Unraveling the Role of Allo-Antibodies and Transplant Injury.

Alloimmunity driving rejection in the context of solid organ transplantation can be grossly divided into mechanisms predominantly driven by either T cell-mediated rejection (TCMR) and antibody-mediated rejection (ABMR), though the co-existence of both types of rejections can be seen in a variable number of sampled grafts. Acute TCMR can generally be well controlled by the establishment of effective immunosuppression (1, 2). Acute ABMR is a low frequency finding in the current era of blood group and HLA donor/recipient matching and the avoidance of engraftment in the context of high-titer, preformed donor-specific antibodies. However, chronic ABMR remains a major complication resulting in the untimely loss of transplanted organs (3-10). The close relationship between donor-specific antibodies and ABMR has been revealed by the highly sensitive detection of human leukocyte antigen (HLA) antibodies (7, 11-15). Injury to transplanted organs by activation of humoral immune reaction in the context of HLA identical transplants and the absence of donor specific antibodies (17-24), strongly suggest the participation of non-HLA (nHLA) antibodies in ABMR (25). In this review, we discuss the genesis of ABMR in the context of HLA and nHLA antibodies and summarize strategies for ABMR management

Computational Models for Transplant Biomarker Discovery.

Translational medicine offers a rich promise for improved diagnostics and drug discovery for biomedical research in the field of transplantation, where continued unmet diagnostic and therapeutic needs persist. Current advent of genomics and proteomics profiling called "omics" provides new resources to develop novel biomarkers for clinical routine. Establishing such a marker system heavily depends on appropriate applications of computational algorithms and software, which are basically based on mathematical theories and models. Understanding these theories would help to apply appropriate algorithms to ensure biomarker systems successful. Here, we review the key advances in theories and mathematical models relevant to transplant biomarker developments. Advantages and limitations inherent inside these models are discussed. The principles of key -computational approaches for selecting efficiently the best subset of biomarkers from high--dimensional omics data are highlighted. Prediction models are also introduced, and the integration of multi-microarray data is also discussed. Appreciating these key advances would help to accelerate the development of clinically reliable biomarker systems

Noninvasive Urinary Monitoring of Progression in IgA Nephropathy.

Standard methods for detecting and monitoring of IgA nephropathy (IgAN) have conventionally required kidney biopsies or suffer from poor sensitivity and specificity. The Kidney Injury Test (KIT) Assay of urinary biomarkers has previously been shown to distinguish between various kidney pathologies, including chronic kidney disease, nephrolithiasis, and transplant rejection. This validation study uses the KIT Assay to investigate the clinical utility of the non-invasive detection of IgAN and predicting the progression of renal damage over time. The study design benefits from longitudinally collected urine samples from an investigator-initiated, multicenter, prospective study, evaluating the efficacy of corticosteroids versus Rituximab for preventing progressive IgAN. A total of 131 urine samples were processed for this study; 64 urine samples were collected from 34 IgAN patients, and urine samples from 64 demographically matched healthy controls were also collected; multiple urinary biomarkers consisting of cell-free DNA, methylated cell-free DNA, DMAIMO, MAMIMO, total protein, clusterin, creatinine, and CXCL10 were measured by the microwell-based KIT Assay. An IgA risk score (KIT-IgA) was significantly higher in IgAN patients as compared to healthy control (87.76 vs. 14.03, p < 0.0001) and performed better than proteinuria in discriminating between the two groups. The KIT Assay biomarkers, measured on a spot random urine sample at study entry could distinguish patients likely to have progressive renal dysfunction a year later. These data support the pursuit of larger prospective studies to evaluate the predictive performance of the KIT-IgA score in both screening for non-invasive diagnosis of IgAN, and for predicting risk of progressive renal disease from IgA and utilizing the KIT score for potentially evaluating the efficacy of IgAN-targeted therapies

Review of Khatun,S.,2018, Australianama: The South Asian Odyssey in Australia,320pp, Hurst, London, ISBN: 9781849049696

This book deepens our understanding of South Asian migration. It is highly recommended for postgraduate students and researchers of the history of South Asian migration, migration narratives, Islam in Australia, Aboriginal history, and Aboriginal–South Asian storytelling. It could also be a reference in courses related but not limited to colonial and postcolonial histories, anthropology, feminism, and multiculturalism

Louise Lightfoot : in search of India

 Louise Lightfoot, a trained architect by profession and an ardent balletomane, is best known for moving away from pure Western classical Ballet to a fusion of classical technique and romantic emotion in Australia through her First Australian Ballet group and school. During late 1920s, she was impressed by the performances of Anna Pavlova and Uday Shankar and to bring more appropriateness and authenticity to her own Indian classical dance style that she was trying to experiment with, virtually unknown and unseen in Australia till then in its original form, Lightfoot took a few weeks stopover in India. This short holiday eventually stretched to months and then eight years as she travelled to Tamil Nadu and Kerala’s Kalamandalam, where she began her study of the complex traditions of Kathakali and Bharata Natyam dance. Here she also became a Stage Manager cum Artistic and Publicity director for local troupes and artistes in residence. She was so thrilled by the whole experience of learning Kathakali – involving poetry, song, acting and dance – that soon she started appealing to the British in India to not only appreciate the Indian dance but also to Indian parents to allow their sons and daughters to dance. Lightfoot, as Dance Director of Shivaram, Janaki Devi, Priyagopal Singh and Lakshman Singh, supported by an ensemble of Australian dancers including Ruth Bergner, Moya Beaver, Leona Welch, Pat Martin and Betty Russell, successfully toured and promoted a range of Indian classical dance forms, like Kathakali, Manipuri, Bharatanatyam, throughout Australia, New Zealand, and Fiji. As an early image-maker, she also paved the way for many other noted Indian dancers and troupes. In spite of decades of hard work and dedication to Indian dancing and creating awareness about India in Australia her work and life is little known! Her journey is fascinating because of the workings of race relations not just in Australia but also India – existing prejudices against “Whites.” In this paper I try to chart out through Australian and Indian newspaper reports her search for India

Melbourne’s Indian Film Festival needs to strengthen its ties

 The Indian Film Festival of Melbourne (IFFM) kicked off last night with Bollywood’s cult classic curry-western Sholay in 3D format. This year IFFM is screening 46 films from four countries in 17 languages, including Urdu, Nepalese, Himachli, Sinhala, and Sherdukpen. It’s the biggest film festival of its type in the southern hemisphere – but it’s attracted criticism from some in the Indian community in Melbourne for its failure to nurture ties between local filmmakers and the industry in India