Flowable bulk-fill materials compared to nano ceramic composites for class I cavities restorations in primary molars: A two-year prospective case-control study

Background: The aim of this split-mouth study is to compare the results of 24 months’ clinical performance of primary molar Class I restorations with a nano-ceramic composite, Ceram•X mono (Dentsply) with a flowable bulk-fill material regular viscosity, SDR (Dentsply). Methods: Following the ethical approval, 27 patients with at least two class I cavities in primary molars were included in the study. A total number of 54 restorations were conducted (n = 27 for Ceram X and n = 27 for SDR). Restorations were evaluated at baseline, 6, 18, and 24 months, according to the modified Ryge criteria. The cavosurface marginal discoloration and color match were evaluated visually after air-drying the tooth and after removing the plaque (if necessary). Results: At 24 months’ follow-up, 54 restorations showed similar clinical performance. The statistical analysis did not reveal any statistical significance in the values between the groups in 7 out of 7 modified Ryge criteria. However, two restorations in both groups received Bravo ratings in the cavosurface marginal discoloration scoring. No side effects were reported by the participants of the study. Conclusion: Restorations with both materials (Ceram•X mono and SDR) have provided almost identical results. © 2019 by the authors.Government Council on Grants, Russian Federation: 02.AAAA-A18-118020690020-1Funding: Partly the study was supported by the Government contract of Russian Federation with Institute of Immunology and Physiology (AAAA-A18-118020690020-1) and by the Act 211 of the Government of Russian Federation, contract No 02.A03.21.0006

Neurocirculatory dystonia or Da Costa's syndrome. The history of study

The review provides information about the main stages of the study of neurasthenia (da Costa syndrome) from the end of XIX to the XX century. The basic stages and approaches to studying the disease are described. In addition, the difference in approaches between the American, European, Eastern and Russian schools are revealed.В обзоре представлены сведения об основных этапах изучения нейроциркуляторной астении в период с конца XIX до XX века. Выделены основные этапы и подходы к изучению данного заболевания. Показана разница в подходах между американской, европейской,восточной и российской школах

Mononuclear phagocyte system. Development and activation of macrophages

Traditionally mononuclear phagocyte system was considered as a system that provides mostly the reactions of immune response, but more recently with enough data been accumulated, the understanding of its functions extended greatly. With the new experimental data, the change in ideas about the origin and even the composition of the cells in the system was occurred. The review is devoted to actual topic—the formation of mononuclear phagocyte system. Special attention is given to monocytes and macrophage's activation as the modulator of their future functions. The review article is based on the data from contemporary works.Традиционно система фагоцитирующих мононуклеаров рассматривается как система, обеспечивающая, прежде всего, реакции иммунного ответа, однако в последнее время накопилось достаточное количество данных, расширяющих представления о ее функциях. С появлением нового экспериментального материала, меняются и представления о происхождении и даже составе клеток, входящих в систему. Данный обзор посвящен основным особенностям образования клеток системы. Особое внимание уделяется свойствам и направлению развития моноцитов крови и системе активации макрофагов, как основному процессу, модулирующей их функции. Обзор базируется на данных экспериментальных и клинических работ

Effects of 1,3,4-thiadiazine compound with antidepressant properties in ligation model of acute pancreatitis

Based on hypotheses concerning the role of stress in acute pancreatitis development, the experimental approach for the decrease stress damage via the use the compound with proven antistress/neuroleptic action was conducted. The study was aimed to discover 2-morpholino-5-phenyl-6H-1,3,4-thiadiazine hydrobromide (compound L-17) therapeutic action in experimental acute pancreatitis. The experimental model used was the ligation model. The trial was carried out on 50 male Wistar rats with average body weight 180-240g. Histological picture of the pancreas was studied and biochemical and enzyme-immunoassays were carried out on the first and seventh days. The significant reduction in mortality on the background of L-17 compound administration was observed. While levels of all cytokines increased in induced experimental acute pancreatitis groups, the cytokine level rise was decreased when compound L-17 was administered. On the cellular level, the study revealed L-17’s ability to prevent granulocytosis and decrease granulocytes infiltration to inflammatory foci. The decrease in inflammatory reaction magnitude and prevention of abscess formation in experimental acute pancreatitis accompanied by sistemic inflamamtion was due to L-17’s ability to reduce neutrophilia and neutrophil entry into the injury zone. © 2018, Slovak Academy of Sciences. All rights reserved.Government Council on Grants, Russian FederationMinistry of Education and Science of the Russian Federation, Minobrnauka: 17.7255.2017/8.9AAAA-A18-118020690020-1Funding information. Partly the study was supported by the Act 211 of the Government of Russian Federation, contract No 02.A03.21.0006; Government contract of Russian Federation with Institute of Immunology and Physiology (AAAA-A18-118020690020-1) and the Ministry of Education and Science of the Russian Federation (# 17.7255.2017/8.9)

Pharmacologic Evaluation of Antidepressant Activity and Synthesis of 2-Morpholino-5-phenyl-6H-1,3,4-thiadiazine Hydrobromide

Substituted thiadiazines exert a reliable therapeutic effect in treating stress, and a schematic description of their ability to influence all aspects of a stress response has been depicted. This study was conducted to pharmacologically evaluate compound L-17, a substituted thiadiazine, (2-morpholino-5-phenyl-6H-1,3,4-thiadiazine, hydrobromide) for possible anti-psychotic/antidepressant activity. Compound L-17 was synthesized by cyclocondensation of α-bromoacetophenone with the original morpholine-4-carbothionic acid hydrazide. Pharmacologic evaluations were conducted using methods described by E.F. Lavretskaya (1985), and in accordance with published guidelines for studying drugs for neuroleptic activity. Compound L-17 was evaluated for various possible mechanisms of action, including its effects on cholinergic system agonists/antagonists, dopaminergic neurotransmission, the adrenergic system, and 5-HT3 serotonin receptors. One or more of these mechanisms may be responsible for the beneficial effects shown by thiadiazine compounds in experiments conducted to evaluate their activity in models of acute stress and acute myocardial infarction

