HAEMATO-BIOCHEMICAL PROFILE AND MILK LEUKOCYTE COUNT IN SUBCLINICAL AND CLINICAL MASTITIS AFFECTED CROSSBRED CATTLE Journal of Experimental Biology and Agricultural Sciences

ABSTRACT The study was conducted to determine the effect of subclinical mastitis (SCM) and clinical mastitis (CM) on haemato-biochemical parameters and milk leukocyte count of crossbred cattle. Milk and blood samples were collected from 20 healthy, 223 SCM and 47 CM affected animals from Kolar and Chikkaballapur districts of Karnataka state, India. The SCM were diagnosed by California Mastitis Test (CMT) and electrical conductivity (EC) of milk. Blood and milk of mastitis infected crossbred cattle were analyzed for haemato-biochemical parameters and milk leukocyte count. Haematology showed significant increase in TLC in crossbred cows affected with mastitis. Significantly (P< 0.05) lower average values of TEC, Hb and PCV were observed in SCM infected animals, however no significant change were observed in values of CM infected than healthy animals. Differential leucocytic count (DLC) revealed higher granulocytes and lymphopenia in mastitis infected animals. Biochemical estimation revealed significantly (P< 0.05) higher average values of Ca, P, Na, Cl, and K in mastitis infected compared with healthy animals however, no significant (P> 0.05) change was observed in Mg and ALT levels. AST and TP values were significantly increased in SCM infected compared to healthy animals however, no significant change were observed in CM infected animals

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Not AvailableOvine Johne's disease (OJD) is caused by Mycobacterium avium sub sp. paratuberculosis (MAP). The disease presents diverse bacteriological, immunological and pathological spectra leading to variable efficacies of diagnostic methods at different times during the course of infection. The study was carried out to evaluate the sensitivity and specificity of indirect-enzymelinked immunosorbent assay (ELISA) using IS900 faecal polymerase chain reaction (PCR) assay as gold standard. A total of 100 clinical samples, serum (n = 50) and faeces (n = 50), were collected from 50 adult Bannur sheep from Livestock Research and Information Centre farm Nagamangala, Mandya district of Karnataka. Of 100 total samples, 23 (23%) serum and 44 (44%) faecal samples were positive by indirect-ELISA and IS900 PCR assay, respectively. The sensitivity and specificity of indirect-ELISA compared with IS900 PCR assay was 50% and 98.21%, respectively. Kappa and area under curve value indicates moderate agreement between indirect-ELISA and IS900 faecal PCR assay. The present study indicated that IS900 faecal PCR was more sensitive and specific test than indirect-ELISA in detecting for MAP infection and for early diagnosis of JD in sheep. Indirect-ELISA was screening test and IS900 PCR assay was an individual confirmatory test. Combination of indirect-ELISA with IS900 faecal PCR assay may be adopted as a model strategy for screening and diagnosis of JD in sheepNot Availabl