Influence of Supplementing Sesbania grandiflora Pod Meal at Two Dietary Crude Protein Levels on Feed Intake, Fermentation Characteristics, and Methane Mitigation in Thai Purebred Beef Cattle

The aim of the study was to evaluate the effect of crude protein (CP) levels in concentrate and Sesbania grandiflora pod meal (SG) supplementation on feed intake, rumen fermentation, and methane (CH4) mitigation in Thai purebred beef cattle. Four cattle with 100 ± 5.0 kg body weight were used in this study. A 2 × 2 factorial experiment in a 4 × 4 Latin square design was conducted, in which factor A was the CP levels in concentrate of 14% and 16% of dry matter (DM) and factor B was the supplement levels of SG at 0.4% and 0.6% DM intake, respectively. The results showed that the CP content in concentrate and SG supplementation had no interaction effect on intake, digestibility, ruminal ecologies, ruminal fermentation products, and nitrogen utilization. Increasing CP content to 16% significantly (p < 0.05) increased the ruminal ammonia nitrogen (NH3-N), nitrogen (N) intake, N absorption, and N retention. SG supplementation significantly (p < 0.05) decreased CP digestibility, NH3-N, blood urea nitrogen, and protozoa. In addition, SG significantly decreased acetate (C2), acetate to propionate ratio, methane, and fecal N excretion, while it significantly increased total volatile fatty acids (VFAs) and propionate (C3) concentration. In conclusion, SG could mitigate methane emission and enhance nitrogen utilization

Effect of Feeding Discarded Durian Peel Ensiled with <i>Lactobacillus casei TH14</i> and Additives in Total Mixed Rations on Digestibility, Ruminal Fermentation, Methane Mitigation, and Nitrogen Balance of Thai Native–Anglo-Nubian Goats

The objective of this study was to evaluate the effect of fermented discarded durian peel with Lactobacillus casei TH14, cellulase, and molasses separately or in combination in total mixed rations on feed utilization, digestibility, ruminal fermentation, and nitrogen utilization in growing crossbreed Thai Native–Anglo-Nubian goats. Five crossbreed Thai Native–Anglo-Nubian goats (50%) at 9 to 12 months of age and 20 ± 1 of body weight (BW) were assigned to a 5 × 5 Latin square design. Evaluated treatments were fermented discarded durian peel without additives (FDP), fermented discarded durian peel with 5% of molasses (FDPM), fermented discarded durian peel with 2% of cellulase (FDPC), fermented discarded durian peel with 1.0 × 105 cfu/g fresh matter of L. casei TH14 (FDPL), and fermented discarded durian peel with 5% of molasses and 1.0 × 105 cfu/g fresh matter of L. casei TH14 (FDPML). This study showed that acid detergent fiber intake was different (p p p L. casei TH14 in combination could add 25% of dry matter into the diet for growing goats without a negative impact

Using glycerin with chitosan extracted from shrimp residue to enhance rumen fermentation and feed use in native Thai bulls

Background and Aim: Crude glycerin is changed to propionate in the rumen, while chitosan can be used as a feed supplement to increase propionic acid concentration and decrease methane (CH4) production. We hypothesized that supplementation with a combination of a high level of crude glycerin with chitosan could have a beneficial effect on ruminal fermentation and mitigate CH4 production. This study aimed to explore the combined effects of crude glycerin and chitosan supplementation on nutrient digestibility, rumen fermentation, and CH4 calculation in native Thai bulls. Materials and Methods: Four 2-year-old native Thai bulls, weighing 150±20 kg, were kept in a 2×2 factorial arrangement in a 4×4 Latin square design. Factor A represented the incorporation of crude glycerin at 10.5% and 21% of the dry matter (DM) of a total mixed ration (TMR), and factor B represented the supplementation of chitosan at 1% and 2% DM of a TMR. Results: Increasing levels of crude glycerin at 21% decreased DM intake by 0.62 kg/day compared with 10.5% crude glycerin (p0.05). The incorporated crude glycerin and supplemented chitosan levels did not affect the pH, temperature, concentrations of ammonia-nitrogen, microbial population, and blood urea nitrogen (p>0.05). Supplemented chitosan and incorporated crude glycerin did not show any interaction effects on the molar portions and total volatile fatty acids (VFAs), except estimated CH4. Increasing the incorporated crude glycerin levels increased propionate and decreased the ratio of acetate to propionate ratio, whereas levels of butyrate, acetate, and total VFAs were unchanged. The combination of crude glycerin at 21% in the TMR with chitosan at 2% reduced CH4 estimation by 5.08% compared with the other feed treatment. Conclusion: Increasing incorporated crude glycerin levels in a TMR significantly elevated the propionate concentration, whereas combining 21% crude glycerin in the TMR diet with 2% chitosan supplementation could depress CH4 estimation more effectively than adding one of these supplements alone

Effect of Feeding Discarded Durian Peel Ensiled with Lactobacillus casei TH14 and Additives in Total Mixed Rations on Digestibility, Ruminal Fermentation, Methane Mitigation, and Nitrogen Balance of Thai Native&ndash;Anglo-Nubian Goats