Immunomodulatory Action of Substituted 1,3,4-Thiadiazines on the Course of Myocardial Infarction

This review focuses on the biological action of the compounds from the group of substituted 1,3,4-thiadiazines on stress response and myocardial infarction. The aim of this review is to propose the possible mechanisms of action of 1,3,4-thiadiazines and offer prospectives in the development of new derivatives as therapeutic agents. It is known, that compounds that have biological effects similar to those used as antidepressants can down-regulate the secretion of proinflammatory cytokines, up-regulate the release of anti-inflammatory ones and affect cell recruitment, which allows them to be considered immunomodulators as well. The results of pharmacological evaluation, in silico studies, and in vivo experiments of several compounds from the group of substituted 1,3,4-thiadiazines with antidepressant properties are presented. It is proposed that the cardioprotective effects of substituted 1,3,4-thiadiazines might be explained by the peculiarities of their multi-target action: the ability of the compounds to interact with various types of receptors and transporters of dopaminergic, serotonergic and acetylcholinergic systems and to block the kinase signal pathway PI3K-AKT. The described effects of substituted 1,3,4-thiadiazines suggest that it is necessary to search for a new agents for limiting the peripheral inflammatory/ischemic damage through the entral mechanisms of stress reaction and modifying pro-inflammatory cytokine signaling pathways in the brain

Psychological distress and post-traumatic symptomatology among dental healthcare workers in Russia: Results of a pilot study

The spread of SARS-CoV-2 infection has increased the risk of mental health problems, including post-traumatic stress disorders (PTSD), and healthcare workers (HCWs) are at greater risk than other occupational groups. This observational cross-sectional study aimed to explore the symptoms of depression, anxiety, and PTSD among dental HCWs in Russia during the coronavirus disease 2019 (COVID-19) pandemic. The survey was carried out among 128 dental HCWs from three dental clinics of Ekaterinburg, Russia. The mean age of the sample was 38.6 years. Depression, anxiety, and stress were assessed using the Depression Anxiety and Stress Scale-21 (DASS-21); PTSD was assessed using the PTSD Symptom Scale-Self-Report (PSS-SR); subjective distress was assessed using the Impact of Event Scale-Revised (IES-R). The results indicated that 20.3-24.2% HCWs had mild to extremely severe symptoms of psychological distress, and 7.1-29.7% had clinical symptoms of PTSD. No differences between females and males were revealed. HCWs working directly with patients had significantly higher levels of PTSD symptoms and the risk of PTSD development compared to those working indirectly, whereas older HCWs had significantly higher levels of both psychological distress and PTSD symptoms compared to younger HCWs. Thus, dental HCWs are at high risk for psychological distress and PTSD symptoms during the COVID-19 pandemic. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.The study was partly supported by the Government contract of the Institute of Immunology and Physiology (AAAA-A18-118020690020-1) and the HSE University Basic Research Program (funded by the Russian Academic Excellence Project 5-1000)

Combined in Silico, Ex Vivo, and in Vivo Assessment of L-17, a Thiadiazine Derivative with Putative Neuro-and Cardioprotective and Antidepressant Effects

Depression associated with poor general medical condition, such as post-stroke (PSD) or post-myocardial infarction (PMID) depression, is characterized by resistance to classical antidepres-sants. Special treatment strategies should thus be developed for these conditions. Our study aims to investigate the mechanism of action of 2-morpholino-5-phenyl-6H-1,3,4-thiadiazine, hydrobro-mide (L-17), a recently designed thiadiazine derivative with putative neuro-and cardioprotective and antidepressant-like effects, using combined in silico (for prediction of the molecular binding mechanisms), ex vivo (for assessment of the neural excitability using c-Fos immunocytochemistry), and in vivo (for direct examination of the neuronal excitability) methodological approaches. We found that the predicted binding affinities of L-17 to serotonin (5-HT) transporter (SERT) and 5-HT3 and 5-HT1A receptors are compatible with selective 5-HT serotonin reuptake inhibitors (SSRIs) and antagonists of 5-HT3 and 5-HT1A receptors, respectively. L-17 robustly increased c-Fos immunoreac-tivity in the amygdala and decreased it in the hippocampus. L-17 dose-dependently inhibited 5-HT neurons of the dorsal raphe nucleus; this inhibition was partially reversed by the 5-HT1A antagonist WAY100135. We suggest that L-17 is a potent 5-HT reuptake inhibitor and partial antagonist of 5-HT3 and 5-HT1A receptors; the effects of L-17 on amygdaloid and hippocampal excitability might be mediated via 5-HT, and putatively mediate the antidepressant-like effects of this drug. Since L-17 also possesses neuro-and cardioprotective properties, it can be beneficial in PSD and PMID. Combined in silico predictions with ex vivo neurochemical and in vivo electrophysiological assessments might be a useful strategy for early assessment of the efficacy and neural mechanism of action of novel CNS drugs. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Funding: The work of the authors of this study was supported by the Slovak Research and Development Agency (contract APVV-19-0435), Scientific Grant Agency of the Ministry of Education of the Slovak Republic, the Slovak Academy of Sciences (grant VEGA 2/0046/18), and a Government Contract of the Russian Federation with the Institute of Immunology and Physiology (AAAA-A18-118020690020-1)