20 research outputs found

    Effects of Protein Load Prior to the Main Meal of the Day: A Pilot Trial

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    Background: High protein diets increase satiety and may decrease energy intake. Many overweight people overeat in the evening. We hypothesized that ingesting protein prior to the evening meal may limit successive calorie intake and generate weight loss. Aims: To explore whether protein pre-load before the evening meal will lead to weight loss compared to eating as usual. Methods: 129 adults with a Body Mass Index (BMI) ≄25 reporting eating large evening meals were randomized to either consume a 20 g protein bar 30 minutes before their evening meal daily for two weeks (Protein pre-loading (PP) arm) or not (No protein pre-loading (NP) arm). Hunger ratings were recorded, immediately prior to each evening meal. Participants returned at the end of weeks one and two to provide their weight and rating of hunger and any changes in evening food consumption since baseline. Results: There was no significant difference in weight loss between the study arms (Week1 PP: -0.13 kg, [SD=0.74] vs. NP: -0.06 kg, [SD=0.75], not significant (NS); Week2 PP: +0.06 kg, [SD=0.82] vs. NP: -0.005 kg, [SD=0.82], NS). Participants in the PP arm reported less hunger before evening meals than those in the NP arm (Week1: 4.97 [SD=0.94] vs. 3.72[SD=0.65], p < .0011; Week2: 4.95 [SD=0.94] vs. 3.69[SD=0.71], p < .001). They also reported eating less at their evening meals (Week1: 2.59[SD=0.53] vs. 2.11[SD=0.54], p < .001; Week2: 2.63[SD=0.49] vs. 2.10[SD=0.50], p < .001). Conclusion: Consuming 20 g of protein before the evening meal reduced hunger and self-reported food intake in the evening, but had no effect on weight

    Harmonization and standardization of nucleus pulposus cell extraction and culture methods

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    Background: In vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or to aid in the development of new therapies. However, lab‐to‐lab variability jeopardizes the much‐needed progress in the field. Here, an international group of spine scientists collaborated to standardize extraction and expansion techniques for NP cells to reduce variability, improve comparability between labs and improve utilization of funding and resources. Methods: The most commonly applied methods for NP cell extraction, expansion, and re‐differentiation were identified using a questionnaire to research groups worldwide. NP cell extraction methods from rat, rabbit, pig, dog, cow, and human NP tissue were experimentally assessed. Expansion and re‐differentiation media and techniques were also investigated. Results: Recommended protocols are provided for extraction, expansion, and re‐differentiation of NP cells from common species utilized for NP cell culture. Conclusions: This international, multilab and multispecies study identified cell extraction methods for greater cell yield and fewer gene expression changes by applying species‐specific pronase usage, 60–100 U/ml collagenase for shorter durations. Recommendations for NP cell expansion, passage number, and many factors driving successful cell culture in different species are also addressed to support harmonization, rigor, and cross‐lab comparisons on NP cells worldwide

    Food & meal decision making in lockdown: how and who has Covid-19 affected?

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    The Covid-19 lockdown resulted in all but essential shops closing in many countries, with inevitable and immediate impact on food availability and choice. Reasons for specific food choices influence diet and mealtimes and can affect psychological and physical well-being. The current study aimed first to investigate whether individuals and families in the UK have changed their food choice motivations over lockdown and second to identify sub-groups in particular need of support in the event of future lockdowns. Two hundred and forty adult participants from the UK completed an online survey, consisting of a series of demographic questions, the Food Choice Questionnaire, the Family Mealtime Goals Questionnaire and some short open-ended questions. They were asked to consider their goals and motivations around food and mealtimes at two timepoints: before lockdown and Summer 2020. Results indicated that the sample placed more importance on health, weight control and mood when choosing their food after lockdown than they had before, and less importance on familiarity. A number of sub-groups were identified who may be particularly vulnerable to food-related challenges in future lockdowns including younger adults, parents and carers of children, those self-isolating and individuals who do not live within close proximity to food shops. These results are preliminary and larger sub-group sample sizes will be necessary to draw firm conclusions. Future research should consider the nature and impact of these challenges in more detail across a more varied population
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