1 research outputs found
Somatic embryo-like structures of strawberry regenerated <i style="mso-bidi-font-style:normal">in vitro</i> on media supplemented with 2,4-D and BAP
739-745Somatic embryo-like structures (SELS) were produced in vitro from leaf disk and petiole
explants of two cultivars of strawberry (Fragaria
Ă— ananassa Duch) on Murashige and
Skoog medium with different concentrations and combinations of 2,4‑dichlorophenoxyacetic
acid (2,4-D), 6-benzylaminopurine (BAP) and sucrose to check the embryonic
nature of these structures histologically. A large number of SELS could be
regenerated in both cultivars on media with 2 - 4 mg L-1 2,4-D in
combination with 0.5 - 1 mg L-1 BAP and 50 g L-1
sucrose. Histological examination of SELS revealed the absence of a root pole.
Therefore these structures cannot be strictly classified as somatic embryos.
The SELS formed under the tested culture conditions represent malformed
shoot-like and leaf-like structures. The importance of these results for the
propagation of strawberries via somatic embryogenesis is discussed