Tourism in “Stigmatized” Destinations

The primary goal of this paper was to investigate the strength of the influence of different types of risk on the travelers’ intention to visit destinations in future, that are, prejudiced due to COVID-19, marked as “stigmatized”, “isolated”, or “risky destinations”. Field interview research was conducted at the “Belgrade Nikola Tesla Airport” (Serbia). The results obtained by multiple regression analysis showed that all types of risks influenced the intention of travelers, with financial risk showing a more significant impact. Canonical discriminant analysis indicated that men were most afraid of human induced risk, service quality risk, natural disaster and COVID-19 risk, and they chose safer destinations. Among the women, the biggest fear was financial risk, socio-psychological risk, and food safety risk. Older respondents and those under the influence of external factors decided on safer destinations, while financial status did not play a significant role in predicting the choice of destination. The selection of the destination according to the degree of security was determined by the ordinal regression methodology. The entire research presents a certain novelty, because so far in the numerous studies on the topic of the negative consequences of COVID-19 on tourism, there has been no discussion of stigmatized or risky destinations that received that epithet, and were therefore negatively and unfairly marked in the minds of tourists for future visits