Sexual dysfunction genital reasons among women

We have studied sexual discomfort among women in the age from 20 to 60 (n=159). Average age is 44,5+8,1 years. 123 patients (77,3%)noted various types of sexual discomfort, in 50-80% cases characterized by dryness and burning pain at vaginal orifice. Women with regular sexual relations having orgasm (p=0,03) feel discomfort less often. To eliminate discomfort 41,5% of women on their own used lubricants. 81,8% from them noted absence of discomfort and presence of orgasm, 50,0% of women not using lubricants noted discomfort and absence of orgasm. Conclusions: one of the reasons causing absence of women’s orgasm is discomfort during sex, use of lubricants during sex increase women's capability to orgasm.Изучены проявления сексуального дискомфорта у женщин в возрасте от 20 до 60 лет (п=159). Средний возраст 44,5+8,1 лет. 123 пациентки (77,3%) отметили различные проявления сексуального дискомфорта, в 50-80% случаев характеризующиеся сухостью и жжением во входе во влагалище. Достоверно реже дискомфорт испытывали женщины, ведущие регулярную половую жизнь и испытывавшие при этом оргазм (р=0,03). Для устранения дискомфорта 41,5% женщин самостоятельно использовали лубриканты. 81,8% из них отметили отсутствие дискомфорта и наличие оргазма, 50,0% женщин, не использовавших лубриканты, отмечали выраженный дискомфорт и отсутствие оргазма. Выводы: одной из причин отсутствия оргазма у женщины является наличие дискомфорта во время сексуальной близости, использование лубриканта во время полового акта повышает способность женщины к оргазму

Vaginal lubricants as vagina mucous trophic and secret function correction factor in childbearing and menopausal age

We have assessed the amount of vaginal leucorrhoea, pH of vaginal environment and vagina epithelium state among women of childbearing age (31,3+4,2 years old) -1 stgroup, and in menopausal transfer age (51,5+2,2 years old) - 2nd group, using lubricant containing D-panthenol at sexual contacts. Among women in childbearing age as wellas in menopausal transfer age it is notedthat trophic and secret vagina epithelium functions uffers resulting in thinning of epithelium, development of atrophic processes and decrease of vagina liquid production. Half of women in childbearing age and almost all women in menopausal transfer age has unbalanced vaginal acid-base environment. Use of lubricants containing D- panthenol improves vaginal epithelium trophic and secret function and does not affect vaginal acid-base environment, what supposes corresponding drug correction and development of lubricants maintaining vagina acid reaction.У женщин репродуктивного возраста (31,3+4,2 лет) -1 группа и находящихся в периоде менопаузального перехода (51,5+2,2 пет) - 2 группа, использующих во время сексуальных контактов пубрикант, содержащий Д-пантенол, проводили оценку количества вагинальных белей, pH влагалищной среды, состояния эпителия впагалища. Установлено, что женщин и в репродуктивный, и в возрасте менопаузального перехода страдает трофическая и секреторная функция эпитепия влагалища, проявляющаяся в истончении эпителия, развитии атрофических процессов и снижении продукции влагалищной жидкости. Кислотно-щелочное состояние впагалища нарушено у половины женщин репродуктивного возраста и у подавляющего числа женщин в возрасте менопаузального перехода. Применение пубрикантов, содержащих Д-пантенол, позволяет улучшить трофическую и секреторную функцию эпителия влагалища, однако не влияет на кислотно-щелочное состояние вагинальной среды, что предполагает соответствующую медикаментозную коррекцию, а также разработку пубрикантов, способствующих поддержанию кислой реакции влагалища

Başkortostan Cumhuriyeti Toratau Jeoparki bal arısı (apis mellifera) popülasyonunda tergit rengi değişimi

A phenetic analysis of the honey bee population of the Toratau Geopark (Russia) was performed. Over 1,000 worker and drone bee samples were collected from 250 colonies in 59 apiaries on the territory of the Toratau Geopark (Gafuriysky, Ishimbaysky, Meleuzovsky, and Sterlitamaksky districts of the Republic of Bashkortostan). Six phenes in worker bees and four phenes in drone bees were recognized. The phenes E, 1R, 2R, and 3R in workers and Is, I, and O-gray in drones were predominant in the honey bee population of the Toratau Geopark, which were associated with subspecies of the Clineage. These phenes can be used as indicators of introgressive hybridization in the local dark European honey bee population. The phenes allow for quick evaluation of certain honey bee colonies hybridization states.Toratau Jeoparkı'ndaki (Rusya) bal arısı popülasyonunun fenetik analizi yapılmıştır. Toratau Jeoparkı topraklarındaki (Başkurdistan Cumhuriyeti'nin Gafuriysky, Ishimbaysky, Meleuzovsky ve Sterlitamaksky bölgeleri) 59 arılıktaki 250 koloniden 1.000'den fazla işçi ve erkek arı örneği toplanmıştır. İşçi arılarda altı fen ve erkek arılarda dört tergit rengi tespit edilmiştir. İşçilerde E, 1R, 2R ve 3R ve erkek arılarda Is, I ve O-gri fenleri Toratau Jeoparkı'ndaki bal arısı popülasyonunda baskındı ve bunlar C soyunun alt türleriyle ilişkiliydi. Bu tergit rengi, yerel koyu Avrupa bal arısı popülasyonunda içsel melezleşmenin göstergeleri olarak kullanılabilir. Bu tergit renkleri, belirli bal arısı kolonilerinin melezleşme durumlarının hızlı bir şekilde değerlendirilmesine olanak sağlamaktadır.The article was prepared with supporting the grant of the head of the Republic of Bashkortostan Radiy Khabirov, grant title "Study of the Bashkir bee population on the territory of the Toratau Geopark

