ent-Clerodane Diterpenes from the Bark of Croton oligandrus Pierre ex. Hutch. and Assessment of their Cytotoxicity Against Human Cancer Cell Lines

New clerodane diterpenes, 12-epi-megalocarpodolide D (2) and an epimeric mixture of crotonolins A (3) and B (4), were isolated from the bark of Croton oligandrus following a bioassay-guided isolation protocol. Known compounds, megalocarpodolide D (1), 12-epi-crotocorylifuran (5), cluytyl-ferulate (6), hexacosanoyl- ferulate (7), vanillin (8), acetyl-aleuritolic acid (9) and lupeol (10), were also isolated. The structures of the isolated compounds (1-10) were elucidated by spectroscopic means. The cytotoxicity of compounds 1-10 was assessed against A549, MCF7, PC3 and PNT2 cell lines using the MTT assay. Compounds 1 and 2 showed moderate level of activity against both A549 and MCF7 cells with 1 being the most active with IC50 values of 63.8±13.8 and 136.2±22.7 µM against A549 and MCF7 cells, respectively. The epimeric mixture of 3 and 4 was moderately active against A549 and PC3 cells (IC50 = 128.6±31.0 and 111.2±2.9 µM, respectively)

Prevalence, determinants and health care-seeking behavior of childhood acute respiratory tract infections in Bangladesh

Background: Acute respiratory infections (ARIs) are one of the leading causes of child mortality worldwide and contribute significant health burden for developing nations such as Bangladesh. Seeking care and prompt management is crucial to reduce disease severity and to prevent associated morbidity and mortality.Objective: This study investigated the prevalence and care-seeking behaviors among under-five children in Bangladesh and identified factors associated with ARI prevalence and subsequent care-seeking behaviors.Method: The present study analyzed cross-sectional data from the 2014 Bangladesh Demographic Health Survey. Bivariate analysis was performed to estimate the prevalence of ARIs and associated care-seeking. Logistic regression analysis was used to determine the influencing socio-economic and demographic predictors. A p-value of <0.05 was considered as the level of significance.Result: Among 6,566 under-five children, 5.42% had experienced ARI symptoms, care being sought for 90% of affected children. Prevalence was significantly higher among children < 2 years old, and among males. Children from poorer and the poorest quintiles of households were 2.40 (95% CI = 1.12, 5.15) and 2.36 (95% CI = 1.06, 5.24) times more likely to suffer from ARIs compared to the wealthiest group. Seeking care was significantly higher among female children (AOR = 2.19, 95% CI = 0.94, 5.12). The likelihood of seeking care was less for children belonging to the poorest quintile compared to the richest (AOR = 0.03, 95% CI = 0.01, 0.55). Seeking care from untrained providers was 3.74 more likely among rural residents compared to urban (RRR = 3.74, 95% CI = 1.10, 12.77).Conclusion: ARIs continue to contribute high disease burden among under-five children in Bangladesh lacking of appropriate care-seeking behavior. Various factors, such as age and sex of the children, wealth index, the education of the mother, and household lifestyle factors were significantly associated with ARI prevalence and care-seeking behaviors. In addition to public-private actions to increase service accessibility for poorer households, equitable and efficient service distribution and interventions targeting households with low socio-economic status and lower education level, are recommended

Zanthoamides G-I: Three new alkamides from Zanthoxylum zanthoxyloides

Three new alkamides, zanthoamides G-I (1-3), together with ten known compounds, araliopsine, skimmianine, N-methylplatydesminium cation, isoplatydesmine, myrtopsine, atanine, N-methylatanine, sesamin, hesperetin and hesperidin, were isolated from the fruits of Zanthoxylum zanthoxyloides. Their structures were elucidated by spectroscopic means. All isolated compounds were assessed for their cytotoxicity against A549, MCF7, PC3 and PNT2 cell lines. Among the alkamides, only zanthoamide G (1) showed low level of cytotoxicity against MCF7 cells

Bioassay-Guided Isolation and Structure Elucidation of Cytotoxic Stilbenes and Flavonols from the Leaves of Macaranga barteri

Bioassay-guided fractionation of the leaves of Macaranga barteri collected from Nigeria led to the isolation of three previously undescribed cytotoxic stilbenes, macabartebenes A-C (1-3), together with six known compounds including prenylated stilbenes: vedelianin (4), schweinfurthin G (5), and mappain (7), prenylated flavonols: 8-prenylkaempferol (6), and broussoflavonol F (8), and the geranylated flavonol, isomacarangin (9). The cytotoxicity of the compounds was evaluated against four human cancer cell lines, with vinblastine as the positive control and DMSO vehicle as the negative control. Vedelianin (IC50 = 0.32 – 0.54 μM) displayed the greatest antiproliferative activity across the panel of cancer cell lines amongst the compounds, while macabartebene A (IC50 = 0.60 – 0.79 μM) was the most potent of the previously unreported compounds. The compounds displayed varying selectivity towards the cancer cell lines compared to the normal human prostate cell line. The findings of this study revealed that M. barteri leaves contain several cytotoxic compounds

Four new neo-clerodane diterpenes from the stem bark of Croton oligandrus.

