A study on embryonic development of Yellow Fin Seabream (Acanthopagrus latus)

Embryonic and larval development stages of yellow fin seabream (Acanthopagrus latus) were studied in two average temperatures (21 and 24C) in Marine Fish Research Center in Emam Khomeini Port. Egg diameter in initiation of fertilization was 739.35 plus or minus 0.0081k and during final hatching it was 792.36 plus or minus 0.0095k. The average egg diameter from fertilization until final hatching was 763.49 plus or minus 0.00l6k. The average egg diameter was 751.81 plus or minus 0.0064k in murola stage, 767.55 plus or minus 0.0074k in nurula stage, 779.97 plus or minus 0.0084k in appearance of heart stage and 780.84 plus or minus 0.0086k in the increasing of pigmentation stage. Duration of egg incubation and embryo development of yellow fin seabream (Acanthopagrus latus) was 31 hours and 15 minutes at 20clC and 26 hours and 15 minutes at 23 plus or minus 1C. In this study, 20 stages of embryo development of yellow fin seabream were identified through incubation period from fertilization to final hatching. It seems that water temperature can be an effective environmental factor in the fish embryo development, so that hatching at 20clC occurred in 5 hours later than when at 23clC. Temperature change had no effect on the number of larvae and characteristics of embryo development. The information gathered through this study is useful when planning for artificial reproduction of this commercial species and can improve the propagation process

A novel technique for the formation of embryoid bodies inside liquid marbles

The significant and inherent limitations associated with commonly used methods of in vitro embryoid body (EB) formation motivate the development of novel, facile, efficient and reproducible techniques. In this study we report the possibility of using ''liquid marbles'' as facile and efficient micro bioreactors for in vitro EB formation. To exploit liquid marbles as micro biological reactors, embryonic stem cells (ES cells) were inoculated into liquid marbles containing embryonic cell growth medium. Herein we show how the confined internal space of a liquid marble, along with the porous and non-adhesive properties of the highly hydrophobic liquid marble shell, can provide the necessary conditions for the formation of uniform EBs inside liquid marbles. Factors such as the powder particle size, the liquid marble volume and the cell seeding density inside each liquid marble were also investigated to evaluate the effects of varying experimental conditions on the efficiency of EB formation within a liquid marble

Digestive enzymes activity and growth indices of rainbow trout (Oncorhynchus mykiss) fed diets supplemented with silymarin and Nickle Oxide nanoparticles

There is growing concern regarding nano-sized material discharge into water bodies and their subsequent toxicity to aquatic lives owing to increasingly rapid development and industrial applications of nanoparticles. This study evaluates the oral prescription of silymarin and Nickel Oxide nanoparticles in rainbow trout with an emphasis on growth indices and digestive enzymes activity. To that end, 1200 fish (3.83±0.01g) were randomly allotted into 8 distinct treatments including control group without any supplemental dietary Nickel Oxide nanopartcles or silymarin and the remaining seven experimental groups comprised of different combinations of Nickel Oxide nanoparticles (0, 100 and 500 mg /kg feed) and silymarin (0 and 1 g /kg feed) in the first and second month of the trial. All treatments were carried out in trplicate and the experiment lasted for 60 days. Results showed that the highest amylase activity was recorded in treatment 6 (16.56±1.00) (0 mg Nickel nanoparticle along with 1 g silymaryn - 500 mg Nickel nanoparticle and 1 g silymarin) which significantly differed from treatments 5 (0 mg Nickel Oxide nanoparticles and 1 mg silymarin-100 mg Nickel Oxide nanoparticles with 1 mg silymarin), 7 and 8 (fed diets containing 100 and 500 mg Nickel Oxide nanoparticles, respectively) (P≤0.05). The highest alkaline protease activity was observed in treatment 1 (0.54±0.05) (without any supplemental Nickel or silymarin), which was significantly different from those of treatments 7 and 8 (P≤0.05). The highest lipase activity was reported for treatment 4 (1.03±0.04) (500 mg Nickel nanoparticle with 1 g silymaryn- 0 mg nanoparticle and 1 g silymarin) which was significantly different from other treatments (P≤0.05). The results showed that simultaneous use of Nickel nanoparticle and silymarin in treatments 3, 4, 5 and 6 led to higher digestive enzymes activities in comparison to treatments 7 and 8. However, growth indices did not show any noticeable differences amongst studied treatments. It seems that in a long term exposure to Nickel Oxide nanoparticles and simoltanoues dietary silymarin inclusion, it would also be possible to observe differences in growth and nutritional indices, requiring further clarification

