ExCyto PCR amplification.

<p>A cDNA library was transformed into ExCyto cells. Twenty-four different cDNAs were amplified without addition of exogenous polymerase using (A) single colonies, (B) 2 µl of frozen glycerol stocks, or (C) 2 µl of overnight cultures. One kb molecular weight markers are shown in the last lane.</p

Dilution series of ExCyto PCR cells.

<p>Eight different clones from a cDNA library transformed into ExCyto cells were grown overnight, and a serial dilution was used for amplification (undiluted, 100-, and 1000-fold dilutions). In (A), bacteria were diluted, reducing both the target DNA and the tsDNA polymerase. In (B), to compensate for the serial dilution of the tsDNA polymerase, 2 ul of ExCyto cells (OD 1.1) without plasmids was added. In (C), to compensate for the serial dilution of plasmid, 2 ul of bacteria with the same plasmid, but without the tsDNA polymerase was added. A 10-fold dilution is not shown, but gives similar amplification to that seen with undiluted cells. One kb molecular weight markers are shown in the last lane.</p

ExCyto PCR of chromosomal integrants.

<p>Two µl of overnight cultures from chromosomal integrants were used for PCR with primers flanking the melAmelB bicistron integration site. Integrants show a band at 4.5 kb. Wild type (WT, last lane) has a band at 2 kb. One kb molecular weight markers are shown in the first lane.</p