8 research outputs found
Hosszú időtartamú monokultúrás termesztésből és különböző vetésforgó rendszerekből származó növények arbuszkuláris mikorrhiza (AM) gomba-közösségeinek vizsgálata
Különböző időpontban történő mikorrhizaoltás és szárazság stressz hatása fűszerpaprika terméshozamára
Mikorrhiza oltóanyag hatása két fűszerpaprika termesztésére és a helyi arbuszkuláris mikorrhiza gombaközösségre
How arbuscular mycorrhizal fungi influence the defense system of sunflower during different abiotic stresses
The association between terrestrial plants and arbuscular mycorrhizal (AM) fungi is one of the most common and widespread mutualistic plant-fungi interaction. AM fungi are of beneficial effects on the water and nutrient uptake of plants and increase plant defense mechanisms to alleviate different stresses. The aim of this study was to determine the level of polyphenol oxidase (PPO), guaiacol peroxidase (POX) and glutathione S-transferase (GST) enzyme activities and to track the expression of glutathione S-transferase (GST) gene in plant-arbuscular mycorrhizal system under temperature- and mechanical stress conditions. Our results suggest that induced tolerance of mycorrhizal sunflower to high temperature may be attributed to the induction of GST, POX and PPO enzyme activities as well as to the elevated expression of GST. However, the degree of tolerance of the plant is significantly influenced by the age which is probably justified by the energy considerations
Expression patterns of cel5A–cel5B , two endoglucanase encoding genes of Thermobifida fusca
Expression patterns of
cel5A
and
cel5B
, two endoglucanase encoding genes of
Thermobifida fusca
were compared by quantitative real-time PCR. With Avicel as carbon source the transcript level of
cel5A
continuously increased until the 10th
hour of incubation and then a sharp decrease was observed, whereas
cel5B
presented a slow constitutive expression on this substrate. When the microcrystalline cellulose powder MN300 was used as the inducing carbon source, the expression patterns of the two genes were similar. A low initial level of expression was followed by a rapid increase at the 5th
hour of incubation; a transient repression was then observed at the 10th
hour but after this sampling time, the expression levels started to increase again. The relative expression levels of
cel5A
were always higher than those of
cel5B
. Differences in transcription patterns of these two genes can be explained with the imperfect structure of the CelR binding regulatory region of
cel5B