Surface-functionalization of PDMS for potential micro-bioreactor and embryonic stem cell culture applications

This study presents a novel and inexpensive method to prepare a disposable micro-bioreactor for stem cell expansion. The micro-bioreactor was fabricated in the form of a fixed bed bioreactor with a microchannel reactor bed. The micro-bioreactor was constructed from polydimethylsiloxane (PDMS), and the microchannel was functionalized to enable cell adhesion and resistance to bovine serum albumin protein adsorption. The PDMS reactor bed surface was activated by oxygen plasma, then aminized with trimethoxysilylpropyl(polyethyleneimine), followed by grafting with carboxylmethyl cellulose (CMC) and gelatin in sequence. The functionalized PDMS surface demonstrated improved hydrophilicity and antifouling properties. The grafting of gelatin promoted cell adhesion. The functionalized surface was found to be biocompatible with MDA-MB-231 and Oct4b2 cells and was demonstrated to facilitate cell proliferation. The expanded Oct4b2 cells retained their proliferation potential, undifferentiated phenotype and pluripotency

Estimating the completeness of lung cancer registry in Ardabil, Iran with a three-source capture-recapture method

Cancer registration is an important component of a comprehensive cancer control program, providing timely data and information for research and administrative use. Capture-recapture methods have been used as tools to investigate completeness of cancer registry data. This study aimed to estimate the completeness of lung cancer cases registered in Ardabil Population Based Cancer Registry (APBCR) with a three-source capture-recapture method. Data for all new cases of lung cancer reported by three sources (pathology reports, death certificates, and medical records) to APBCR for 2006 and 2008 were obtained. Duplicate cases shared among the three sources were identified based on similarity of first name, last name and father's names. A log-linear model was used to estimate number of missed cases and to control for dependency among sources. A total of 218 new cases of lung cancer was reported by three sources after removing duplicates. The estimated completeness calculated by log-linear method was 26.4 for 2006 and 27.1 for 2008. The completeness differed according to gender. In men, the completeness was 26.0 for 2006 and 28.1 for 2008. In women, the completeness was 36.5 for 2006 and 46.9 for 2008. In conclusion, none of the three sources can be considered as a reliable source for accurate cancer incidence estimation. © 2016, Asian Pacific Journal of Cancer Prevention

Relationship between urbanization and cancer incidence in Iran using quantile regression

Quantile regression is an efficient method for predicting and estimating the relationship between explanatory variables and percentile points of the response distribution, particularly for extreme percentiles of the distribution. To study the relationship between urbanization and cancer morbidity, we here applied quantile regression. This cross-sectional study was conducted for 9 cancers in 345 cities in 2007 in Iran. Data were obtained from the Ministry of Health and Medical Education and the relationship between urbanization and cancer morbidity was investigated using quantile regression and least square regression. Fitting models were compared using AIC criteria. R (3.0.1) software and the Quantreg package were used for statistical analysis. With the quantile regression model all percentiles for breast, colorectal, prostate, lung and pancreas cancers demonstrated increasing incidence rate with urbanization. The maximum increase for breast cancer was in the 90th percentile (β=0.13, p-value < 0.001), for colorectal cancer was in the 75th percentile (β=0.048, p-value < 0.001), for prostate cancer the 95th percentile (β=0.55, p-value < 0.001), for lung cancer was in 95th percentile (β=0.52, p-value=0.006), for pancreas cancer was in 10th percentile (β=0.011, p-value < 0.001). For gastric, esophageal and skin cancers, with increasing urbanization, the incidence rate was decreased. The maximum decrease for gastric cancer was in the 90th percentile(β=0.003, p-value < 0.001), for esophageal cancer the 95th (β=0.04, p-value=0.4) and for skin cancer also the 95th (β=0.145, p-value=0.071). The AIC showed that for upper percentiles, the fitting of quantile regression was better than least square regression. According to the results of this study, the significant impact of urbanization on cancer morbidity requirs more effort and planning by policymakers and administrators in order to reduce risk factors such as pollution in urban areas and ensure proper nutrition recommendations are made. © 2016, Asian Pacific Journal of Cancer Prevention

