PENGOPTIMALAN METODE KROMATOGRAFI CAIR KINERJA TINGGI DALAM ANALISIS SENYAWA DELTAMETHRIN SEBAGAI RESIDU DALAM PRODUK ASAL HEWAN

Penelitian ini bertujuan mendapatkan prosedur atau metode kromatografi cair kinerja tinggi (KCKT) yang valid dan optimal dalam analisis deltamethrin sebagai senyawa yang berpotensi menjadi residu dalam produk hewan. Alat utama yang digunakan dalam penelitian ini adalah satu set KCKT Shimadzu 6.1, dengan kolom C-18 (30° C), panjang gelombang detektor UV-vis 236 nm. Fase gerak yang digunakan dalam penelitian ini adalah asetonitril 80% dalam akuabides yang dialirkan dengan laju 1,25 ml/menit. Hasil penelitian menghasilkan kromatogram yang terlihat menunjukkan peak area yang nyata terpisah dari senyawa lain. Batas deteksi diketahui pada konsentrasi 0,1 μg/ml, sedangkan batas kuantifikasi pada konsentrasi 0,5 μg/ml. Rerata luas area untuk konsentrasi 0,5; 1; 1,5; 2; 5; dan 10 μg/ml masing-masing adalah 18.255,33; 47.142,00; 55.587,00; 64.181,33; 204.269,00; dan 395.918,00 dengan persamaan garis linier y= 39.866x-1.719,5 (R= 0,99). Hasil analisis juga menunjukkan presisi dan akurasi hasil yang baik. Hasil penelitian dapat disimpulkan bahwa metode yang dikembangkan pada penelitian ini mempunyai validitas yang baik dan optimal untuk analisis deltamethrin, yang merupakan senyawa potensial menjadi residu pada produk asal hewan

PROSEDUR ANALISIS VALIDASI DELTAMETHRIN PADA PAKAN AYAM MENGGUNAKAN ALAT KROMATOGRAFI CAIR KINERJA TINGGI Penggunaan Kolom C 18, Fase Gerak Asetonitril 80% dalam Akuabides, Laju Alir Fase Gerak 1,25 ml/menit, Panjang Gelombang 236 nm dan Suhu 30 0 C

Currently the use of.HPLC have been widely used to determine pesticides residues due to the simple process with fast analysis time. Food safety factor becomes important as the increasing of consumer awareness to their food quality. The quality of food resources is not only measured from its high nutrient but also free from pesticide. The aim of this study is to validate the deltamethrin analysis procedure on chicken feed by using high-performance liquid chromatography. The deltamethrine concentration used in this research were 1ug/ml, 2 ug/ml, and 5 ug/ml. The main tool used in this study was HPLC Shimadzu 6.1 with a mobile phase 80% acetonitrile in distilled water, mobile phase flow rate of 1.25 ml / min, and a temperature of 30oC. In addition, C 18 column with UV-Vis detector wavelength of 236 nm was used here. Validation parameters used in this study were the specificity, precision, accuracy, detection limit, quantification limit, and linearity. The results showed deltamethrin peak area in HPLC analysis specifically appears within 8 to 9.5 minutes. The mean area of each concentration of 1ug/ml, 2ug/ml and 5ug/ml were respectively 58515.67, 87792.67 and 233404.67. Specificity of each concentration showed good results because the peak area of deltamethrin were undisturbed by other substances. The precision value have a good value due to the results of RSD � 2%. The accuracy value of each concentration fulfill the standards value that are within 80-120%. The detection limit of the instrument has a value of 0.28 ug/ml and for the non instrument does not exist because the area of standard sample can be read. The quantification limit of the instrument has a value of 0.96 ug/ml and for the non-instrument is 0.5 ug/ml. As for the linearity gives an equation y = 44814x + 7100 with a value of R = 0.99. It can be concluded that the procedures used in this study have good validity

ANALISIS KESESUAIAN UJI KAPPA DENGAN MEMBANDINGKAN DUA METODE ANALISIS DELTAMETHRIN MENGGUNAKAN ALAT KROMATOGRAFI CAIR KINERJA TINGGI

Development of analysis using chromatography was shown by the appearance of High Performance Liquid Chromatography (HPLC) which can classified the organic chemical compound that didn�t vaporize with speed and high efficiency. Several of delthamethrin analysis method has been conducted in Pharmacology Department of Veterinary Medicine Faculty of Gadjah Mada University. This research intent on comparing and choosing delthamethrin analysis method using HPLC which has been developed by Pharmacology Laboratory of Veterinary Medicine Faculty of Gadjah Mada University and performing agreement analysis to the selected method by using kappa test. The instrument used as the analyzer in this research was a set of HPLC created by Shimadzu 6.1, SCL-10A VP system controled, the moving phase acetonitril Merck 80% in a sterile aquabidest, LC-10AD vP pump, collumn C18 Shim-Pack VP-ODS (150 x 4,6 mm) with temperature 300 C, injector, CTO-10C vP oven, UV-Vis SPD-10AV detector, DGU-14A degasser, and LC-10AV vP liquid chromatography. The material that used to the agreement analysis for the selected method is forty sampels of liver solution extract. The comparison result between method I and method II shows that method II gives a better result in analyzing deltamethrin. The agreement analysis result with kappa test shows a good agreement result (agreement value = 0,7)

