9 research outputs found
Modulation of topoisomerase IIα expression and chemosensitivity through targeted inhibition of NF-Y:DNA binding by a diamino p-anisyl-benzimidazole (Hx) polyamide
BACKGROUND: Sequence specific polyamide HxIP 1, targeted to the inverted CCAAT Box 2 (ICB2) on the topoisomerase IIα (topo IIα) promoter can inhibit NF-Y binding, re-induce gene expression and increase sensitivity to etoposide. To enhance biological activity, diamino-containing derivatives (HxI*P 2 and HxIP* 3) were synthesised incorporating an alkyl amino group at the N1-heterocyclic position of the imidazole/pyrrole. METHODS: DNase I footprinting was used to evaluate DNA binding of the diamino Hx-polyamides, and their ability to disrupt the NF-Y:ICB2 interaction assessed using EMSAs. Topo IIα mRNA (RT-PCR) and protein (Immunoblotting) levels were measured following 18h polyamide treatment of confluent A549 cells. γH2AX was used as a marker for etoposide-induced DNA damage after pre-treatment with HxIP* 3 and cell viability was measured using Cell-Titer Glo®. RESULTS: Introduction of the N1-alkyl amino group reduced selectivity for the target sequence 5'-TACGAT-3' on the topo IIα promoter, but increased DNA binding affinity. Confocal microscopy revealed both fluorescent diamino polyamides localised in the nucleus, yet HxI*P 2 was unable to disrupt the NF-Y:ICB2 interaction and showed no effect against the downregulation of topo IIα. In contrast, inhibition of NF-Y binding by HxIP* 3 stimulated dose-dependent (0.1-2μM) re-induction of topo IIα and potentiated cytotoxicity of topo II poisons by enhancing DNA damage. CONCLUSIONS: Polyamide functionalisation at the N1-position offers a design strategy to improve drug-like properties. Dicationic HxIP* 3 increased topo IIα expression and chemosensitivity to topo II-targeting agents. GENERAL SIGNIFICANCE: Pharmacological modulation of topo IIα expression has the potential to enhance cellular sensitivity to clinically-used anticancer therapeutics. This article is part of a Special Issue entitled: Nuclear Factor Y in Development and Disease, edited by Prof. Roberto Mantovani
Charge-Transfer Intermediates in the Electrochemical Doping Mechanism of Conjugated Polymers
: We address the nature of electrochemically induced charged states in conjugated polymers, their evolution as a function of electrochemical potential, and their coupling to their local environment by means of transient absorption and Raman spectroscopies synergistically performed in situ throughout the electrochemical doping process. In particular, we investigate the fundamental mechanism of electrochemical doping in an oligoether-functionalized 3,4-propylenedioxythiophene (ProDOT) copolymer. The changes embedded in both linear and transient absorption features allow us to identify a precursor electronic state with charge-transfer (CT) character that precedes polaron formation and bulk electronic conductivity. This state is shown to contribute to the ultrafast quenching of both neutral molecular excitations and polarons. Raman spectra relate the electronic transition of this precursor state predominantly to the Cβ-Cβ stretching mode of the thiophene heterocycle. We characterize the coupling of the CT-like state with primary excitons and electrochemically induced charge-separated states, providing insight into the energetic landscape of a heterogeneous polymer-electrolyte system and demonstrating how such coupling depends on environmental parameters, such as polymer structure, electrolyte composition, and environmental polarity
Charge-Transfer Intermediates in the Electrochemical Doping Mechanism of Conjugated Polymers
In this work, we address the nature of electrochemically induced charged states in conjugated polymers, their evolution as a function of electrochemical potential, and their coupling to their local environment by means of transient absorption and Raman spectroscopies synergistically performed in situ throughout the electrochemical doping process. In particular, we investigate the fundamental mechanism of electrochemical doping in an oligoether-functionalized 3,4-propylenedioxythiophene (ProDOT) copolymer. The changes embedded in both linear and transient absorption features allow us to identify a precursor electronic state with charge-transfer (CT) character that precedes polaron formation and bulk electronic conductivity. This state is shown to contribute to the ultrafast quenching of both neutral molecular excitations and polarons. Raman spectra relate the electronic transition of this precursor state predominantly to the Cβ -Cβ stretching mode of the thiophene heterocycle. We characterize the coupling of the CT-like state with primary excitons and electrochemically induced charge separated states, providing insight into the energetic landscape of a heterogeneous polymer-electrolyte system and demonstrate how such coupling depends on environmental parameters, such as polymer structure, electrolyte composition, and environmental polarity.<br /
Effects of Side-Chain Length and Functionality on Polar Poly(dioxythiophene)s for Saline-Based Organic Electrochemical Transistors.
Understanding the impact of side chains on the aqueous redox properties of conjugated polymers is crucial to unlocking their potential in bioelectrochemical devices, such as organic electrochemical transistors (OECTs). Here, we report a series of polar propylenedioxythiophene-based copolymers functionalized with glyme side chains of varying lengths as well as an analogue with short hydroxyl side chains. We show that long polar side chains are not required for achieving high volumetric capacitance (C*), as short hydroxy substituents can afford facile doping and high C* in saline-based electrolytes. Furthermore, we demonstrate that varying the length of the polar glyme chains leads to subtle changes in material properties. Increasing the length of glyme side chain is generally associated with an enhancement in OECT performance, doping kinetics, and stability, with the polymer bearing the longest side chains exhibiting the highest performance ([μC*]OECT = 200 ± 8 F cm-1 V-1 s-1). The origin of this performance enhancement is investigated in different device configurations using in situ techniques (e.g., time-resolved spectroelectrochemistry and chronoamperometry). These studies suggest that the performance improvement is not due to significant changes in C* but rather due to variations in the inferred mobility. Through a thorough comparison of two different architectures, we demonstrate that device geometry can obfuscate the benchmarking of OECT active channel materials, likely due to contact resistance effects. By complementing all electrochemical and spectroscopic experiments with in situ measurements performed within a planar OECT device configuration, this work seeks to unambiguously assign material design principles to fine-tune the properties of poly(dioxythiophene)s relevant for application in OECTs