18 research outputs found
Antibacterial Diterpenoid Against Pathogenic Oral Bacteria of Streptococcus Mutans ATCC 25175 Isolated From Sarang Semut (Myrmecodia Pendans)
Get PDFDiscovery of new antibacterial agent to treat oral disease caused by pathogenic oral bacteria is an attracted focus to more pay attention of researcher. The tropical natural product is the promising sources of new bioactive as antibacterial compounds. The epiphyte plant of Sarang Semut (M. pendans) empirically has been used as a drug to treat various diseases. Our previous research found that ethyl acetate extract of Sarang Semut inhibited the bacteria growth of Enterococcus faecalis. This study aims to isolate, structure determination and antibacterial activity evaluation of active compound against Streptococcus mutans ATCC 25175. The isolation by combination chromatography on normal and reverse phase resulted an antibacterial compound 1. Based on the analysis of the spectroscopic data including IR, FTIR, 1H-NMR, 13C-NMR, 2D-NMR as well as by mass spectroscopic (MS) and compare to reported data, structure of compound 1 was suggested as terpenoid type diterpene derivative. Antibacterial activity evaluation by Kirby-Bauer method of compound 1 against S. mutans ATCC 25175 showed inhibition zone values were 17.8, 14.5 and 11.1 mm at 10000, 5000 and 1000 ppm, respectively, while the MIC and MBC values were 18.125 ppm with MBC of 2500 ppm, respectively
THE N-HEXANE FRACTION OF MYRMECODIA PENDANS INHIBITS CELL SURVIVAL AND PROLIFERATION IN COLON CANCER CELL LINE
Get PDFObjective: Despite advanced treatment options available for colorectal cancer, many reported resistance and unresponsiveness to conventional chemotherapeutic agents. Therefore, it is urgent to discover a novel drug for colon cancer. Sarang Semut (Myrmecodia pendans), an Indonesian native plant, has been studied extensively due to its anti-cancer profiles. This study aimed to evaluate the anti-tumour activity of Sarang Semut in colon cancer cells.Methods: We evaluated cytotoxic activity of methanol extract as well as n-hexane and ethyl acetate fraction towards colon cancer cell lines (Caco-2 and HCT-116 cells) utilizing 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. The most potent fraction was evaluated further in inhibiting cell survival using MTT assay and cell proliferation using trypan blue exclusion assay as well as a clonogenic assay.Results: Our data showed that the n-hexane fraction of Sarang Semut induces more cell death than the methanol extract and ethyl acetate fraction. Therefore, we analyzed the n-hexane fraction further and found that the inhibitory concentration 50% (IC50) of the n-hexane fraction was 24 and 30 parts per million (ppm) for Caco-2 and HCT-116 cells, respectively. Moreover, it inhibited cell growth as well as cell colony formation, in particular, shown by the plating efficiency (P<0.05) and colony area per seed (P<0.01) of the control group were different to the treatment group.Conclusion: The n-hexane fraction of Sarang Semut demonstrates a high potential antitumor activity in colon cancer cell line
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