Immunoblots of defatted, buffer D extracted peanut samples (numbers match Table 1) blotted with polyclonal galline anti-Ara antibodies.

<p>A) anti-Ara h 1, B) anti-Ara h 2, C) anti-Ara h 6 and D) anti-Ara h 3. Extracts were run on 14% reducing SDS-PAGE, 50μg per lane, and transferred to 0.22μm NC before blotting.</p

Inhibition immunoblots.

<p>A) Inhibition immunoblot of raw peanut (sample 38) with sera from peanut allergic individuals presorbed with boiled/fried peanut (sample 37). B) Inhibition immunoblot of boiled/fried peanut (sample 37) with sera from peanut allergic individuals presorbed with purified Ara h 2 protein (h2) or not presorbed (0). C) Inhibition immunoblot of raw peanut (sample 38) with sera 4 presorbed with purified Ara h 2 (5μg or 10μg) or Ara h 6 (5μg or 10μg). “P” indicates a sample was presorbed with pollen extract.</p

Immunoblots of defatted, buffer D extracted peanut samples (numbers match Table 1) blotted with serum from peanut allergic individuals.

<p>Serum was from individuals with a broad IgE repertoire (A, B), individuals with a more limited IgE repertoire (C, D), or an atopic (mold sensitive), non-food allergic patient with presumably no peanut-specific IgE (E). Extracts were run on 14% SDS-PAGE, 50μg per lane, and transferred to 0.22μm NC before blotting.</p

12% reducing SDS-PAGE of all 38 defatted, buffer D extracted peanut extracts.

<p>A raw peanut extract (Raw) from defatted peanut was included on each gel as an internal control. Lanes are labeled with extract number (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0157849#pone.0157849.t001" target="_blank">Table 1</a>). Each lane was loaded with 65μg of protein.</p

Subject Peanut Specific-IgE.

<p>Subject Peanut Specific-IgE.</p

Dot blot of all 38 defatted, buffer D extracted peanut samples (1μg/dot).

<p>(A) Dot blot layout. Samples were blotted with serum from an atopic (mold sensitive), non-food allergic patient with presumably no peanut-specific IgE (B) and a peanut allergic individual (C).</p

Comparison of chloroform/methanol defatted (df) and non-defatted (ndf), buffer D extracted raw and boiled/fried peanut samples.

<p>50μg protein (as determined using the Bradford assay on extracts prior to centrifugation) was loaded per lane on a 14% reducing SDS-PAGE. Lane labeled “spun” was loaded with non-defatted samples that had been centrifuged for one minute at 15,000 rpm in a benchtop eppendorf centrifuge. Arrows indicate differentially detected proteins.</p

Peanut Samples.

<p>Peanut Samples.</p

Association of serum adipokine tertiles with the presence of a hyperplastic polyp.

<p>Note: Model is adjusted for age and ever/never smoked.</p

Pearson correlation coefficients.

<p>Note: The top number is the Pearson correlation coefficient. The P values are shown under the correlation coefficient.</p