HUBUNGAN PERLAKUAN DELTAMETRIN PADA AIR MINUM BERBAGAI KONSENTRASI TERHADAP KANDUNGAN RESIDU DELTAMETRIN PADA DARAH, HATI, DAN DAGING AYAM BROILER

Pesticide is an important material in agriculture or livestock sector, especially in eradicating insects. Non procedural pesticide usage can lead to issues such as the presence of residue in agriculture/livestock products. This study aimed to determine the effect of various concentrations of deltametrin treatment in drinking water on the residue content in the blood, liver, and meat of broilers. Nine broilers were divided into three groups. Group 1 was the control, Group 2 was group given deltametrin 5 mg/L in drinking water, and Group 3 was group given deltametrin 10 mg/L in drinking water. Broilers were kept in Education and Training Unit of Animal Health (UP2KH) cage for 35 days and residue inspection in blood, liver, and meat was conducted after the harvest. Deltametrin residue analysis was performed at the Laboratory of Pharmacology of FKH UGM. Analysis of correlation showed that there was a significant linear relationship between various deltametrin concentration and residue concentration in meat (r>0,

VALIDASI PROSEDUR ANALISIS DELTAMETHRIN PADA DAGING AYAM BROILER MENGGUNAKAN ALAT KROMATOGRAFI CAIR KINERJA TINGGI (KCKT)

Analysis technology of pesticide residues more develop in a row with increases usage pesticide in a field. There are several of residues analysis method, one of them is HPLC. This research aims to perform validate posseses of analysis deltamethrin using a Shimadzu 6.1 high-performance liquid chromatography (HPLC) with control system SCL-10 A VP, DGU-14 A degasser. Mobile phase of Merck acetonitrile 80% in steryl aquadest, flow rate of 1.25 ml/minute, UV-Vis SPD 10 AV VP detector with wavelength of 236 nm and used Shimadzu Shim-pack 150 L x 4.6 mm VP-ODS Column C18 on temperature 30 oC. The validation examination is based on specificity, precision, accuracy, limit of qualitative, quantitative limits, and linearity. Results of the research shows the average of area concentration 1 μg/ml, 2 μg/ml, and 5 μg/ml in consecutive are 34620.33, 79048.67, dan 181208.7 with retention time in 8.6-8.98 minute. Repeatability is clone 3 times and the RSD mean value obtained is � 2%. The detection limit at 0.1 μg/ml and limit of quantification at concententration 1 μg/ml. The equation of linear line y = 36046x+2182, with the value of R= 0.99. It can be concluded that the method used in the research posseses good validity

VALIDASI PROSEDUR ANALISIS DELTAMETHRIN PADA HATI AYAM MENGGUNAKAN ALAT KROMATOGRAFI CAIR KINERJA TINGGI (KCKT)

Deltamethrin as a pesticide widely used in agriculture and estate sector. Residue of pesticide in food derivate from animals (included offal) has been reported in Indonesia and it would be harmful for health if it is exceeded a predetermined threshold. It caused analysis pesticide need to do. High Performance Liquid Chromatography (HPLC) is one of developing technology analysis pesticide�s residue. High performance liquid chromatography instrument adequate of analyze a variety of shots both qualitatively and quantitatively, either single or mixture component of pesticide. An object of this study is validate the methods of analysis deltamethrin using HPLC instrument based on the specificity, precision, accuracy, limit of detection, limit of quantification, and linearity. The main material used is set HPLC, Shimadzu 6.1, deltamethrin, and chicken�s liver. The support materials is acetonitrile, distilled water, test tubes, spuid, parafilm, vortex mixer, ultrasonic bath, shaker, timers, scales, micropipette, and HPLC injector. Methods of study with smooth liver tissue was then added deltamethrin at a concentration of 1 mg 1 μg/ml, 2 μg/ml, dan 5 μg/ml in a test tube. Cap test tube close with parafilm, and then each in a vortex for 1 minute, put in an ultrasonic bath for 15 minute at 40 0C, then homogenization in shaker with a speed of 150-200 beat/minute during 20 minutes. Then all the tubes centrifuged during 12 minutes at speed 13,000 rpm. The supernatant was taken and injection to HPLC. Results showed the mean cup of deltamethrin separated from other compounds in the retention time between 8 to 9.5 minutes at a concentration of 1 μg/ml, 2 μg/ml, and 5 μg/ml in the test specificity. Indicated precision for each area concentration in a series are 43.01

KAJIAN FAKTOR�FAKTOR YANG DIDUGA BERASOSIASI DENGAN RESIDU DELTAMETHRIN PADA AYAM BROILER DI KECAMATAN BERBAH DAN KALASAN KABUPATEN SLEMAN