Estimating the completeness of gastric cancer registration in Ardabil/Iran by a capture-recapture method using population-based cancer registry data

Background: Knowledge of cancer incidences is essential for cancer prevention and control programs. Capture-recapture methods have been recommended for reducing bias and increasing the accuracy of cancer incidence estimations. This study aimed to estimate the completeness of gastric cancer registration by the capture-recapture method based on Ardabil population-based cancer registry data. Materials and Methods: All new cases of gastric cancer reported by three sources, pathology reports, death certificates and medical records that reported to Ardabil population-based cancer registry in 2006 and 2008 were enrolled in the study. The duplicate cases based on the similarity of first name, surname and fathers names were identified between sources. The estimated number of gastric cancers was calculated by the log-linear method using Stata 12 software. Results: A total of 857 new cases of gastric cancer were reported from three sources. After removing duplicates, the reported incidence rates for the years 2006 and 2008 were 35.3 and 32.5 per 100,000 population, respectively. The estimated completeness calculated by log-linear method for these years was 36.7 and 36.0, respectively. Conclusions: These results indicate that none of the sources of pathology reports, death certificates and medical records individually or collectively fully cover the incident cases of gastric cancer. We can obtain more accurate estimates of incidence rates using the capture-recapture method

Estimating the completeness of lung cancer registry in Ardabil, Iran with a three-source capture-recapture method

Cancer registration is an important component of a comprehensive cancer control program, providing timely data and information for research and administrative use. Capture-recapture methods have been used as tools to investigate completeness of cancer registry data. This study aimed to estimate the completeness of lung cancer cases registered in Ardabil Population Based Cancer Registry (APBCR) with a three-source capture-recapture method. Data for all new cases of lung cancer reported by three sources (pathology reports, death certificates, and medical records) to APBCR for 2006 and 2008 were obtained. Duplicate cases shared among the three sources were identified based on similarity of first name, last name and father's names. A log-linear model was used to estimate number of missed cases and to control for dependency among sources. A total of 218 new cases of lung cancer was reported by three sources after removing duplicates. The estimated completeness calculated by log-linear method was 26.4 for 2006 and 27.1 for 2008. The completeness differed according to gender. In men, the completeness was 26.0 for 2006 and 28.1 for 2008. In women, the completeness was 36.5 for 2006 and 46.9 for 2008. In conclusion, none of the three sources can be considered as a reliable source for accurate cancer incidence estimation. © 2016, Asian Pacific Journal of Cancer Prevention

Estimating the esophagus cancer incidence rate in Ardabil, Iran: A capture-recapture method

Background: Accurate cancer registry and awareness of cancer incidence rate is essential in order to define strategies for cancer prevention and control programs. Capture-recapture methods have been recommended for reducing bias and increase the accuracy of cancer incidence estimation. Objectives: This study aimed to estimate the esophagus cancer incidence by capture-recapture method based on Ardabil population-based cancer registry data. Patients and Methods: Total new cases of esophagus cancer reported by three sources of pathology reports, medical records, and death certificates to Ardabil province cancer registry center in 2006 and 2008 were enrolled in the study. All duplicated cases between three sources were identified and removed using Excel software. Some characteristics such as name, surname, father�s name, date of birth and ICD codes related to their cancer type were used for data linkage and finding the common cases among three sources. The incidence rate per 100,000 was estimated based on capture-recapture method using the log-linear models. We used BIC, G2 and AIC statistics to select the best-fit model. Results: After removing duplicates, total 471 new cases of esophagus cancer were reported from three sources. The model with linkage between pathology reports, medical record sources and independence with the death certificates source was the best fitted model. The reported incidence rate for the years 2006 and 2008 was 18.77 and 18.51 per 100,000, respectively. In log-linear analysis, the estimated incidence rate for the years 2006 and 2008 was 49.71 and 53.87 per 100,000 populations, respectively. Conclusions: Based on the obtained results, it can be concluded that none of the sources of pathology reports, death certificates and medical records individually or collectively were fully covered the incidence cases of esophagus cancer and need to apply some changes in data abstracting and case finding. © 2016, Iranian Journal of Cancer Prevention