Examples of positive and negative staining for THSD7A, PLA2R, and IgG4.

<p>Positive staining for THSD7A (a, b), PLA2R (e, f), and IgG4 (i, j), and negative staining for THSD7A (c, d), PLA2R (g, h), and IgG4 (k, l) are shown.</p

Prevalence of enhanced granular expression of thrombospondin type-1 domain-containing 7A (THSD7A) and M-type phospholipase A2 receptor (PLA2R) in the glomeruli of patients with idiopathic membranous nephropathy (MN) and secondary MN.

<p>Prevalence of enhanced granular expression of thrombospondin type-1 domain-containing 7A (THSD7A) and M-type phospholipase A2 receptor (PLA2R) in the glomeruli of patients with idiopathic membranous nephropathy (MN) and secondary MN.</p

Additional file 4 of Pure somatic pathogenic variation profiles for patients with serrated polyposis syndrome: a case series

Additional file 4: Table S4. Mutational signature of the single base substitution

Characteristics of idiopathic membranous nephropathy (MN) patients with enhanced granular expression of thrombospondin type-1 domain-containing 7A (THSD7A) or M-type phospholipase A2 receptor (PLA2R) in the glomeruli.

<p>Characteristics of idiopathic membranous nephropathy (MN) patients with enhanced granular expression of thrombospondin type-1 domain-containing 7A (THSD7A) or M-type phospholipase A2 receptor (PLA2R) in the glomeruli.</p

Immunohistochemical analysis for thrombospondin type-1 domain-containing 7A (THSD7A) and M-type phospholipase A2 receptor (PLA2R).

<p>Immunostaining for THSD7A (a, c, e) and PLA2R (b, d, f) in patients with idiopathic membranous nephropathy (a–d) and secondary membranous nephropathy (e, f). Original magnification: ×400. Inset, enhanced granular expression of THSD7A (a) and PLA2R (d) shown at a higher magnification (arrows). Abbreviations: THSD7A, Thrombospondin type-1 domain-containing 7A; PLA2R, M-type phospholipase A2 receptor; MN, membranous nephropathy.</p

Additional file 1 of Pure somatic pathogenic variation profiles for patients with serrated polyposis syndrome: a case series

Additional file 1: Table S1. DNA variant profile (Case #1)

Additional file 1 of Using a multistep approach with multidisciplinary team to increase the diagnosis rate of Lynch syndrome-associated colorectal cancer after universal screening: a single-center study in Japan

Supplementary Material

Baseline characteristics of the AAA group and control group.

<p>The definition of each item: hypertension (medication for hypertension or a systolic blood pressure ≧140 mmHg and/or a diastolic blood pressure ≧90 mmHg), hypercholesterolemia (medication for hypercholesterolemia or total cholesterol concentration in the serum of ≧220 mg/dL), hypertriglyceridemia (medication for hypertriglyceridemia or total triglyceride in the serum of ≧150 mg/dL), diabetes (present or past medication for diabetes), smoking (present or past smoking history).</p

Macrophage infiltration of AAA microvessels.

<p><b>A</b>, Elastica van Gieson staining of abdominal aortic aneurysm (AAA). Immunohistochemistry for podoplanin (<b>B</b>) and macrophages (<b>C</b>), CD19 (<b>E</b>), and myeloperoxidase (MPO) of the AAA wall. <b>B</b>, Lymphatic microvessels in the intima/media of the AAA (red dotted line encircling lymphatic microvessels). <b>C</b>, Macrophages infiltration around/within lymphatic microvessels in the intima/media or in adventitia (red square: macrophages in intima/media, black square: macrophages in adventitia). <b>D</b>, CD3-positive T cells infiltration around/within lymphatic microvessels in the intima/media or in adventitia. <b>E</b>, CD19-positive B cells infiltration around/within lymphatic microvessels in the intima/media or in adventitia. <b>F</b>, MPO-positive neutrophils infiltration in the intima/media or in adventitia. <b>G–P</b>, Double immunofluorescence staining of macrophages infiltrating the intima/media (In) and adventitia (Ad). <b>G–P</b>, Macrophage: green, CD11b (<b>G</b>)/LYVE-1 (<b>H</b>)/VEGF-C (<b>I</b>)/MMP-9 (<b>J</b>)/TGF-β1 (<b>K</b>)/IL-4 (<b>L</b>)/IL-8 (<b>M</b>)/MIP-1α (<b>N</b>)/IFN-γ (<b>O</b>)/MCP-1 (<b>P</b>): red, DAPI: blue. LYVE-1, VEGF-C, MMP-9, TGF-β1, IL-4, IL-8, MIP-1α, and MCP-1 were expressed in the CD11b-positive macrophages in the intima/media, but not by macrophages in adventitia. <b>Q</b>, Double immunofluorescence staining of T-cells in intima/media and inflammatory cytokines. CD3: green, VEGF-C/MMP-9/TGF-β1/IL-4/IL-8/MIP-1α/IFN-γ/MCP-1: red, DAPI: blue. TGF-β1, IL-4, and IFN-γ were expressed in CD3-positive T lymphocytes in the intima/media. <b>R</b>, Double immunofluorescence staining of B lymphocytes in the intima/media and inflammatory cytokines. CD19: green, VEGF-C/MMP-9/TGF-β1/IL-4/IL-8/MIP-1α/IFN-γ/MCP-1: red, DAPI: blue. These inflammatory cytokines were not expressed in CD19-positive B lymphocytes. <b>S</b>, Double immunofluorescence staining of neutrophils in intima/media and inflammatory cytokines. MPO: green, VEGF-C/MMP-9/TGF-β1/IL-4/IL-8/MIP-1α/IFN-γ/MCP-1: red, DAPI: blue. These inflammatory cytokines were not expressed in MPO-positive neutrophils. Scale bars indicated 100 µm (<b>A–F</b>) and 10 µm (<b>G–S</b>).</p

Fluorescence lymphography and microscopy of abdominal aortic aneurysm.

<p><b>A</b>, Three dimensional CT image of an infrarenal abdominal aortic aneurysm (AAA). <b>B</b>, Intraoperative photography of an infrarenal AAA. <b>C</b>, Intraoperative near-infrared fluorescence lymphography of indocyanine green (ICG) (red arrow: ICG fluorescence, yellow dotted-line: sample resection line). <b>D</b>, Macroscopic findings of the resected sample observed from the luminal side (yellow dotted line: cross-section line of the harvested sample). <b>E</b>, Near-infrared fluorescence lymphography of ICG in the resected aneurysmal wall observed from the luminal side (red arrow: ICG fluorescence, yellow dotted line: cross-section line of the harvested sample). <b>F</b>, Near-infrared fluorescence lymphography of ICG in the cross-sectioned AAA wall (red arrow: ICG fluorescence, up: luminal side, bottom: adventitial side). <b>G</b>, Fluorescence microscopy of ICG in the AAA wall (ICG: red, DAPI: blue). <b>H</b>, Serial frozen section with Elastica van Gieson staining. <b>I</b>, Higher magnification of the outlined area in <b>G</b>. <b>J</b>, Higher magnification of the outlined area in <b>H</b>. <b>K</b>, Immunohistochemistry for podoplanin in the outlined area in <b>G</b> and <b>H</b>. <b>L</b>, Immunohistochemistry to label macrophages in the outlined area in <b>G</b> and <b>H</b>. Scale bars indicate 50 µm (<b>G–L</b>).</p