Four new neo-clerodanes, crotonolins C-F (3-6), were isolated from the stem bark of Croton oligandrus together with the known clerodane crotonzambefuran A, the abietanes 7-β-hydroxydehydroabietic acid and 7-oxodehydroabietic acid, and ferulic acid. Their structures were elucidated by spectroscopic analyses including 1D and 2D NMR and HRESIMS and by comparison with previously reported data. The cytotoxicity of the isolated compounds against A549, MCF7, PC3 and PNT2 cells was evaluated using the MTT assay. Only 7-β-hydroxydehydroabietic acid showed a moderate level of activity against PC3 cells with an IC50 value of 68.9 ± 6.6 μM

Cytotoxic properties of the stem bark of Citrus reticulata Blanco (Rutaceae)

The bioassay-guided fractionation of the n-hexane extract of Citrus reticulata Blanco (Rutaceae) stem bark yielded scoparone (1), xanthyletin (2), lupeol (3), β-amyrin (4), stigmasterol (5), β-sitosterol (6) and palmitic acid. The structures of these compounds were determined by comprehensive spectroscopic analyses, i.e., 1D and 2D NMR and EI-MS, and by comparison with the reported data. Extracts, fractions and isolated compounds 1-6 were assessed for cytotoxicity by the MTT assay against three human cancer cell lines, i.e., human lung adenocarcinoma cell line A549, human breast adenocarcinoma cell line MCF7 and human Caucasian prostate adenocarcinoma cell line PC3. Significant activity of the n-hexane and the dichloromethane extracts was observed against the breast cancer cell line MCF7 with IC50s of 45.6 and 54.7 μg/mL, respectively. Moreover, the 70% ethyl acetate in n-hexane chromatographic fraction showed significant activity displaying IC50 values of 53.0, 52.4 and 49.1 μg/mL against the cancer cell lines A549, MCF7 and PC3, respectively. Encouragingly, an IC50 of 510.0 µg/mL against the human normal prostate cell line PNT2 indicated very low toxicity, and hence favourable selectivity indices for the 70% ethyl acetate in n-hexane fraction in the range of 9.6-10.4 towards cell lines A549, MCF7 and PC3. Since compounds isolated from the above fraction only delivered IC50 values in the range of 18.2-96.3, 9.2-34.1 and 7.5-97.2 μg/mL against A549, MCF7 and PC3 cell lines, respectively, synergistic action between compounds is suggested. Bioassay results valorize the anticancer effectivity of the stem bark of this plant in Cameroonian pharmacopeia

Growth inhibitory activity of biflavonoids and diterpenoids from the leaves of the Libyan Juniperus phoenicea against human cancer cells

Three biflavonoids [cupressuflavone (1), amentoflavone (2)and sumaflavone (3)], four diterpenoids [13-epi-cupressic acid (4), imbricatholic acid (5), 3-hydroxy-sandaracopimaric acid (6)and dehydroabietic acid (7)]and onelignan [β-peltatin methyl ether (8)],were isolated from the cytotoxicfractions of the extracts of the leaves of the Libyan Juniperus phoeniceaL. The structures of these compounds were elucidated by spectroscopic means.Cytotoxicity of the compounds 1-6were assessedagainst the human lung cancer cell lineA549 using the MTT assay. Compounds 1and 3showed cytotoxicityagainst the A549cells(IC50= 65 µMand 77 µM, respectively), whereas, compound 2did not show any activity. Diterpenes4-6exhibited weak cytotoxicity against the A549 cells with the IC50values of 159 µM, 263 µMand 223 µM, respectively. The cytotoxicity of each compound was compared with the anticancer drug,etoposide (IC50=61 µM).Cupressuflavone (1)wasevaluatedalso for cytotoxicity against both the human PC3 cancer cell lineand the normal prostatecell line (PNT2), and this compoundrevealed a high degreeof cytotoxic selectivity towards the prostate cancer cells (PC3), with IC50value of 19.9 µM, without any evidence of cytotoxicity towards the normal prostatecell line(PNT2)

Economic burden of hospitalized diarrheal disease in Bangladesh

Diarrheal diseases are a major threat to human health and still represent a leading cause of mortality and morbidity worldwide. Although the burden of the diarrheal diseases is much lower in developed countries, it is a significant public health problem in low and middle-income countries like Bangladesh. The aim of the study is to capture the inpatients and outpatient treatment cost of diarrheal disease and to measure the cost burden and coping mechanisms associated with diarrheal illness

Histomorphological Study of Umbilical Cord on 50 Bangladeshi

Microscopic study was done on 50 human umbilical cords in the Department of Anatomy, during January to December 1998 at Bangabandhu Sheikh Mujib Medical University, Dhaka. The basic structure of the umbilical cord was observed. The covering of umbilical cord varied on two sides. The side overlying the umbilical vein was covered by simple epithelium and that overlying the umbilical arteries was covered by multilayer epithelium. Presence of internal elastic lamina of umbilical arteries was found in 18% and in the veins was in 70% cases. Remnant of allantois was found in 28% cases