Broiler is a type of chicken that is highly effective in meat production. Well planned farm management can ensure that the maximum meat production ofbroilers. The chicken farm system that is commonly used is litter system. Pest control is an important aspect of animal health management and biosecurity on farms. The use of pesticides is one of the methods in pest controlling. As Indonesia is mainly depending on the farming sector, pesticides are frequently used in this country. The overuse of pesticides can pollute the environment. Deltamethrin as a broad-spectrum pyrethroid, is the currently most widely used insecticide in Indonesia because of its lethal effect on insects. The overuse of chemical-based pesticides can be left in the cycle and can be either directly or indirectly consumed by humans. This thesis aimed to understand the factors associated with exposure ofdeltamethrin residual in broilers atBerbahand Kalasan districts, Sleman. Samples of blood, liver, and muscle from the left drumstick were taken from chickens from 15 farms in Berbah and Kalasan districts, Sleman.Detecting of pesticide residue was conducted at the Pharmacology Laboratory, Faculty of Veterinary Medicine, Gadjah Mada University. Interviews and questionnaires were conducted to gather information on farming management practices. Data analysis by using Chi-square test and relative risk analysis was to determine management processes which had a significant association. The result of chi-square showed that there wassignificant relationship between exposure of deltamethirn in broilers with several management factors (P<0,1). The relative risk analysis found that the following management practices were associated with deltamethrin residue: business system (RR= 2,585), the responsibilities within farm policies (RR= 0,302), waste handling (RR= 0,396), cage type (RR= 0,579), feed storage (RR= 1,708), water channels (RR= 2,434), and harvest period (RR= 1,893). Advice given was to conduct research with a wider scope to find out the recommendations of a more comprehensive broiler management especially in reducing the risk of exposure to deltamethrin

PENGARUH PEMAPARAN DELTAMETHRIN TERHADAP BERAT BADAN DAN FEED CONVERSION RATIO PADA AYAM BROILER

Deltamethrin is a type-II pyrethroid insecticide that is widely used in Indonesia. This insecticide is effective against insects via direact contact and ingestion. The aim of this research was to determine the effects of deltamethrin on the body weight and Feed Conversion Ratio of broiler. The total number of ten broilers was divided into two groups of five. Group I as a control group, were given standard feed and water. Group II as the experimental group were given standard feed and water containing 20 mg/L of deltamethrin. The feed consumption was weighed daily until the 35th day while the body weight of chickens was weighed weekly until the 5th week. FCR was calculated by dividing the total feed consumption with the body weight gain. The body weight gain of each group was analyzed by using Friedman test. Mann- Whitney test was used in analyzing the difference in body weight gain and FCR between both groups. The result showed that the body weight of the control group increased significantly from 119 ± 2,12g in the first week to 2546 ± 93,70g in the fifth week (P<0.05). The body weight of experimental group increased significantly from 112.4 ± 4.51g in the first week to 2036 ± 271.07g in the fifth week (P<0.05). The mean of Feed Conversion Ratio of the control group was 1,69 ± 0,07g, while for the experimental group was 1,95 ± 0,23g. The result of Mann-Whitney test showed that there was no significant difference between both groups. Based on the result, it can be concluded that deltamethrin has no effect on Feed Conversion Ratio, however, can reduce the weight gain of broilers

VALIDASI ANALISIS METODE DELTAMETHRIN MENGGUNAKAN ALAT KROMATOGRAFI CAIR KINERJA TINGGI (KCKT) : Penggunaan Kolom C18, Fase Gerak Asetonitril 80% dalam Akuabides, Laju Alir Fase Gerak 1,25 ml/menit, dan Panjang Gelombang Detektor UV-Vis 236 nm

Pesticide residues contained in food from livestock may harm human�s health if it exceeds the predetermined threshold, therefore we need to develop analysis research of pesticide residues. One of the pesticide residues commonly used in food from livestock is deltamethrin. This research was aimed at validating the method analysis of deltamethrin using High Performance Liquid Chromatography (HPLC) based on specificity, precision, limit of detection, limit of quantification, and linearity. The main instrument used in this research was a set of High Performance Liquid Chromatography (HPLC) with brand of Shimadzu version 6.1 which is the property of Pharmacology Laboratory, Faculty of Veterinary Medicine of UGM with the colomn used was C18, wavelength of 236 nm and the flow of 1.25 ml/min. Phases of mobile used in this research was 80% acetonitrile in aquabidest. The results showed the average of peak area for each concentration 0,5 µg/ml, 1 µg/ml, 2 µg/ml, 5 µg/ml, and 10 µg/ml, were 18255.33, 47142, 64181.33, 204269, and 395918, with a linear equation of y = 39819x-1146,1 and R = 0,998. Limit of detection was at concentration 0,1 µg/ml, and limit of quantification was at concentration of 0.5 µg/ml. Chromatogram showed the peak area which is obviously separated from other substances. It can be concluded that the method used has good validity as seen from spesificity, precision, LOD, LOQ, and linearity