The objective of this study was to evaluate the effect of fermented discarded durian peel with Lactobacillus casei TH14, cellulase, and molasses separately or in combination in total mixed rations on feed utilization, digestibility, ruminal fermentation, and nitrogen utilization in growing crossbreed Thai Native&ndash;Anglo-Nubian goats. Five crossbreed Thai Native&ndash;Anglo-Nubian goats (50%) at 9 to 12 months of age and 20 &plusmn; 1 of body weight (BW) were assigned to a 5 &times; 5 Latin square design. Evaluated treatments were fermented discarded durian peel without additives (FDP), fermented discarded durian peel with 5% of molasses (FDPM), fermented discarded durian peel with 2% of cellulase (FDPC), fermented discarded durian peel with 1.0 &times; 105 cfu/g fresh matter of L. casei TH14 (FDPL), and fermented discarded durian peel with 5% of molasses and 1.0 &times; 105 cfu/g fresh matter of L. casei TH14 (FDPML). This study showed that acid detergent fiber intake was different (p &lt; 0.05) between goats fed FDP and those fed FDPLM, 0.24 g/d and 0.20 g/d, respectively. The FDPML ration had significantly (p &lt; 0.05) greater apparent nutrient digestibility and a better propionate concentration compared with other treatments. FDPML treatment significantly (p &lt; 0.05) decreased the acetate-to-propionate ratio, methane production, and urinary nitrogen. Therefore, treated discarded durian peel with molasses and L. casei TH14 in combination could add 25% of dry matter into the diet for growing goats without a negative impact

Megasphaera elsdenii and Saccharomyces Cerevisiae as direct fed microbials during an in vitro acute ruminal acidosis challenge

This study aimed to evaluate the efects of Saccharomyces cerevisiae and Megasphaera elsdenii as direct fed microbials (DFM) in beef cattle fnishing diets to alleviate acute ruminal lactic acidosis in vitro. A dual-fow continuous culture system was used. Treatments were a Control, no DFM; YM1, S. cerevisiae and M. elsdenii strain 1; YM2, S. cerevisiae and M. elsdenii strain 2; and YMM, S. cerevisiae and half of the doses of M. elsdenii strain 1 and strain 2. Each DFM dose had a concentration of 1 × ­108 CFU/mL. Four experimental periods lasted 11 days each. For the non-acidotic days (day 1–8), diet contained 50:50 forage to concentrate ratio. For the challenge days (day 9–11), diet contained 10:90 forage to concentrate ratio. Acute ruminal acidosis was successfully established. No diferences in pH, d-, l-, or total lactate were observed among treatments. Propionic acid increased in treatments containing DFM. For N metabolism, the YMM treatment decreased protein degradation and microbial protein synthesis. No treatment efects were observed on NH3–N concentration; however, efciency of N utilization by ruminal bacteria was greater than 80% during the challenge period and NH3–N concentration was reduced to approximately 2 mg/dL as the challenge progressed.UCR::Vicerrectoría de Docencia::Ciencias Agroalimentarias::Facultad de Ciencias Agroalimentarias::Escuela de Zootecni

Production, physiological response, and calcium and magnesium balance of lactating Holstein cows fed different sources of supplemental magnesium with or without ruminal buffer

The objective of this study was to evaluate the effects of dietary replacement of magnesium oxide (MgO) with calcium-magnesium hydroxide [CaMg(OH)2] and its interaction with ruminal buffer (sodium sesquicarbonate) supplementation on production, Ca and Mg balance, and overall physiological response of mid-lactation Holstein dairy cows. Sixty cows averaging 40.5 ± 7.0 kg of milk/d were used. Treatments were assigned following a 2 × 2 factorial arrangement: (1) MgO, (2) MgO + buffer, (3) CaMg(OH)2, or (4) CaMg(OH)2 + buffer. Diets were formulated to have 16.5% of crude protein, 1.82 Mcal/kg of net energy for lactation, 0.67% Ca, 0.39% P, and 0.25% Mg, all on a dry matter (DM) basis. Treatments were individually top dressed. Milk production, composition, and DM intake were evaluated. A subsample of 20 cows were randomly selected for the evaluation of Ca and Mg balance, blood gases, and electrolytes. Ruminal fluid was also collected for evaluation of pH and Ca and Mg solubility. Effects of Mg source, buffer, and the interaction Mg source × buffer were analyzed through orthogonal contrasts. An interaction of Mg source × buffer was found for DM intake and feed efficiency, in which cows fed CaMg(OH)2 had a similar feed efficiency regardless of ruminal buffer inclusion; however, when cows were fed MgO, the inclusion of buffer reduced feed efficiency. No effects on body weight and milk yield were observed. Buffer addition tended to increase the concentrations of fat, protein, and solids-not-fat, without affecting the yields of these milk components. Magnesium source and buffer did not affect ruminal fluid, blood, urine, or fecal pH; however, buffer supplementation increased urinary pH. Treatment with CaMg(OH)2 increased blood concentration of HCO3 −, total CO2, and base excess compared with cows fed MgO. No differences were observed in the ruminal solubility of Ca and Mg or on milk or urinary Ca and Mg excretion. Greater plasma Mg concentration was observed for animals fed MgO compared with cows fed CaMg(OH)2; however, both sources were above the threshold recommended in the literature for dairy cows. Also, a reduction in fecal Mg excretion was observed in animals fed CaMg(OH)2. In summary, we provide evidence that CaMg(OH)2 could replace MgO without affecting performance, overall physiological response, or Ca and Mg balance of mid-lactating dairy Holstein cows.UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro de Investigación en Nutrición Animal (CINA)UCR::Vicerrectoría de Docencia::Ciencias Agroalimentarias::Facultad de Ciencias Agroalimentarias::Escuela de Zootecni