Monitoring of coronavirus infection in the kyrgyz population

Purpose of the study: to study the dynamics of developing herd immunity against SARS-CoV-2 in the population of the Republic of Kyrgyzstan during COVID-19. Materials and methods. The work was carried out using the methodology for assessing population immunity developed by Rospotrebnadzor (Russia) as well as the Ministry of Health (Kypgyzstan) and the St. Petersburg Pasteur Institute. The selection of participants was carried out by questionnaire using a cloud (Internet server) service. To monitor population immunity, a cohort of 2421 subjects was formed, who participated in all stages of seromonitoring. Volunteers were randomized according to age groups (1–17, 18–29, 30–39, 40–49, 50–59, 60–69, 70+ years), regional and professional factors. Antibodies (Abs) against SARS-CoV-2 nucleocapsid (Nc) and the receptor binding domain (RBD) of S-glycoprotein were determined by qualitative and quantitative methods. The study was carried out in 3 stages according to a single scheme: 1st stage — 06/28–07/03/2021, 2nd — 21–25/02/2022 and 3rd — 31/10–04/11/2022. Since 2021, Kyrgyzstan has been vaccinating the population against SARS-CoV-2 mainly using inactivated whole-virion vaccines. Results. Population immunity against SARS-CoV-2 was predominantly accounted for by both Ab types (Nc+RBD+). By the 3rd stage, the percentage of such persons reached 99.2%, Nc–RBD– volunteers — up to 0.8%. At the 1st stage, middle-aged people dominated, but age differences were leveled out by the 2nd stage. The greatest impact on seroprevalence was found among medical workers, the smallest — among businessmen and industrial workers. Populational vaccination significantly impacted on the state of herd immunity that reached 25% by the 3rd stage. The refusals of the population in Kyrgyz Republic from vaccination noted at the 2nd and especially 3rd stages did not significantly affect level of herd immunity, which could probably be associated with asymptomatic cases of COVID-19, against which primary vaccination had a booster effect. Conclusion. The dynamics of population humoral immunity against SARS-CoV-2 included a number of changes in the level of circulating antibodies (Nc, RBD), caused by both primary infection and vaccination. The herd immunity formed in population of Kyrgyzstan allowed to reduce the incidence of COVID-19 to almost sporadic level

Evaluating protein cross-linking as a therapeutic strategy to stabilize SOD1 variants in a mouse model of familial ALS

Mutations in the gene encoding Cu-Zn superoxide dismutase 1 (SOD1) cause a subset of familial amyotrophic lateral sclerosis (fALS) cases. A shared effect of these mutations is that SOD1, which is normally a stable dimer, dissociates into toxic monomers that seed toxic aggregates. Considerable research effort has been devoted to developing compounds that stabilize the dimer of fALS SOD1 variants, but unfortunately, this has not yet resulted in a treatment. We hypothesized that cyclic thiosulfinate cross-linkers, which selectively target a rare, 2 cysteine-containing motif, can stabilize fALS-causing SOD1 variants in vivo. We created a library of chemically diverse cyclic thiosulfinates and determined structure-cross-linking-activity relationships. A pre-lead compound, “S-XL6,” was selected based upon its cross-linking rate and drug-like properties. Co-crystallographic structure clearly establishes the binding of S-XL6 at Cys 111 bridging the monomers and stabilizing the SOD1 dimer. Biophysical studies reveal that the degree of stabilization afforded by S-XL6 (up to 24°C) is unprecedented for fALS, and to our knowledge, for any protein target of any kinetic stabilizer. Gene silencing and protein degrading therapeutic approaches require careful dose titration to balance the benefit of diminished fALS SOD1 expression with the toxic loss-of-enzymatic function. We show that S-XL6 does not share this liability because it rescues the activity of fALS SOD1 variants. No pharmacological agent has been proven to bind to SOD1 in vivo. Here, using a fALS mouse model, we demonstrate oral bioavailability; rapid engagement of SOD1G93A by S-XL6 that increases SOD1G93A’s in vivo half-life; and that S-XL6 crosses the blood–brain barrier. S-XL6 demonstrated a degree of selectivity by avoiding off-target binding to plasma proteins. Taken together, our results indicate that cyclic thiosulfinate-mediated SOD1 stabilization should receive further attention as a potential therapeutic approach for fALS

Chemical composition of bottom sediments from the Deryugin Basin, Sea of Okhotsk

This paper reports data including new analyses of contents of Ni, Co, V, Mo, Fe, Mn, Zn, Ba, Sc, Y, Cd, Rb, Cs, and W in bottom sediments of the Deryugin Basin. Features of chemical element distribution in the bottom area were identified and zones of maximum accumulation of major and trace elements were allocated. A correlation between the elements was shown